Anti-Cytokeratin 18抗体(ab52948)


  • 产品名称
    Anti-Cytokeratin 18抗体
    参阅全部 Cytokeratin 18 一抗
  • 描述
    兔多克隆抗体to Cytokeratin 18
  • 宿主
  • 经测试应用
    适用于: WB, IP, Flow Cyt, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse
  • 免疫原

    Synthetic peptide within Human Cytokeratin 18 aa 400 to the C-terminus (C terminal). The exact sequence is proprietary.
    (Peptide available as ab215576)

  • 阳性对照
    • A431 cell lysate, human urothelial carcinoma cells and HeLa cells.



Our Abpromise guarantee covers the use of ab52948 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/10000 - 1/50000. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).
IP 1/100.
Flow Cyt 1/15.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

IHC-P Use at an assay dependent concentration.
ICC/IF 1/250 - 1/500.


  • 功能
    Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.
  • 组织特异性
    Expressed in colon, placenta, liver and very weakly in exocervix. Increased expression observed in lymph nodes of breast carcinoma.
  • 疾病相关
    Defects in KRT18 are a cause of cirrhosis (CIRRH) [MIM:215600].
  • 序列相似性
    Belongs to the intermediate filament family.
  • 翻译后修饰
    Phosphorylation at Ser-34 increases during mitosis. Hyperphosphorylated at Ser-53 in diseased cirrhosis liver. Phosphorylation increases by IL-6.
    Proteolytically cleaved by caspases during epithelial cell apoptosis. Cleavage occurs at Asp-238 by either caspase-3, caspase-6 or caspase-7.
    O-glycosylated at multiple sites; glycans consist of single N-acetylglucosamine residues.
  • 细胞定位
    Cytoplasm > perinuclear region.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Cell proliferation inducing gene 46 protein antibody
    • Cell proliferation inducing protein 46 antibody
    • Cell proliferation-inducing gene 46 protein antibody
    • CK 18 antibody
    • CK-18 antibody
    • CK18 antibody
    • CYK 18 antibody
    • CYK18 antibody
    • Cytokeratin 18 antibody
    • Cytokeratin endo B antibody
    • Cytokeratin-18 antibody
    • K 18 antibody
    • K18 antibody
    • K1C18_HUMAN antibody
    • KA18 antibody
    • Keratin 18 antibody
    • Keratin 18, type I antibody
    • Keratin D antibody
    • keratin, type I cytoskeletal 18 antibody
    • Keratin-18 antibody
    • Krt18 antibody
    see all


  • Anti-Cytokeratin 18 antibody (ab52948) at 1/50000 dilution + A431 cell lysate at 10 µg

    goat anti-rabbit HR at 1/2000 dilution

    Predicted band size: 48 kDa
    Observed band size: 48 kDa

  • ab52948 at 1/250 dilution staining Cytokeratin 18 in urothelial carcinoma cells by Immunohistochemistry, Paraffin embedded tissue.
  • ab52948 at 1/250 dilution staining Cytokeratin 18 in HeLa cells by Immunofluorescence


This product has been referenced in:
  • De Cian MC  et al. Amplification of R-spondin1 signaling induces granulosa cell fate defects and cancers in mouse adult ovary. Oncogene 36:208-218 (2017). IHC-P ; Mouse . Read more (PubMed: 27270435) »
  • Di Lullo AM  et al. An "ex vivo model" contributing to the diagnosis and evaluation of new drugs in cystic fibrosis. Acta Otorhinolaryngol Ital 37:207-213 (2017). ICC ; Human . Read more (PubMed: 27897275) »

See all 14 Publications for this product


Human Recombinant protein (Human Cytokeratin 18 full length protein (ab73638))
Human Cytokeratin 18 full length protein (ab73638)
Sandwich (Capture)
Blocking step
StartingBlock blocking buffer (Thermo Scientific) as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 100% · Temperature: 22°C

Abcam user community

Verified customer

提交于 Feb 25 2015

The cat number is ab133272 and this one has been used for WB and IP.

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