特异性The antibody reacts specifically with a wide variety of simple epithelia (e.g. intestine, respiratory and urinary systems, liver, and glandular epithelia). It does not react with stratified squamous epithelia (e.g. esophagus or epidermis) or with non-epithelial cells.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
Use at an assay dependent concentration. Predicted molecular weight: 48 kDa.
功能Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.
组织特异性Expressed in colon, placenta, liver and very weakly in exocervix. Increased expression observed in lymph nodes of breast carcinoma.
疾病相关Defects in KRT18 are a cause of cirrhosis (CIRRH) [MIM:215600].
序列相似性Belongs to the intermediate filament family.
翻译后修饰Phosphorylation at Ser-34 increases during mitosis. Hyperphosphorylated at Ser-53 in diseased cirrhosis liver. Phosphorylation increases by IL-6. Proteolytically cleaved by caspases during epithelial cell apoptosis. Cleavage occurs at Asp-238 by either caspase-3, caspase-6 or caspase-7. O-glycosylated at multiple sites; glycans consist of single N-acetylglucosamine residues.
Cell proliferation inducing gene 46 protein antibody
Cell proliferation inducing protein 46 antibody
Cell proliferation-inducing gene 46 protein antibody
CK 18 antibody
CYK 18 antibody
Cytokeratin 18 antibody
Cytokeratin endo B antibody
K 18 antibody
Keratin 18 antibody
Keratin 18, type I antibody
Keratin D antibody
keratin, type I cytoskeletal 18 antibody
Anti-Cytokeratin 18 antibody [CY-90] 图像
Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 18 antibody [CY-90] (ab49824)Image from Moore RN et al., Stem Cell Res., 3(1), 51-62. Fig 6.; doi: 10.1016/j.scr.2009.04.004 with permission from Elsevier.
ab49824 staining Cytokeratin 18 (green) in Human hESC-derived cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% paraformaldehyde and permeabilized with SAP buffer (0.5% saponin, 1% BSA, 0.1% sodium azide) for 30 minutes at room temperature. Cells were blocked with 3% BSA for 30 minutes at room temperature. Samples were incubated with primary antibody (1/800 in SAP buffer) overnight at 4°C. An Alexa Fluor®488-conjugated anti-mouse IgG1 (1/1000) was used as the secondary antibody. Blue - DAPI, Red - albumin
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