概述

  • 产品名称Anti-Cytokeratin 18抗体[DC10]
    参阅全部 Cytokeratin 18 一抗
  • 描述
    小鼠单克隆抗体[DC10] to Cytokeratin 18
  • 特异性We have data to indicate that this antibody may not cross react with Rat. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
  • 经测试应用适用于: WB, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Human breast cancer PMC 42 cells.

  • 阳性对照
    • HT29 cells. Skin. Breast or lung carcinoma.
  • 常规说明


    ab80572 recognizes a variety of simple epithelia including glandular epithelium but not stratified squamous epithelia.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab80572 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 48 kDa.

Use at a concentration of 1 - 2 µg/ml for 2hrs at RT.

IHC-P Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Use at a concentration of 1 - 2 µg/ml for 30 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.

ICC/IF Use at an assay dependent concentration.

靶标

  • 功能Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.
  • 组织特异性Expressed in colon, placenta, liver and very weakly in exocervix. Increased expression observed in lymph nodes of breast carcinoma.
  • 疾病相关Defects in KRT18 are a cause of cirrhosis (CIRRH) [MIM:215600].
  • 序列相似性Belongs to the intermediate filament family.
  • 翻译后修饰Phosphorylation at Ser-34 increases during mitosis. Hyperphosphorylated at Ser-53 in diseased cirrhosis liver. Phosphorylation increases by IL-6.
    Proteolytically cleaved by caspases during epithelial cell apoptosis. Cleavage occurs at Asp-238 by either caspase-3, caspase-6 or caspase-7.
    O-glycosylated at multiple sites; glycans consist of single N-acetylglucosamine residues.
  • 细胞定位Cytoplasm > perinuclear region.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Cell proliferation inducing gene 46 protein antibody
    • Cell proliferation inducing protein 46 antibody
    • Cell proliferation-inducing gene 46 protein antibody
    • CK 18 antibody
    • CK-18 antibody
    • CK18 antibody
    • CYK 18 antibody
    • CYK18 antibody
    • Cytokeratin 18 antibody
    • Cytokeratin endo B antibody
    • Cytokeratin-18 antibody
    • K 18 antibody
    • K18 antibody
    • K1C18_HUMAN antibody
    • KA18 antibody
    • Keratin 18 antibody
    • Keratin 18, type I antibody
    • Keratin D antibody
    • keratin, type I cytoskeletal 18 antibody
    • Keratin-18 antibody
    • Krt18 antibody
    see all

Anti-Cytokeratin 18 antibody [DC10] 图像

  • Formalin-fixed, paraffin-embedded human breast cancer tissue stained with ab80572 at 2µg/ml using peroxidase-conjugate and AEC chromogen. Note cytoplasmic staining of tumor cells.

Anti-Cytokeratin 18 antibody [DC10] (ab80572)参考文献

This product has been referenced in:
  • Lauerová L  et al. Novel monoclonal antibodies defining epitope of human cytokeratin 18 molecule. Hybridoma 7:495-504 (1988). Read more (PubMed: 2461901) »

See 1 Publication for this product

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