概述

  • 产品名称Anti-Cytokeratin 18抗体[DEK-18]
    参阅全部 Cytokeratin 18 一抗
  • 描述
    小鼠单克隆抗体[DEK-18] to Cytokeratin 18
  • 特异性This antibody reacts exclusively with cytokeratin 18
  • 经测试应用适用于: IHC-P, WB, IHC-Fr, ICC, Flow Cytmore details
  • 种属反应性
    与反应: Cat, Dog, Human
    不与反应: Zebrafish
  • 免疫原

    Cytoskeletal preparation extracted from the human vulvar squamous carcinoma cell line A431

  • 阳性对照
    • Cytoskeletal preparation of HaCaT cells.

性能

应用

Our Abpromise guarantee covers the use of ab9387 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/100 - 1/200. Perform enzymatic antigen retrieval before commencing with IHC staining protocol.
WB Use a concentration of 0.1 - 0.2 µg/ml.
IHC-Fr Use at an assay dependent concentration.
ICC Use at an assay dependent concentration.
Flow Cyt 1/100 - 1/200. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

靶标

  • 功能Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.
  • 组织特异性Expressed in colon, placenta, liver and very weakly in exocervix. Increased expression observed in lymph nodes of breast carcinoma.
  • 疾病相关Defects in KRT18 are a cause of cirrhosis (CIRRH) [MIM:215600].
  • 序列相似性Belongs to the intermediate filament family.
  • 翻译后修饰Phosphorylation at Ser-34 increases during mitosis. Hyperphosphorylated at Ser-53 in diseased cirrhosis liver. Phosphorylation increases by IL-6.
    Proteolytically cleaved by caspases during epithelial cell apoptosis. Cleavage occurs at Asp-238 by either caspase-3, caspase-6 or caspase-7.
    O-glycosylated at multiple sites; glycans consist of single N-acetylglucosamine residues.
  • 细胞定位Cytoplasm > perinuclear region.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Cell proliferation inducing gene 46 protein antibody
    • Cell proliferation inducing protein 46 antibody
    • Cell proliferation-inducing gene 46 protein antibody
    • CK 18 antibody
    • CK-18 antibody
    • CK18 antibody
    • CYK 18 antibody
    • CYK18 antibody
    • Cytokeratin 18 antibody
    • Cytokeratin endo B antibody
    • Cytokeratin-18 antibody
    • K 18 antibody
    • K18 antibody
    • K1C18_HUMAN antibody
    • KA18 antibody
    • Keratin 18 antibody
    • Keratin 18, type I antibody
    • Keratin D antibody
    • keratin, type I cytoskeletal 18 antibody
    • Keratin-18 antibody
    • Krt18 antibody
    see all

Anti-Cytokeratin 18 antibody [DEK-18] 图像

  • Western blot performed with ab9387 on 1.5 ul of cytoskeletal preparation from HaCaT cells.

Anti-Cytokeratin 18 antibody [DEK-18] (ab9387)参考文献

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