Anti-Cytokeratin 15抗体[LHK15] (ab80522)


  • 产品名称Anti-Cytokeratin 15抗体[LHK15]
    参阅全部 Cytokeratin 15 一抗
  • 描述
    小鼠单克隆抗体[LHK15] to Cytokeratin 15
  • 经测试应用适用于: ICC/IF, IHC-P, Flow Cytmore details
  • 种属反应性
    与反应: Mouse, Rat, Cow, Human
  • 免疫原

    17 amino acids synthetic peptide derived from the C terminal region of Human Cytokeratin 15



Our Abpromise guarantee covers the use of ab80522 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
Flow Cyt Use 1µg for 106 cells.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.


  • 组织特异性Expressed in a discontinuous manner in the basal cell layer of adult skin epidermis, but continuously in the basal layer of fetal skin epidermis and nail. Also expressed in the outer root sheath above the hair bulb in hair follicle (at protein level). Expressed homogeneously in all cell layers of the esophagus and exocervix, but detected in the basal cell layer only of oral mucosa, skin and in the basal plus the next two layers of the suprabasal epithelium of the palate.
  • 序列相似性Belongs to the intermediate filament family.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AI528832 antibody
    • CK 15 antibody
    • CK-15 antibody
    • CK15 antibody
    • Cytokeratin-15 antibody
    • Cytokeratin15 antibody
    • K15 antibody
    • K1C15_HUMAN antibody
    • K1CO antibody
    • Ka15 antibody
    • Keratin 15 antibody
    • Keratin 15 basic antibody
    • Keratin 15 beta antibody
    • Keratin antibody
    • Keratin complex 1 acidic gene 15 antibody
    • Keratin type I cytoskeletal 15 antibody
    • Keratin-15 antibody
    • Keratin15 antibody
    • KRT 15 antibody
    • Krt1-15 antibody
    • KRT15 antibody
    • KRTB antibody
    • KRTL15 antibody
    • Type I cytoskeletal 15 antibody
    • Type I keratin Ka15 antibody
    see all

Anti-Cytokeratin 15 antibody [LHK15] 图像

  • ab80522 staining Cytokeratin 15 in mouse colon tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde; antigen retrieval was by heat mediation. Samples were incubated with primary antibody (undiluted) for 10 hours.

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  • ab8969 staining keratin 15 in rat hair folicles by immunocytochemistry/ immunofluorescence. Cells were paraformaldehyde fixed and permeabilized in 0.25% Triton X-100 prior to blocking in 1% BSA for 10 minutes at 21°C. The primary antibody was diluted 1/100 and incubated with the sample for 1 hour at 21°C. A TRITC conjugated mouse anti-mouse antibody, diluted 1/85, was used as the secondary.

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  • Overlay histogram showing A431 cells stained with ab80522 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab80522, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

Anti-Cytokeratin 15 antibody [LHK15] (ab80522)参考文献

This product has been referenced in:
  • Soady KJ  et al. Mouse mammary stem cells express prognostic markers for triple-negative breast cancer. Breast Cancer Res 17:31 (2015). ICC/IF ; Mouse . Read more (PubMed: 25849541) »
  • Waseem A  et al. Keratin 15 expression in stratified epithelia: downregulation in activated keratinocytes. J Invest Dermatol 112:362-9 (1999). WB, ICC/IF ; Human . Read more (PubMed: 10084315) »

See all 2 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Antigen retrieval step Heat mediated
Sample Mouse Tissue sections (Colon)
Specification Colon
Blocking step (agent) for 1 hour(s) and 0 minute(s) · Concentration: 5%
Fixative Formaldehyde

Abcam user community

Verified customer

提交于 Dec 23 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cell (hair follicle, HR)
Specification hair follicle, HR
Fixative Paraformaldehyde
Permeabilization Yes - 0.25% Triton X-100
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C

Mr. Maciej Gagat

Verified customer

提交于 Aug 28 2009