Cytokeratin 19 overexpression 293T裂解物(whole cell) (ab94061)
概述
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产品名称
Cytokeratin 19 overexpression 293T裂解物(whole cell)
参阅全部 Cytokeratin 19 细胞裂解液 -
常规说明
ab94061 is a 293T cell transfected lysate in which Human Cytokeratin 19 has been transiently over-expressed using a pCMV-Cytokeratin 19 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more detailed how the transfected lysate was prepared view preparation notes
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经测试应用
适用于: WBmore details
性能
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Mycoplasma free
Yes -
形式
Liquid -
存放说明
Shipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles. -
存储溶液
Constituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.788% Tris HCl, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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研究领域
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背景
Developmental stage: Present in hair follicles at all stages of development. Domain: This keratin differs from all other IF proteins in lacking the C-terminal tail domain. Function: Involved in the organization of myofibers. Together with KRT8, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle. Similarity: Belongs to the intermediate filament family. Tissue specificity: Expressed in a defined zone of basal keratinocytes in the deep outer root sheath of hair follicles. Also observed in sweat gland and mammary gland ductal and secretory cells, bile ducts, gastrointestinal tract, bladder urothelium, oral epithelia, esophagus, ectocervical epithelium (at protein level). Expressed in epidermal basal cells, in nipple epidermis and a defined region of the hair follicle. Also seen in a subset of vascular wall cells in both the veins and artery of human umbilical cord, and in umbilical cord vascular smooth muscle. Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma in structures that contain dystrophin and spectrin.
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab94061于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use at an assay dependent dilution.
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说明 |
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WB
Use at an assay dependent dilution. |
图片
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ab94061 at 15µg/lane on an SDS-PAGE gel.
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All lanes : Anti-Cytokeratin 19 antibody (ab90440) at 1/500 dilution
Lane 1 :Cytokeratin 19 overexpression 293T lysate (whole cell) (ab94061)
Lane 2 : 293T non-transfected lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab94061 尚未被引用在任何文献中。