重组Anti-Cytokeratin 20抗体[SP33] (ab64090)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [SP33] to Cytokeratin 20
- Suitable for: IHC-P, IHC-Fr, ICC/IF, Flow Cyt
- Reacts with: Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-Cytokeratin 20抗体[SP33]
参阅全部 Cytokeratin 20 一抗 -
描述
兔单克隆抗体[SP33] to Cytokeratin 20 -
宿主
Rabbit -
经测试应用
适用于: IHC-P, IHC-Fr, ICC/IF, Flow Cytmore details -
种属反应性
与反应: Rat, Human
预测可用于: Mouse, Cow, Dog -
免疫原
Synthetic peptide within Human Cytokeratin 20 aa 400 to the C-terminus (C terminal). The exact sequence is proprietary.
Database link: P35900 -
阳性对照
- IHC-P: Human colon carcinoma tissue. IHC-Fr: Rat colon Tissue ICC/IF: HT-29 cells Flow Cyt: HT-29 cells
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading...
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纯度
Protein A/G purified -
纯化说明
Purified from TCS by protein A/G. -
克隆
单克隆 -
克隆编号
SP33 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab64090于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
IHC-P |
1/100.
Boil tissue section in citrate buffer pH 6.0 for 10 minutes followed by cooling at room temperature for 20 minutes. Incubate with primary antibody for 30 minutes at room temperature. |
|
IHC-Fr | (1) |
1/1000.
|
ICC/IF |
1/500.
|
|
Flow Cyt |
1/1700.
|
说明 |
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IHC-P
1/100. Boil tissue section in citrate buffer pH 6.0 for 10 minutes followed by cooling at room temperature for 20 minutes. Incubate with primary antibody for 30 minutes at room temperature. |
IHC-Fr
1/1000. |
ICC/IF
1/500. |
Flow Cyt
1/1700. |
靶标
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功能
Plays a significant role in maintaining keratin filament organization in intestinal epithelia. When phosphorylated, plays a role in the secretion of mucin in the small intestine. -
组织特异性
Expressed predominantly in the intestinal epithelium. Expressed in luminal cells of colonic mucosa. Also expressed in the Merkel cells of keratinized oral mucosa; specifically at the tips of some rete ridges of the gingival mucosa, in the basal layer of the palatal mucosa and in the taste buds of lingual mucosa. -
序列相似性
Belongs to the intermediate filament family. -
发展阶段
First detected at embryonic week 8 in individual 'converted' simple epithelial cells of the developing intestinal mucosa. In later fetal stages, synthesis extends over most goblet cells and a variable number of villus enterocytes. In the developing gastric and intestinal mucosa, expressed in all enterocytes and goblet cells as well as certain 'low-differentiated' columnar cells, whereas the neuroendocrine and Paneth cells are negative. -
翻译后修饰
Hyperphosphorylation at Ser-13 occurs during the early stages of apoptosis but becomes less prominent during the later stages. Phosphorylation at Ser-13 also increases in response to stress brought on by cell injury.
Proteolytically cleaved by caspases during apoptosis. Cleavage occurs at Asp-228. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 54474 Human
- Entrez Gene: 66809 Mouse
- Entrez Gene: 286912 Rat
- Omim: 608218 Human
- SwissProt: P35900 Human
- SwissProt: Q9D312 Mouse
- SwissProt: P25030 Rat
- Unigene: 84905 Human
see all -
别名
- CD20 antibody
- CK 20 antibody
- CK-20 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat colon tissue sections labeling Cytokeratin 20 with ab64090 at 1/100 dilution (17.9 µg/ml). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on rat colon, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab64090 for 30 mins at room temperature. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human colon carcinoma tissue sections labeling Cytokeratin 20 with ab64090 at 1/100 dilution (17.9 µg/ml). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on human colon carcinoma, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab64090 for 30 mins at room temperature. -
Formalin-fixed, paraffin-embedded human colon carcinoma tissue stained for Cytokeratin 20 with ab64090 at a 1/100 dilution in immunohistochemical analysis.
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Immunohistochemistry (Frozen) analysis of rat colon tissue section labeling Cytokeratin 20 with purified ab64090 at 1/1000 (1.8 µg/ml). Sections were fixed in 4% paraformaldehyde and permeabilized with 0.2% Triton X-100. Antigen retrieval was heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
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Immunocytochemistry/ Immunofluorescence analysis of HT-29 (human colorectal adenocarcinoma epithelial cell) cells labeling Cytokeratin 20 with purified ab64090 at 1/500 (3.58 µg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
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Flow cytometry analysis of HT-29 (human colorectal adenocarcinoma) labeling Cytokeratin 20 with purified ab64090 at 1/1700 dilution (1.05 µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as a secondary antibody. Isotype control -Rabbit monoclonal IgG (ab172730) (Black). Unlableled control -Unlabelled cells (blue).
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab64090 被引用在 2 文献中.
- Engel RM et al. Modeling colorectal cancer: A bio-resource of 50 patient-derived organoid lines. J Gastroenterol Hepatol 37:898-907 (2022). PubMed: 35244298
- Abdel-Hamid AAM & Firgany AEL Dysplastic urothelial changes accompany empagliflozin administration in urinary bladder of experimental diabetes. Int J Exp Pathol 100:369-377 (2019). PubMed: 32040227