重组
RabMAb

Anti-Cytokeratin 19抗体[EP1580Y] (ab52625)

概述

  • 产品名称
    Anti-Cytokeratin 19抗体[EP1580Y]
    参阅全部 Cytokeratin 19 一抗
  • 描述
    兔单克隆抗体[EP1580Y] to Cytokeratin 19
  • 经测试应用
    适用于: ICC/IF, IHC-Fr, WB, Flow Cyt, IHC-Pmore details
    不适用于: IP
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide within Human Cytokeratin 19 (C terminal). The exact sequence is proprietary.
    Database link: P08727

  • 阳性对照
    • WB: HepG2 and NIH/3T3 cell lysates. IHC-P: Human skin, breast carcinoma, kidney carcinoma, endometrial carcinoma and gastric adenocarcinoma tissues. ICC/IF: HepG2 and MCF-7 cells. Flow Cyt: MCF-7 and HeLa cells.
  • 常规说明

    A trial size is available to purchase for this antibody.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

性能

应用

Our Abpromise guarantee covers the use of ab52625 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/200 - 1/500.

For unpurified, use 1/50.

IHC-Fr Use at an assay dependent concentration.
WB 1/50000 - 1/200000. Detects a band of approximately 44 kDa (predicted molecular weight: 44 kDa).

For unpurified, use 1/10000 - 1/50000.

Flow Cyt 1/30 - 1/80.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IHC-P 1/400 - 1/800. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See protocols (link: http://www.abcam.com/protocols/ihc-antigen-retrieval-protocol).

For unpurified, use at 1/100.

  • 应用说明
    Is unsuitable for IP.
  • 靶标

    • 功能
      Involved in the organization of myofibers. Together with KRT8, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.
    • 组织特异性
      Expressed in a defined zone of basal keratinocytes in the deep outer root sheath of hair follicles. Also observed in sweat gland and mammary gland ductal and secretory cells, bile ducts, gastrointestinal tract, bladder urothelium, oral epithelia, esophagus, ectocervical epithelium (at protein level). Expressed in epidermal basal cells, in nipple epidermis and a defined region of the hair follicle. Also seen in a subset of vascular wall cells in both the veins and artery of human umbilical cord, and in umbilical cord vascular smooth muscle. Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma in structures that contain dystrophin and spectrin.
    • 序列相似性
      Belongs to the intermediate filament family.
    • 发展阶段
      Present in hair follicles at all stages of development.
    • 结构域
      This keratin differs from all other IF proteins in lacking the C-terminal tail domain.
    • Information by UniProt
    • 数据库链接
    • 别名
      • 40 kDa keratin intermediate filament antibody
      • CK 19 antibody
      • CK-19 antibody
      • CK19 antibody
      • Cytokeratin 19 antibody
      • Cytokeratin-19 antibody
      • K19 antibody
      • K1C19_HUMAN antibody
      • K1CS antibody
      • Keratin 19 antibody
      • Keratin type I 40 kD antibody
      • Keratin type I 40kD antibody
      • Keratin type I cytoskeletal 19 antibody
      • Keratin, type I cytoskeletal 19 antibody
      • Keratin, type I, 40 kd antibody
      • Keratin-19 antibody
      • KRT19 antibody
      • MGC15366 antibody
      see all

    图片

    • Immunocytochemistry/Immunofluorescence analysis of HepG2 (human hepatocellular carcinoma) cells labelling Cytokeratin 19 (green) with purified ab52625 at 1/500. Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. Cells were counter-stained with ab7291, anti-Tubulin (mouse mAb) at 1/1000 followed by ab150120 AlexaFluor®594 goat anti-mouse secondary (1/1000). Nuclei were counterstained with DAPI (blue).

      For negative control 1, rabbit primary antibody and anti-mouse secondary antibody (ab150120) were used. For negative control 2, ab7291 (mouse primary antibody) was used followed by anti-rabbit secondary antibody (ab150077).  

    • All lanes : Anti-Cytokeratin 19 antibody [EP1580Y] (ab52625) at 1/45000 dilution (purified)

      Lane 1 : HepG2 cell lysate
      Lane 2 : NIH/3T3 cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      HRP goat anti-rabbit (H+L) at 1/1000 dilution

      Predicted band size : 44 kDa
      Observed band size : 40 kDa (why is the actual band size different from the predicted?)

      Blocking buffer: 5% NFDM/TBST

      Dilution buffer: 5% NFDM/TBST

    • Immunohistochemical staining of paraffin-embedded human skin with purified ab52625 at a dilution of 1/400. A pre-diluted HRP polymer for rabbit/mouse IgG was used as the secondary antibody and the sample was counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

    • ab52625 staining Cytokeratin 19 in the human cell line MCF-7 (human breast carcinoma) by flow cytometry. Cells were fixed with 4% paraformaldehyde, permeabilized with 90% methanol and the sample was incubated with the primary antibody at a dilution of 1/80. A goat anti rabbit IgG (Alexa Fluor® 488) at a dilution of 1/2000 was used as the secondary antibody.

      Isoytype control: Rabbit monoclonal IgG (Black)

      Unlabelled control: Cell without incubation with primary antibody and secondary antibody (Blue)

    • Immunofluorescent staining of MCF-7 cells (fixed in 4% PFA, permeabilized with 0.1% Triton X 100) using purified ab52625 at a dilution of 1/200. An Alexa Fluor® 488 goat anti-rabbit antibody was used as the secondary at a dilution of 1/200 and the cells were counter stained with DAPI.

    • Unpurified ab52625 showing positive staining in Breast carcinoma tissue.

    • Overlay histogram showing HeLa cells stained with unpurified ab52625 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab52625, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

    • Unpurified ab52625 showing positive staining in Endometrial carcinoma tissue.

    • Unpurified ab52625 showing negative staining in Glioma tissue.

    • Unpurified ab52625 showing positive staining in Gastric adenocarcinoma tissue.

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    文献

    This product has been referenced in:
    • Kim JY  et al. Apamin suppresses biliary fibrosis and activation of hepatic stellate cells. Int J Mol Med 39:1188-1194 (2017). IHC-P ; Mouse . Read more (PubMed: 28405682) »
    • Feng F  et al. Guidance to rational use of pharmaceuticals in gallbladder sarcomatoid carcinoma using patient-derived cancer cells and whole exome sequencing. Oncotarget 8:5349-5360 (2017). IHC ; Human . Read more (PubMed: 28029662) »

    See all 31 Publications for this product

    客户评价及客户问答

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (Pancreas Tissue)
    Permeabilization
    Yes - Tween 20
    Specification
    Pancreas Tissue
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
    Fixative
    Formaldehyde
    Username

    Dhruvit Sutaria

    Verified customer

    提交于 Jun 28 2017

    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Mouse salivary gland)
    Permeabilization
    Yes - 0.1% Triton
    Specification
    Mouse salivary gland
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2000µg/mL · Temperature: 21°C
    Fixative
    Formaldehyde
    Username

    Dr. Isabelle Miletich

    Verified customer

    提交于 Mar 07 2017

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (tongue)
    Antigen retrieval step
    Heat mediated
    Permeabilization
    No
    Specification
    tongue
    Fixative
    Formaldehyde
    Username

    Abcam user community

    Verified customer

    提交于 Aug 26 2016

    Application
    Immunocytochemistry/ Immunofluorescence
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Sample
    Human Cell (HCC1143 cell lines)
    Specification
    HCC1143 cell lines
    Permeabilization
    Yes - 0.3% Triton X-100 in TBS
    Fixative
    Formaldehyde
    Username

    Abcam user community

    Verified customer

    提交于 Feb 25 2015

    Application
    Flow Cytometry
    Fixation
    Paraformaldehyde
    Permeabilization
    Yes - 0.2% Triton X-100 in PBS
    Sample
    Human Cell (CK19 positive and negative cell lines)
    Specification
    CK19 positive and negative cell lines
    Gating Strategy
    live cells, doubled were excluded based on FSC - SSC profile
    Preparation
    Cell harvesting/tissue preparation method: Cells were harvested with trypsin and fixed with 2% PFA / PBS for 15 min on ice. Following cell fixation, PFA was removed, cells were washed with ice-cold PBS, permeabilised and blocked with 0.2% Triton X-100 / 5% FBS / PBS for 30 min on ice prior to staining
    Sample buffer: PBS
    Username

    Abcam user community

    Verified customer

    提交于 Feb 05 2015

    Application
    Western blot
    Loading amount
    30 µg
    Gel Running Conditions
    Reduced Denaturing (4-12)
    Sample
    Human Cell lysate - whole cell (panel of breast cancer cell lines)
    Specification
    panel of breast cancer cell lines
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
    Username

    Abcam user community

    Verified customer

    提交于 Dec 18 2014

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Liver)
    Specification
    Liver
    Fixative
    Formaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrat pH 6,0
    Permeabilization
    Yes - 0,5% Tween 20
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Mrs. Sabine Pohl

    Verified customer

    提交于 Jul 14 2008

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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