Anti-Cytokeratin 18抗体[DA-7] (ab669)

概述

  • 产品名称Anti-Cytokeratin 18抗体[DA-7]
    参阅全部 Cytokeratin 18 一抗
  • 描述
    小鼠单克隆抗体[DA-7] to Cytokeratin 18
  • 特异性Human Cytokeratin peptide 18 (45 kDa).
  • 经测试应用适用于: Flow Cyt, ELISA, ICC, IHC-P, WB, IPmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Tissue/ cell preparation (Human). (Breast carcinoma cell line PMC-42).

  • 阳性对照
    • ELISA, WB, IP: MCF-7 human breast adenocarcinoma cell line lysate IHC-P: epithelial tissue (skin)

性能

应用

Our Abpromise guarantee covers the use of ab669 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use 1µg for 106 cells.
ELISA Use at an assay dependent concentration.
ICC Use at an assay dependent concentration.
IHC-P 1/10 - 1/100. Perform enzymatic antigen retrieval before commencing with IHC staining protocol.
WB 1/500. Predicted molecular weight: 45 kDa.
IP Use at an assay dependent concentration.

靶标

  • 功能Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.
  • 组织特异性Expressed in colon, placenta, liver and very weakly in exocervix. Increased expression observed in lymph nodes of breast carcinoma.
  • 疾病相关Defects in KRT18 are a cause of cirrhosis (CIRRH) [MIM:215600].
  • 序列相似性Belongs to the intermediate filament family.
  • 翻译后修饰Phosphorylation at Ser-34 increases during mitosis. Hyperphosphorylated at Ser-53 in diseased cirrhosis liver. Phosphorylation increases by IL-6.
    Proteolytically cleaved by caspases during epithelial cell apoptosis. Cleavage occurs at Asp-238 by either caspase-3, caspase-6 or caspase-7.
    O-glycosylated at multiple sites; glycans consist of single N-acetylglucosamine residues.
  • 细胞定位Cytoplasm > perinuclear region.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Cell proliferation inducing gene 46 protein antibody
    • Cell proliferation inducing protein 46 antibody
    • Cell proliferation-inducing gene 46 protein antibody
    • CK 18 antibody
    • CK-18 antibody
    • CK18 antibody
    • CYK 18 antibody
    • CYK18 antibody
    • Cytokeratin 18 antibody
    • Cytokeratin endo B antibody
    • Cytokeratin-18 antibody
    • K 18 antibody
    • K18 antibody
    • K1C18_HUMAN antibody
    • KA18 antibody
    • Keratin 18 antibody
    • Keratin 18, type I antibody
    • Keratin D antibody
    • keratin, type I cytoskeletal 18 antibody
    • Keratin-18 antibody
    • Krt18 antibody
    see all

Anti-Cytokeratin 18 antibody [DA-7] 图像

  • Ab669 staining Human normal skin. Staining is localized to the cytoplasm.
    Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control.
    Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffers EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
  • Overlay histogram showing MCF7 cells stained with ab669 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab669, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in MCF7 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-Cytokeratin 18 antibody [DA-7] (ab669)参考文献

This product has been referenced in:
  • Kovarik J  et al. J. Tumor Marker Oncol. 5:219 (1990). Read more (PubMed: ) »
  • Taylor-Papadimitriou J  et al. Keratin expression in human mammary epithelial cells cultured from normal and malignant tissue: relation to in vivo phenotypes and influence of medium. J Cell Sci 94 ( Pt 3):403-13 (1989). Read more (PubMed: 2483723) »

See all 5 Publications for this product

Product Wall

The epitope has not been determined.

I enquired with the supplier of ab669 and ab7797 and unfortunately they do not have a specific ELISA protocol for either of these two antibodies. All we can recommend is to consult Abcam's general ELISA protocol. If you have any more questions or conce...

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