RabMAb

Anti-Cytokeratin 16抗体[EP1615Y] (ab76416)

概述

  • 产品名称Anti-Cytokeratin 16抗体[EP1615Y]
    参阅全部 Cytokeratin 16 一抗
  • 描述
    兔单克隆抗体[EP1615Y] to Cytokeratin 16
  • 经测试应用适用于: IHC-P, WB, Flow Cyt, ICC/IFmore details
    不适用于: IP
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide. within Human Cytokeratin 16 aa 1-100 (N terminal). The exact sequence is proprietary.
    Database link: P08779

  • 阳性对照
    • HaCat cell lysate; human squamous cervical carcinoma tissue.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

     

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Alternative versions available:

    Anti-Cytokeratin 16 antibody (Alexa Fluor® 647) [EP1615Y] (ab205745)

    Anti-Cytokeratin 16 antibody (Alexa Fluor® 488) [EP1615Y] (ab205744)

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

性能

应用

Our Abpromise guarantee covers the use of ab76416 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
WB 1/5000 - 1/10000. Predicted molecular weight: 51 kDa.
Flow Cyt 1/50 - 1/150.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF 1/100 - 1/150.
  • 应用说明Is unsuitable for IP.
  • 靶标

    • 组织特异性Expressed in the hair follicle, nail bed and in mucosal stratified squamous epithelia and, suprabasally, in oral epithelium and palmoplantar epidermis. Also found in luminal cells of sweat and mammary gland ducts.
    • 疾病相关Defects in KRT16 are a cause of pachyonychia congenita type 1 (PC1) [MIM:167200]; also known as Jadassohn-Lewandowsky syndrome. PC1 is an autosomal dominant ectodermal dysplasia characterized by hypertrophic nail dystrophy resulting in onchyogryposis (thickening and increase in curvature of the nail), palmoplantar keratoderma, follicular hyperkeratosis, and oral leukokeratosis. Hyperhidrosis of the hands and feet is usually present.
      Defects in KRT16 are the cause of palmoplantar keratoderma non-epidermolytic focal (FNEPPK) [MIM:613000]. A dermatological disorder characterized by non-epidermolytic palmoplantar keratoderma limited to the pressure points on the balls of the feet, with later mild involvement on the palms. Oral, genital and follicular keratotic lesions are often present.
      Defects in KRT16 are a cause of unilateral palmoplantar verrucous nevus (UPVN) [MIM:144200]. UPVN is characterized by a localized thickening of the skin in parts of the right palm and the right sole.
      Note=KRT16 and KRT17 are coexpressed only in pathological situations such as metaplasias and carcinomas of the uterine cervix and in psoriasis vulgaris.
    • 序列相似性Belongs to the intermediate filament family.
    • Information by UniProt
    • 数据库链接
    • 别名
      • CK 16 antibody
      • CK-16 antibody
      • CK16 antibody
      • Cytokeratin-16 antibody
      • Cytokeratin16 antibody
      • FNEPPK antibody
      • Focal non epidermolytic palmoplantar keratoderma antibody
      • K 16 antibody
      • K16 antibody
      • K1C16_HUMAN antibody
      • K1CP antibody
      • Keratin 1 type I antibody
      • Keratin 16 antibody
      • Keratin antibody
      • Keratin type I cytoskeletal 16 antibody
      • Keratin-16 antibody
      • Keratin16 antibody
      • KRT 16 antibody
      • Krt16 antibody
      • KRT16A antibody
      • NEPPK antibody
      • PC1 antibody
      • type I cytoskeletal 16 antibody
      see all

    Anti-Cytokeratin 16 antibody [EP1615Y] 图像

    • Anti-Cytokeratin 16 antibody [EP1615Y] (ab76416) at 1/10000 dilution + HACAT (human keratinocyte) whole cell lysate at 10 µg

      Secondary
      Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051 at 1/20000 dilution

      Predicted band size : 51 kDa
    • ab76416 staining Cytokeratin 16 in A431 (human epidermoid carcinoma) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody at a dilution of 1/100. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody. ab7291 and ab150120 were used as counterstains for primary antibody ab75748 and secondary antibody ab150077 respectively and DAPI was used as a nuclear counterstain.

      Negative control 1: Rabbit primary antibody and anti-mouse secondary antibody (ab150120)
      Negative control 2: Mouse primary antibody (ab7291) and anti-rabbit secondary antibody (ab150077)

       

    • ab76416 staining Cytokeratin 16 in human skin tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/500. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at a dilution of 1/500.

      Negative control 1: PBS in place of primary antibody.

       

    • Overlay histogram showing HepG2 cells stained with unpurified ab76416 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Triton for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab76416, 1/50 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit monoclonal IgG (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 4% paraformaldehyde/permeabilized in 0.1% PBS-Triton used under the same conditions.

    • ab76416 staining Cytokeratin 16 in HACAT (human keratinocyte) cell line by flow cytometry. Cells were fixed with 4% paraformaldehyde and the sample was incubated with the primary antibody at a dilution of 1/150. A goat anti rabbit IgG (Alexa Fluor® 488) at a dilution of 1/500 was used as the secondary antibody.

      Isoytype control: Rabbit monoclonal IgG (Black)

      Unlabelled control: Cell without incubation with primary antibody and secondary antibody (Blue)

       

    • ab76416 staining Cytokeratin 16 in human skeletal muscle sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/500. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at a dilution of 1/500.

      Negative control 1: PBS in place of primary antibody.

    Anti-Cytokeratin 16 antibody [EP1615Y] (ab76416)参考文献

    This product has been referenced in:
    • Khanom R  et al. Keratin 17 Is Induced in Oral Cancer and Facilitates Tumor Growth. PLoS One 11:e0161163 (2016). Read more (PubMed: 27512993) »
    • Pal SK  et al. The expression profiles of acidic epithelial keratins in ameloblastoma. Oral Surg Oral Med Oral Pathol Oral Radiol 115:523-31 (2013). IHC-P ; Human . Read more (PubMed: 23522646) »

    See all 9 Publications for this product

    Product Wall

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Human Tissue sections (Human Psoriasis)
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Dako high pH buffer
    Permeabilization No
    Specification Human Psoriasis
    Blocking step Dako Flex peroxidase block as blocking agent for 5 minute(s) · Concentration: 100% · Temperature: RT°C
    Fixative Paraformaldehyde
    Username

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    提交于 Nov 24 2015

    This product ab76416 is the primary antibody Anti-Cytokeratin 16 antibody [EP1615Y] only.

    The positive control therefore does not automatically come with this product.

    We have listed HaCat cell lysate as a positive control on our da...

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    Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

    I am sorry these products did not perform as stated on the datasheet and for the inconvenience th...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"