Anti-Cytokeratin 18 (phospho S33)抗体(ab75747)

概述

  • 产品名称
    Anti-Cytokeratin 18 (phospho S33)抗体
    参阅全部 Cytokeratin 18 一抗
  • 描述
    兔多克隆抗体to Cytokeratin 18 (phospho S33)
  • 特异性
    ab75747 detects endogenous levels of Cytokeratin 18 only when phosphorylated at serine 33.
  • 经测试应用
    适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic phosphopeptide derived from human Cytokeratin 18 around the phosphorylation site of serine 33 (A-A-SP-V-Y).

  • 阳性对照
    • Human breast carcinoma tissue; extracts from A431 cells and EC-304 cells.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 纯化说明
    ab75747 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab75747 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Predicted molecular weight: 48 kDa.
IHC-P 1/50 - 1/100.

靶标

  • 功能
    Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.
  • 组织特异性
    Expressed in colon, placenta, liver and very weakly in exocervix. Increased expression observed in lymph nodes of breast carcinoma.
  • 疾病相关
    Defects in KRT18 are a cause of cirrhosis (CIRRH) [MIM:215600].
  • 序列相似性
    Belongs to the intermediate filament family.
  • 翻译后修饰
    Phosphorylation at Ser-34 increases during mitosis. Hyperphosphorylated at Ser-53 in diseased cirrhosis liver. Phosphorylation increases by IL-6.
    Proteolytically cleaved by caspases during epithelial cell apoptosis. Cleavage occurs at Asp-238 by either caspase-3, caspase-6 or caspase-7.
    O-glycosylated at multiple sites; glycans consist of single N-acetylglucosamine residues.
  • 细胞定位
    Cytoplasm > perinuclear region.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Cell proliferation inducing gene 46 protein antibody
    • Cell proliferation inducing protein 46 antibody
    • Cell proliferation-inducing gene 46 protein antibody
    • CK 18 antibody
    • CK-18 antibody
    • CK18 antibody
    • CYK 18 antibody
    • CYK18 antibody
    • Cytokeratin 18 antibody
    • Cytokeratin endo B antibody
    • Cytokeratin-18 antibody
    • K 18 antibody
    • K18 antibody
    • K1C18_HUMAN antibody
    • KA18 antibody
    • Keratin 18 antibody
    • Keratin 18, type I antibody
    • Keratin D antibody
    • keratin, type I cytoskeletal 18 antibody
    • Keratin-18 antibody
    • Krt18 antibody
    see all

图片

  • ab75747, at a 1/50 dilution, staining Cytokeratin 18 in paraffin embedded human breast carcinoma tissue by Immunohistochemistrytissue by Immunohistochemsitry in the absence (left image) or presence (right image) of the immunizing peptide.
  • All lanes : Anti-Cytokeratin 18 (phospho S33) antibody (ab75747) at 1/500 dilution

    Lane 1 : A431 cell extracts with immunising peptide
    Lane 2 : A431 cell extracts
    Lane 3 : EC-304 cell extracts


    Predicted band size : 48 kDa
    Observed band size : 48 kDa

文献

ab75747 has not yet been referenced specifically in any publications.

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