Anti-Cytokeratin 18抗体[CK-18] (ab82254)
- 数据表
- 具体的参考文献 (2)
- 实验方案
概述
- 产品名称Anti-Cytokeratin 18抗体[CK-18]
参阅全部 Cytokeratin 18 一抗 - 描述小鼠单克隆抗体[CK-18] to Cytokeratin 18
- 特异性No cross reactivity with other proteins.
- 经测试应用适用于: Flow Cyt, WB, IHC-P, ICC/IF, IHC-Fr, IPmore details
- 种属反应性与反应: Rat, Human
- 免疫原
Tissue, cells or virus corresponding to Human Cytokeratin 18. Human epidermal carcinoma A431 and MCF7 human breast cancer cell lines.
- 阳性对照
- This antibody gave a positive signal in HCT116 cell line in IF/ICC.
性能
- 形式Liquid
- 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
- 存储溶液Preservative: 0.01% Sodium azide
Constituents: 1.2% Sodium acetate, 2% BSA -
Concentration information loading... - 纯度Ascites
- 纯化说明ab82254 is purified using anti-mouse IgG affinity chromatography.
- 克隆单克隆
- 克隆编号CK-18
- 同种型IgG1
- 研究领域
相关产品
- Compatible Secondaries
- Isotype control
- Related Products
应用
Our Abpromise guarantee covers the use of ab82254 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt | Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. | |
| WB | Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 48 kDa. | |
| IHC-P | Use a concentration of 1 - 2 µg/ml. | |
| ICC/IF | Use a concentration of 1 µg/ml. | |
| IHC-Fr | Use a concentration of 0.5 - 2 µg/ml. Use formalin or acetone fixed tissues. | |
| IP | Use a concentration of 5 µg/ml. |
靶标
- 功能Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.
- 组织特异性Expressed in colon, placenta, liver and very weakly in exocervix. Increased expression observed in lymph nodes of breast carcinoma.
- 疾病相关Defects in KRT18 are a cause of cirrhosis (CIRRH) [MIM:215600].
- 序列相似性Belongs to the intermediate filament family.
- 翻译后修饰Phosphorylation at Ser-34 increases during mitosis. Hyperphosphorylated at Ser-53 in diseased cirrhosis liver. Phosphorylation increases by IL-6.
Proteolytically cleaved by caspases during epithelial cell apoptosis. Cleavage occurs at Asp-238 by either caspase-3, caspase-6 or caspase-7.
O-glycosylated at multiple sites; glycans consist of single N-acetylglucosamine residues. - 细胞定位Cytoplasm > perinuclear region.
- Information by UniProt
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数据库链接
- Entrez Gene: 3875 Human
- Entrez Gene: 294853 Rat
- Omim: 148070 Human
- SwissProt: P05783 Human
- SwissProt: Q5BJY9 Rat
- Unigene: 406013 Human
- Unigene: 103924 Rat
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别名
- Cell proliferation inducing gene 46 protein antibody
- Cell proliferation inducing protein 46 antibody
- Cell proliferation-inducing gene 46 protein antibody
see all
Anti-Cytokeratin 18 antibody [CK-18] 图像
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![Western blot - Cytokeratin 18 antibody [CK-18] (ab82254)](http://www.abcam.cn/ps/datasheet/images/82/ab82254/Cytokeratin-18-Primary-antibodies-ab82254-1.JPG)
Western blot - Cytokeratin 18 antibody [CK-18] (ab82254)Anti-Cytokeratin 18 antibody [CK-18] (ab82254) + MCF-7 whole cell lysate
Predicted band size : 48 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Cytokeratin 18 antibody [CK-18] (ab82254)](http://www.abcam.cn/ps/datasheet/images/82/ab82254/Cytokeratin-18-Primary-antibodies-ab82254-2.JPG)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Cytokeratin 18 antibody [CK-18] (ab82254)ab82254 staining Cytokeratin 18 in paraffin-embedded human rectal cancer tissue. -
![Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 18 antibody [CK-18] (ab82254)](http://www.abcam.cn/ps/datasheet/images/82/ab82254/Cytokeratin-18-Primary-antibodies-ab82254-3.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 18 antibody [CK-18] (ab82254)ICC/IF image of ab82254 stained HCT116 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab82254, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. -
](http://www.abcam.cn/ps/datasheet/images/82/ab82254/Cytokeratin-18-Primary-antibodies-ab82254-4.jpg)
Flow Cytometry-Anti-Cytokeratin 18 antibody [CK-18](ab82254)Overlay histogram showing MCF7 cells stained with ab82254 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab82254, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in MCF7 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions. -
![Immunoprecipitation - Anti-Cytokeratin 18 antibody [CK-18] (ab82254)](http://www.abcam.cn/ps/datasheet/images/82/ab82254/Cytokeratin-18-Primary-antibodies-ab82254-6.jpg)
Immunoprecipitation - Anti-Cytokeratin 18 antibody [CK-18] (ab82254)Cytokeratin 18 was immunoprecipitated using 0.5mg MCF7 Whole cell lysate, 5µg of Mouse monoclonal to Cytokeratin 18 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, MCF7 Whole cell lysate lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab82254.
Secondary: Protein G-HRP at 1/500 dilution.
Band: 45kDa; Cytokeratin 18: non specific bands - 49kDa: We are unsure as to the identity of this extra band.
Anti-Cytokeratin 18 antibody [CK-18] (ab82254)参考文献
This product has been referenced in:
- Powell E et al. p53 deficiency linked to B cell translocation gene 2 (BTG2) loss enhances metastatic potential by promoting tumor growth in primary and metastatic sites in patient-derived xenograft (PDX) models of triple-negative breast cancer. Breast Cancer Res 18:13 (2016). IHC ; Human . Read more (PubMed: 26818199) »
- Kruithof-de Julio M et al. Canonical Wnt signaling regulates Nkx3.1 expression and luminal epithelial differentiation during prostate organogenesis. Dev Dyn 242:1160-71 (2013). Read more (PubMed: 23813564) »
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