FITC荧光Anti-Cytokeratin 19抗体[SB39g] (ab87014)
Key features and details
- FITC Mouse monoclonal [SB39g] to Cytokeratin 19
- Suitable for: ICC, Flow Cyt (Intra)
- Reacts with: Human
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG2a
概述
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产品名称
FITC荧光Anti-Cytokeratin 19抗体[SB39g]
参阅全部 Cytokeratin 19 一抗 -
描述
FITC荧光小鼠单克隆抗体[SB39g] to Cytokeratin 19 -
宿主
Mouse -
偶联物
FITC. Ex: 493nm, Em: 528nm -
经测试应用
适用于: ICC, Flow Cyt (Intra)more details -
种属反应性
与反应: Human -
免疫原
Recombinant fragment (Human)
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阳性对照
- Flow Cyt (Intra): HT29 cells ELISA and IHC: HT29 and HCC38 cells WB: HT29 and HCC38 cell lysates
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常规说明
Monoclonal antibodies to cytokeratin proteins can be useful markers for tumor identification and classification.
This product was changed from ascites to tissue culture supernatant on 17th September 2019. Lot numbers higher than GR3224453 are from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. Store In the Dark. -
存储溶液
pH: 7.4
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading...
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纯度
Tissue culture supernatant -
Primary antibody说明
Monoclonal antibodies to cytokeratin proteins can be useful markers for tumor identification and classification. -
克隆
单克隆 -
克隆编号
SB39g -
同种型
IgG2a -
研究领域
相关产品
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab87014于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ICC |
Use at an assay dependent concentration.
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Flow Cyt (Intra) |
Use at an assay dependent concentration.
ab91362 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
说明 |
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ICC
Use at an assay dependent concentration. |
Flow Cyt (Intra)
Use at an assay dependent concentration. ab91362 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
靶标
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功能
Involved in the organization of myofibers. Together with KRT8, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle. -
组织特异性
Expressed in a defined zone of basal keratinocytes in the deep outer root sheath of hair follicles. Also observed in sweat gland and mammary gland ductal and secretory cells, bile ducts, gastrointestinal tract, bladder urothelium, oral epithelia, esophagus, ectocervical epithelium (at protein level). Expressed in epidermal basal cells, in nipple epidermis and a defined region of the hair follicle. Also seen in a subset of vascular wall cells in both the veins and artery of human umbilical cord, and in umbilical cord vascular smooth muscle. Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma in structures that contain dystrophin and spectrin. -
序列相似性
Belongs to the intermediate filament family. -
发展阶段
Present in hair follicles at all stages of development. -
结构域
This keratin differs from all other IF proteins in lacking the C-terminal tail domain. - Information by UniProt
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数据库链接
- Entrez Gene: 3880 Human
- Omim: 148020 Human
- SwissProt: P08727 Human
- Unigene: 654568 Human
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别名
- 40 kDa keratin intermediate filament antibody
- CK 19 antibody
- CK-19 antibody
see all
图片
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Overlay histogram showing MCF-7 cells stained with ab87014 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab87014, 0.5µg/1x106 cells) for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a (FITC) (1µg/1x106 cells) for 30 min at 22°C. Acquisition of >5,000 events was performed. This antibody gave a positive signal in MCF-7 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
This image was generated using the ascites version of the product.
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Ab87014 staining Cytokeratin 19 in MIA PaCa-2 cells ( Human pancreatic carcinoma cell line) .
This image was generated using the ascites version of the product.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (6)
ab87014 被引用在 6 文献中.
- Liu J et al. Human Amniotic Epithelial Cells Promote the Proliferation of Human Corneal Endothelial Cells by Regulating Telomerase Activity via the Wnt/ß-catenin Pathway. Curr Eye Res 46:159-167 (2021). PubMed: 32631162
- Peraldo-Neia C et al. A Novel Multidrug-Resistant Cell Line from an Italian Intrahepatic Cholangiocarcinoma Patient. Cancers (Basel) 13:N/A (2021). PubMed: 33922695
- Yue C et al. Dynamic change of PD-L1 expression on circulating tumor cells in advanced solid tumor patients undergoing PD-1 blockade therapy. Oncoimmunology 7:e1438111 (2018). PubMed: 29900038
- Zhou M et al. Precisely Enumerating Circulating Tumor Cells Utilizing a Multi-Functional Microfluidic Chip and Unique Image Interpretation Algorithm. Theranostics 7:4710-4721 (2017). PubMed: 29187898
- Santoro A et al. Relationship between CK19 expression, deregulation of normal keratinocyte differentiation pattern and high risk-human papilloma virus infection in oral and oropharyngeal squamous cell carcinoma. Infect Agent Cancer 10:46 (2015). Flow Cyt . PubMed: 26672675
- Cardinale V et al. Profiles of cancer stem cell subpopulations in cholangiocarcinomas. Am J Pathol 185:1724-39 (2015). PubMed: 25892683