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Our Abpromise guarantee covers the use of ab27553 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration.|
|WB||1/500. Predicted molecular weight: 47 kDa.|
|ICC/IF||Use at an assay dependent concentration. PubMed: 18556507|
ab27553 at 1/100 staining mouse ES cell derived hepatocyte-like cells by ICC/IF. The cells were formaldehyde fixed and permeabilized with Triton X100 before being blocked with BSA and then incubated with the antibody for 18 hoursat 4°C.
ab27553 staining Cytokeratin 18 in Mouse mammary gland cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed in 1:1 Acetone:Methanol and permeabilized with 0.025% Triton X-100 prior to blocking in 10% serum for 1 hour at 25°C. The primary antibody was applied at a concentration of 2 µg/mL and incubated with the sample for 15 hours at 4°C. The secondary antibody was Streptavidin-Cy3®-conjugated Mouse monoclonal, applied at a concentration of 1 µg/mL.
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