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Tissue, cells or virus corresponding to Human Cytokeratin 18. Human epidermal carcinoma A431 and MCF7 human breast cancer cell lines.
Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. We would recommend antibody ab133263 as a replacement.
Our Abpromise guarantee covers the use of ab82254 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|WB||Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 48 kDa.|
|IHC-P||Use a concentration of 1 - 2 µg/ml.|
|ICC/IF||Use a concentration of 1 µg/ml.|
|IHC-Fr||Use a concentration of 0.5 - 2 µg/ml. Use formalin or acetone fixed tissues.|
|IP||Use a concentration of 5 µg/ml.|
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"