Anti-Cytokeratin 19抗体(ab84632)

概述

  • 产品名称Anti-Cytokeratin 19抗体
    参阅全部 Cytokeratin 19 一抗
  • 描述
    兔多克隆抗体to Cytokeratin 19
  • 经测试应用适用于: WB, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig
  • 免疫原

    Synthetic peptide corresponding to a region within N terminal amino acids 2-51 (TSYSYRQSSA TSSFGGLGGG SVRFGPGVAF RAPSIHGGSG GRGVSVSSAR) of Human Cytokeratin 19 (NP_002267).

  • 阳性对照
    • Human fetal lung lysate. This antibody gave a positive result in IHC in the following FFPE tissue: Human normal colon. IF/ICC: HCT116 cell line.

性能

应用

Our Abpromise guarantee covers the use of ab84632 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 44 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 5 µg/ml.
ICC/IF Use a concentration of 1 µg/ml.

靶标

  • 功能Involved in the organization of myofibers. Together with KRT8, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.
  • 组织特异性Expressed in a defined zone of basal keratinocytes in the deep outer root sheath of hair follicles. Also observed in sweat gland and mammary gland ductal and secretory cells, bile ducts, gastrointestinal tract, bladder urothelium, oral epithelia, esophagus, ectocervical epithelium (at protein level). Expressed in epidermal basal cells, in nipple epidermis and a defined region of the hair follicle. Also seen in a subset of vascular wall cells in both the veins and artery of human umbilical cord, and in umbilical cord vascular smooth muscle. Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma in structures that contain dystrophin and spectrin.
  • 序列相似性Belongs to the intermediate filament family.
  • 发展阶段Present in hair follicles at all stages of development.
  • 结构域This keratin differs from all other IF proteins in lacking the C-terminal tail domain.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 40 kDa keratin intermediate filament antibody
    • CK 19 antibody
    • CK-19 antibody
    • CK19 antibody
    • Cytokeratin 19 antibody
    • Cytokeratin-19 antibody
    • K19 antibody
    • K1C19_HUMAN antibody
    • K1CS antibody
    • Keratin 19 antibody
    • Keratin type I 40 kD antibody
    • Keratin type I 40kD antibody
    • Keratin type I cytoskeletal 19 antibody
    • Keratin, type I cytoskeletal 19 antibody
    • Keratin, type I, 40 kd antibody
    • Keratin-19 antibody
    • KRT19 antibody
    • MGC15366 antibody
    see all

Anti-Cytokeratin 19 antibody 图像

  • ICC/IF image of ab84632 stained HCT116 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab84632, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM

  • Anti-Cytokeratin 19 antibody (ab84632) at 1 µg/ml (in 5% skim milk / PBS buffer) + human fetal lung lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 44 kDa
    Observed band size : 44 kDa
    Gel concentration: 12%
  • Immunohistochemistry of paraffin embedded bile duct tissue, tested with an antibody dilution of 5 µg/ml (ab84632)

  • Immunohistochemistry of paraffin embedded placenta tissue, tested with an antibody dilution of 5 µg/ml (ab84632)

  • Immunohistochemistry of paraffin embedded skin tissue, tested with an antibody dilution of 5 µg/ml (ab84632)

  • IHC image of Cytokeratin 19 staining in Human normal colon formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab84632, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-Cytokeratin 19 antibody (ab84632)参考文献

ab84632 has not yet been referenced specifically in any publications.

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