Anti-Cytokeratin 20抗体[Ks20.8] (ab854)

概述

  • 产品名称Anti-Cytokeratin 20抗体[Ks20.8]
    参阅全部 Cytokeratin 20 一抗
  • 描述
    小鼠单克隆抗体[Ks20.8] to Cytokeratin 20
  • 特异性This antibody is highly specific to cytokeratin 20 and shows no cross-reaction with other intermediate filament proteins. It is essentially non-reactive in squamous cell carcinomas and adenocarcinomas of the breast, lung, and endometrium, non-mucinous tumors of the ovary and small cell carcinomas.
  • 经测试应用适用于: Flow Cyt, WB, IHC-Fr, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Cytoskeletal preparation from micro-dissected villi of human duodenal mucosa.

  • 阳性对照
    • Colon carcinoma. IF/ICC: Jeg3 cell line.

性能

应用

Our Abpromise guarantee covers the use of ab854 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt 1/50. (Also see PMID: 20332776)

WB Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. This antibody may be diluted to a titer of 1:50-1:100 in an ABC method. We suggest an incubation period of 30-60 minutes at room temperature.
ICC/IF 1/200.

靶标

  • 功能Plays a significant role in maintaining keratin filament organization in intestinal epithelia. When phosphorylated, plays a role in the secretion of mucin in the small intestine.
  • 组织特异性Expressed predominantly in the intestinal epithelium. Expressed in luminal cells of colonic mucosa. Also expressed in the Merkel cells of keratinized oral mucosa; specifically at the tips of some rete ridges of the gingival mucosa, in the basal layer of the palatal mucosa and in the taste buds of lingual mucosa.
  • 序列相似性Belongs to the intermediate filament family.
  • 发展阶段First detected at embryonic week 8 in individual 'converted' simple epithelial cells of the developing intestinal mucosa. In later fetal stages, synthesis extends over most goblet cells and a variable number of villus enterocytes. In the developing gastric and intestinal mucosa, expressed in all enterocytes and goblet cells as well as certain 'low-differentiated' columnar cells, whereas the neuroendocrine and Paneth cells are negative.
  • 翻译后修饰Hyperphosphorylation at Ser-13 occurs during the early stages of apoptosis but becomes less prominent during the later stages. Phosphorylation at Ser-13 also increases in response to stress brought on by cell injury.
    Proteolytically cleaved by caspases during apoptosis. Cleavage occurs at Asp-228.
  • 细胞定位Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CD20 antibody
    • CK 20 antibody
    • CK-20 antibody
    • CK20 antibody
    • Cytokeratin-20 antibody
    • Cytokeratin20 antibody
    • K1C20_HUMAN antibody
    • K20 antibody
    • KA20 antibody
    • Keratin 20 antibody
    • keratin 20, type I antibody
    • keratin 21, rat, homolog of antibody
    • Keratin antibody
    • Keratin type I cytoskeletal 20 antibody
    • Keratin-20 antibody
    • Keratin20 antibody
    • KRT 20 antibody
    • KRT 21 antibody
    • KRT20 antibody
    • KRT21 antibody
    • MGC35423 antibody
    • OTTHUMP00000164518 antibody
    • Protein IT antibody
    • type I cytoskeletal 20 antibody
    see all

Anti-Cytokeratin 20 antibody [Ks20.8] 图像

  • ICC/IF image of ab854 stained Jeg3 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab854, 1/200 dilution) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM

  • ab854 staining cytokeratin in human colon cancer tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin embedded tissue sections).

  • Immunohistochemical analysis of human colon epithelium, staining Cytokeratin 20 with ab854.

  • Overlay histogram showing LoVo cells stained with ab854 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab854, 1/50 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

Anti-Cytokeratin 20 antibody [Ks20.8] (ab854)参考文献

This product has been referenced in:
  • Fang DD  et al. Expansion of CD133(+) colon cancer cultures retaining stem cell properties to enable cancer stem cell target discovery. Br J Cancer : (2010). Flow Cyt ; Human . Read more (PubMed: 20332776) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Tissue lysate - whole (Colon Carcinoma)
Gel Running Conditions Reduced Denaturing (12)
Loading amount 30 µg
Specification Colon Carcinoma
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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提交于 Aug 22 2016

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Organoid)
Permeabilization Yes - 0.3% Triton
Specification Organoid
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative Paraformaldehyde
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提交于 Mar 10 2016

Thank you for your enquiry.

I can confirm that ab854 Anti-Cytokeratin 20 antibody [Ks20.8]is provided as tissue culture supernatant, which we would expect to have a pink-red coloration. Therefore, I would not expect this to affect the antibo...

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This antibody has been tested for use in IHC, but to our knowledge has not yet been used in ELISA.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"