Cytokeratin 19 overexpression 293T裂解液(全细胞) (ab94061)

概述

  • 产品名称Cytokeratin 19 overexpression 293T裂解液(全细胞)
    参阅全部 Cytokeratin 19 细胞裂解液
  • 描述
    Cytokeratin 19 overexpression裂解液
  • 常规说明ab94061 is a 293T cell transfected lysate in which Human Cytokeratin 19 has been transiently over-expressed using a pCMV-Cytokeratin 19 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more detailed how the transfected lysate was prepared view preparation notes
  • 经测试应用适用于: WBmore details

性能

  • 背景Developmental stage: Present in hair follicles at all stages of development. Domain: This keratin differs from all other IF proteins in lacking the C-terminal tail domain. Function: Involved in the organization of myofibers. Together with KRT8, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle. Similarity: Belongs to the intermediate filament family. Tissue specificity: Expressed in a defined zone of basal keratinocytes in the deep outer root sheath of hair follicles. Also observed in sweat gland and mammary gland ductal and secretory cells, bile ducts, gastrointestinal tract, bladder urothelium, oral epithelia, esophagus, ectocervical epithelium (at protein level). Expressed in epidermal basal cells, in nipple epidermis and a defined region of the hair follicle. Also seen in a subset of vascular wall cells in both the veins and artery of human umbilical cord, and in umbilical cord vascular smooth muscle. Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma in structures that contain dystrophin and spectrin.

应用

Our Abpromise guarantee covers the use of ab94061 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent dilution.

Cytokeratin 19 overexpression 293T lysate (whole cell) 图像

  • ab94061 at 15µg/lane on an SDS-PAGE gel.
  • All lanes : Anti-Cytokeratin 19 antibody (ab90440) at 1/500 dilution

    Lane 1 : Cytokeratin 19 overexpression 293T lysate (whole cell) (ab94061)
    Lane 2 : 293T non-transfected lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

Cytokeratin 19 overexpression 293T lysate (whole cell) (ab94061)参考文献

ab94061 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Questions for ab94061.
Please use the link above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"