Anti-Cytokeratin 18抗体[DC 10] (ab7797)

概述

  • 产品名称
    Anti-Cytokeratin 18抗体[DC 10]
    参阅全部 Cytokeratin 18 一抗
  • 描述
    小鼠单克隆抗体[DC 10] to Cytokeratin 18
  • 特异性
    Specific reactivity for the cytokeratin 18 (45 kD) polypeptide.
  • 经测试应用
    适用于: IHC-Fr, ICC, IHC-P, Flow Cyt, ELISA, WB, IPmore details
  • 种属反应性
    与反应: Human
    不与反应: Mouse, Rat, Sheep, Hamster, Cow, Dog, Pig, a wide range of other species
  • 免疫原

    PMC-42 human breast carcinoma cells.

  • 阳性对照
    • IHC-P: Colon carcinoma WB, ELISA, IP: MCF-7 or HeLa cell lysate
  • 常规说明


    This antibody reacts with a wide variety of simple epithelia. It does not react with stratified squamous epithelia. It reacts with epithelial tumors of the gastrointestinal tract, lung, breast, pancreas, ovary, and thyroid.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    Preservative: 15mM Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • 纯度
    Protein A purified
  • Primary antibody说明
    This antibody reacts with a wide variety of simple epithelia. It does not react with stratified squamous epithelia. It reacts with epithelial tumors of the gastrointestinal tract, lung, breast, pancreas, ovary, and thyroid.
  • 克隆
    单克隆
  • 克隆编号
    DC 10
  • 同种型
    IgG1
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab7797 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr Use at an assay dependent concentration.
ICC Use at an assay dependent concentration.
IHC-P 1/10 - 1/100.
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ELISA Use at an assay dependent concentration. (Direct)
WB 1/1000.
IP Use at an assay dependent concentration.

靶标

  • 功能
    Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.
  • 组织特异性
    Expressed in colon, placenta, liver and very weakly in exocervix. Increased expression observed in lymph nodes of breast carcinoma.
  • 疾病相关
    Defects in KRT18 are a cause of cirrhosis (CIRRH) [MIM:215600].
  • 序列相似性
    Belongs to the intermediate filament family.
  • 翻译后修饰
    Phosphorylation at Ser-34 increases during mitosis. Hyperphosphorylated at Ser-53 in diseased cirrhosis liver. Phosphorylation increases by IL-6.
    Proteolytically cleaved by caspases during epithelial cell apoptosis. Cleavage occurs at Asp-238 by either caspase-3, caspase-6 or caspase-7.
    O-glycosylated at multiple sites; glycans consist of single N-acetylglucosamine residues.
  • 细胞定位
    Cytoplasm > perinuclear region.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Cell proliferation inducing gene 46 protein antibody
    • Cell proliferation inducing protein 46 antibody
    • Cell proliferation-inducing gene 46 protein antibody
    • CK 18 antibody
    • CK-18 antibody
    • CK18 antibody
    • CYK 18 antibody
    • CYK18 antibody
    • Cytokeratin 18 antibody
    • Cytokeratin endo B antibody
    • Cytokeratin-18 antibody
    • K 18 antibody
    • K18 antibody
    • K1C18_HUMAN antibody
    • KA18 antibody
    • Keratin 18 antibody
    • Keratin 18, type I antibody
    • Keratin D antibody
    • keratin, type I cytoskeletal 18 antibody
    • Keratin-18 antibody
    • Krt18 antibody
    see all

Anti-Cytokeratin 18 antibody [DC 10] 图像

  • ab7797 staining human LNCaP cells by ICC/IF.  Cells were acetone fixed and permeabilized in 0.1% Triton-X prior to blocking with 1% fish gelatin for 1 hour at 4°C.  The primary antibody was diluted 1/100 and incubated with the sample for 1 hour.  An Alexa Fluor® conjugated goat anti-mouse antibody, diluted 1/1000, was used as the secondary.

    See Abreview

  • Ab7797 staining cells from a human pancreatic cancer cell line by ICC/IF.  The cells were PFA fixed and permeabilized in 0.1% Triton X-100 prior to blocking in 1% BSA for 30 minutes at 22°C.  Ab7797 was diluted 1/100 and incubated with the sample for 1 hour at 22°C.  An Alexa Fluor® 488 conjugated donkey anti-mouse antibody was used as the secondary.

    See Abreview

  • Overlay histogram showing MCF-7 cells stained with ab7797 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab7797, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight ® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in MCF-7 cells fixed with methanol (5 min)/permeabilized in 0.1% PBS-Triton used under the same conditions.

Anti-Cytokeratin 18 antibody [DC 10] (ab7797)参考文献

This product has been referenced in:
  • Jung Y  et al. Endogenous GAS6 and Mer receptor signaling regulate prostate cancer stem cells in bone marrow. Oncotarget 7:25698-711 (2016). Read more (PubMed: 27028863) »
  • Cadieux C  et al. Mouse mammary tumor virus p75 and p110 CUX1 transgenic mice develop mammary tumors of various histologic types. Cancer Res 69:7188-97 (2009). WB ; Mouse . Read more (PubMed: 19738070) »

See all 6 Publications for this product

Product Wall

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Human retinal pigment epithelium (ARPE-19))
Permeabilization
Yes - TritonX 0,3%
Specification
Human retinal pigment epithelium (ARPE-19)
Blocking step
Serum as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
Fixative
Methanol
Username

Abcam user community

Verified customer

提交于 Jun 26 2015

Application
ELISpot
Sample
Human Recombinant protein (E coli)
Specification
E coli
Username

Abcam user community

Verified customer

提交于 May 12 2015

Application
ELISA
Sample
Human Recombinant protein (Human Cytokeratin 18 full length protein (ab73638))
Specification
Human Cytokeratin 18 full length protein (ab73638)
Type
Sandwich (Detection)
Blocking step
StartingBlock blocking buffer (Thermo Scientific) as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 100% · Temperature: 22°C
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提交于 Feb 25 2015

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Sample
Human Cell (hIPSC-derived endocrine cells)
Specification
hIPSC-derived endocrine cells
Permeabilization
Yes - 0.1 % triton x100
Fixative
Formaldehyde
Username

Dr. Youngjin Kim

Verified customer

提交于 Mar 24 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (Human breast carcinoma xenograft in mouse)
Specification
Human breast carcinoma xenograft in mouse
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
Perkin-Elmer TNB Blocking Reagent as blocking agent for 30 minute(s) · Concentration: 0.5% · Temperature: 25°C
Username

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提交于 May 01 2009

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (MDA468)
Specification
MDA468
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% Triton X-100
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 May 01 2009

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (C4-2b (prostate cancer cell line))
Specification
C4-2b (prostate cancer cell line)
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% triton
Blocking step
BSA as blocking agent for 15 minute(s) · Concentration: 0.2% · Temperature: 21°C
Username

Abcam user community

Verified customer

提交于 Jul 15 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (pancreatic cancer cell line)
Loading amount
20 µg
Specification
pancreatic cancer cell line
Gel Running Conditions
Reduced Denaturing (4-12%)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Dr. Hannah Whiteman

Verified customer

提交于 Jul 04 2008

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (pancreatic cancer cell line)
Specification
pancreatic cancer cell line
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% Triton X
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 22°C
Username

Dr. Hannah Whiteman

Verified customer

提交于 Jun 18 2008

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (LNCaP)
Specification
LNCaP
Fixative
Acetone
Permeabilization
Yes - 0.1% Triton-X
Blocking step
Fish Gelatin as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C
Username

Dr. Vivien D'Arcy

Verified customer

提交于 Mar 06 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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