Anti-Cytokeratin 19抗体(ab15463)

概述

  • 产品名称
    Anti-Cytokeratin 19抗体
    参阅全部 Cytokeratin 19 一抗
  • 描述
    兔多克隆抗体to Cytokeratin 19
  • 经测试应用
    适用于: IHC - Wholemount, WB, IHC-P, IHC-Frmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) C terminal (Human).

  • 阳性对照
    • Skin.

性能

应用

Our Abpromise guarantee covers the use of ab15463 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC - Wholemount Use at an assay dependent concentration. Used at a dilution of 1/200 for 24 hr on mouse whole mounts of tail skin (see Abreview submitted by Jesus Espada).
WB Use a concentration of 1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 40 kDa).
IHC-P 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Boil in citrate buffer for 10 minutes, cool at room temperature for 20 minutes. After blocking, incubate primary antibody 1:100 for 10 minutes at room temperature.

IHC-Fr 1/50.

靶标

  • 功能
    Involved in the organization of myofibers. Together with KRT8, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.
  • 组织特异性
    Expressed in a defined zone of basal keratinocytes in the deep outer root sheath of hair follicles. Also observed in sweat gland and mammary gland ductal and secretory cells, bile ducts, gastrointestinal tract, bladder urothelium, oral epithelia, esophagus, ectocervical epithelium (at protein level). Expressed in epidermal basal cells, in nipple epidermis and a defined region of the hair follicle. Also seen in a subset of vascular wall cells in both the veins and artery of human umbilical cord, and in umbilical cord vascular smooth muscle. Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma in structures that contain dystrophin and spectrin.
  • 序列相似性
    Belongs to the intermediate filament family.
  • 发展阶段
    Present in hair follicles at all stages of development.
  • 结构域
    This keratin differs from all other IF proteins in lacking the C-terminal tail domain.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 40 kDa keratin intermediate filament antibody
    • CK 19 antibody
    • CK-19 antibody
    • CK19 antibody
    • Cytokeratin 19 antibody
    • Cytokeratin-19 antibody
    • K19 antibody
    • K1C19_HUMAN antibody
    • K1CS antibody
    • Keratin 19 antibody
    • Keratin type I 40 kD antibody
    • Keratin type I 40kD antibody
    • Keratin type I cytoskeletal 19 antibody
    • Keratin, type I cytoskeletal 19 antibody
    • Keratin, type I, 40 kd antibody
    • Keratin-19 antibody
    • KRT19 antibody
    • MGC15366 antibody
    see all

图片

  • Human skin stained with anti-Cytokeratin 19 antibody (ab15463).
  • All lanes : Anti-Cytokeratin 19 antibody (ab15463) at 1 µg/ml

    Lane 1 : Human placenta tissue lysate - total protein (ab29745)
    Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 3 : MDA-MB-361 (Human breast adenocarcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Predicted band size : 40 kDa
    Observed band size : 45 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 75 kDa. We are unsure as to the identity of these extra bands.
  • ab15463 staining Cytokeratin 19 in human colon tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 2% BSA for 10 minutes at 21°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/500 in blocking buffer) for 16 hours at 22°C. A Biotin-conjugated Goat anti-rabbit IgG polyclonal (1/300) was used as the secondary antibody.

    See Abreview

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse developing skin tissue labeling Cytokeratin 19 with ab15463 at 1/500 dilution. The tissue was fixed with formaldehyde; heat-mediated antigen retrieval was performed using a citric acid. Tissue was blocked with 2% BSA for 10 minutes at 21°C. A polyclonal goat anti-rabbit IgG biotin conjugated antibody was used ar 1/300 dilution.  Image shows strong immunopositivity in the cytoplasm of basal cells of the epidermis and a moderate hair follicle cell positivity.

    See Abreview

  • This image shows formalin-fixed mouse skin stained with Cytokeratin 19 antibody (1/50 dilution) after pepsin digestion.
  • ab15463 staining Cytokeratin 19 in the Mouse skin tissue sections by IHC-Fr (Frozen Sections). Tissue samples were fixed with methanol and blocked with 3% Goat serum for 30 minutes at 20°C. The sample was incubated with primary antibody (1/50 in PBS) at 4°C for 14 hours. An HRP-conjugated goat polyclonal to rabbit IgG (1/100) was used as secondary antibody.

    See Abreview

文献

This product has been referenced in:
  • Furukawa S  et al. Databases for technical aspects of immunohistochemistry. J Toxicol Pathol 30:79-107 (2017). IHC ; Rat . Read more (PubMed: 28190929) »
  • Shivakumar P  et al. Preferential TNFa signaling via TNFR2 regulates epithelial injury and duct obstruction in experimental biliary atresia. JCI Insight 2:e88747 (2017). IF ; Mouse . Read more (PubMed: 28289704) »

See all 27 Publications for this product

客户评价及客户问答

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (pancreas)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mm sodium citrate PH 6.0
Permeabilization
No
Specification
pancreas
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

提交于 Apr 04 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Colon)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Specification
Colon
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
Fixative
Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

提交于 Feb 13 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Developing skin)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Specification
Developing skin
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
Fixative
Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

提交于 Dec 02 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Liver)
Specification
Liver
Fixative
Formalin
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer
Permeabilization
No
Blocking step
BiocareMedical's Rodent Block M as blocking agent for 20 minute(s) · Concentration: 100% · Temperature: 23°C
Username

Abcam user community

Verified customer

提交于 Mar 05 2013

Thank you for taking time to contact us. I am sorry to hear that this antibody is not providing satisfactory results.

The details provided will enable us to investigate this case and will provide us with vital information for monitoring prod...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (Liver)
Specification
Liver
Fixative
Formaldehyde
Blocking step
PBS + 0.1% Triton X-100, 1% BSA, 10% goat serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Mr. justin kenkel

Verified customer

提交于 Jul 27 2012

Thank you for taking the time to complete our questionnaire and sending me the publication. I am sorry to hear you have had difficulty obtaining satisfactory results from these antibodies.

The details you have kindly provided will enable us ...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Mammary Gland duct)
Specification
Mammary Gland duct
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% Triton X-100
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 23°C
Username

Abcam user community

Verified customer

提交于 Mar 20 2012

Thank you for contacting us.

I am sorry to hear that the antibody is giving you problems.

As we discussed over the phone, please let me know what staining patternthe MCF7 cells give and we can discuss further how to proceed.
...

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Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this...

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1-10 of 32 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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