Anti-Cytokeratin 18抗体(ab52948)


  • 产品名称Anti-Cytokeratin 18抗体
    参阅全部 Cytokeratin 18 一抗
  • 描述
    兔多克隆抗体to Cytokeratin 18
  • 经测试应用适用于: WB, IP, Flow Cyt, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse
  • 免疫原

    A synthetic peptide corresponding to residues on the C-terminus of human Cytokeratin 18

  • 阳性对照
    • A431 cell lysate, human urothelial carcinoma cells and HeLa cells.



Our Abpromise guarantee covers the use of ab52948 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/10000 - 1/50000. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).
IP 1/100.
Flow Cyt 1/15. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
IHC-P Use at an assay dependent concentration.
ICC/IF 1/250 - 1/500.


  • 功能Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.
  • 组织特异性Expressed in colon, placenta, liver and very weakly in exocervix. Increased expression observed in lymph nodes of breast carcinoma.
  • 疾病相关Defects in KRT18 are a cause of cirrhosis (CIRRH) [MIM:215600].
  • 序列相似性Belongs to the intermediate filament family.
  • 翻译后修饰Phosphorylation at Ser-34 increases during mitosis. Hyperphosphorylated at Ser-53 in diseased cirrhosis liver. Phosphorylation increases by IL-6.
    Proteolytically cleaved by caspases during epithelial cell apoptosis. Cleavage occurs at Asp-238 by either caspase-3, caspase-6 or caspase-7.
    O-glycosylated at multiple sites; glycans consist of single N-acetylglucosamine residues.
  • 细胞定位Cytoplasm > perinuclear region.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Cell proliferation inducing gene 46 protein antibody
    • Cell proliferation inducing protein 46 antibody
    • Cell proliferation-inducing gene 46 protein antibody
    • CK 18 antibody
    • CK-18 antibody
    • CK18 antibody
    • CYK 18 antibody
    • CYK18 antibody
    • Cytokeratin 18 antibody
    • Cytokeratin endo B antibody
    • Cytokeratin-18 antibody
    • K 18 antibody
    • K18 antibody
    • K1C18_HUMAN antibody
    • KA18 antibody
    • Keratin 18 antibody
    • Keratin 18, type I antibody
    • Keratin D antibody
    • keratin, type I cytoskeletal 18 antibody
    • Keratin-18 antibody
    • Krt18 antibody
    see all

Anti-Cytokeratin 18 antibody 图像

  • Anti-Cytokeratin 18 antibody (ab52948) at 1/50000 dilution + A431 cell lysate at 10 µg

    goat anti-rabbit HR at 1/2000 dilution

    Predicted band size : 48 kDa
    Observed band size : 48 kDa
  • Anti-Cytokeratin 18 antibody (ab52948) at 1/250 dilution + Recombinant Human Cytokeratin 18 protein (ab73638) at 0.01 µg

    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 1 minute
  • ab52948 at 1/250 dilution staining Cytokeratin 18 in urothelial carcinoma cells by Immunohistochemistry, Paraffin embedded tissue.
  • ab52948 at 1/250 dilution staining Cytokeratin 18 in HeLa cells by Immunofluorescence

Anti-Cytokeratin 18 antibody (ab52948)参考文献

This product has been referenced in:
  • Joiner AM  et al. Primary Cilia on Horizontal Basal Cells Regulate Regeneration of the Olfactory Epithelium. J Neurosci 35:13761-72 (2015). IHC ; Mouse . Read more (PubMed: 26446227) »
  • Chung CY  et al. Progressive Proximal-to-Distal Reduction in Expression of the Tight Junction Complex in Colonic Epithelium of Virally-Suppressed HIV+ Individuals. PLoS Pathog 10:e1004198 (2014). WB ; Human . Read more (PubMed: 24968145) »

See all 7 Publications for this product

Product Wall

Application ELISA
Sample Human Recombinant protein (Human Cytokeratin 18 full length protein (ab73638))
Specification Human Cytokeratin 18 full length protein (ab73638)
Type Sandwich (Capture)
Blocking step StartingBlock blocking buffer (Thermo Scientific) as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 100% · Temperature: 22°C

Abcam user community

Verified customer

提交于 Feb 25 2015

The cat number is ab133272 and this one has been used for WB and IP.

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