Anti-Cytokeratin 14抗体[LL002] (ab7800)

概述

  • 产品名称Anti-Cytokeratin 14抗体[LL002]
    参阅全部 Cytokeratin 14 一抗
  • 描述
    小鼠单克隆抗体[LL002] to Cytokeratin 14
  • 经测试应用适用于: WB, Flow Cyt, ICC/IF, IHC-FoFr, IHC-P, IHC-Frmore details
  • 种属反应性
    与反应: Mouse, Horse, Human
  • 免疫原

    Thyroglobulin conjugated synthetic pentadecapeptide of extreme carboxyl-terminal of human cytokeratin 14.

  • 常规说明


    This antibody labels the basal layer of stratifying squamous and non-squamous epithelia. The staining pattern iscytoplasmic. It recognizes basal cell carcinomas and squamous cell carcinomas.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.1% Sodium Azide
    Constituents: 20mM Tris, 150mM Sodium chloride
  • 纯度Tissue culture supernatant
  • Primary antibody说明This antibody labels the basal layer of stratifying squamous and non-squamous epithelia. The staining pattern iscytoplasmic. It recognizes basal cell carcinomas and squamous cell carcinomas.
  • 克隆单克隆
  • 克隆编号LL002
  • 同种型IgG3
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab7800 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent concentration.
Flow Cyt 1/50.

ab91537 - Mouse monoclonal IgG3, is suitable for use as an isotype control with this antibody.

ICC/IF 1/200.
IHC-FoFr Use at an assay dependent concentration.
IHC-P 1/100 - 1/400. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. (See abreview)
IHC-Fr 1/20 - 1/300. PubMed: 23769181

靶标

  • 功能The nonhelical tail domain is involved in promoting KRT5-KRT14 filaments to self-organize into large bundles and enhances the mechanical properties involved in resilience of keratin intermediate filaments in vitro.
  • 组织特异性Detected in the basal layer, lowered within the more apically located layers specifically in the stratum spinosum, stratum granulosum but is not detected in stratum corneum. Strongly expressed in the outer root sheath of anagen follicles but not in the germinative matrix, inner root sheath or hair. Found in keratinocytes surrounding the club hair during telogen.
  • 疾病相关Defects in KRT14 are a cause of epidermolysis bullosa simplex Dowling-Meara type (DM-EBS) [MIM:131760]. DM-EBS is a severe form of intraepidermal epidermolysis bullosa characterized by generalized herpetiform blistering, milia formation, dystrophic nails, and mucous membrane involvement.
    Defects in KRT14 are a cause of epidermolysis bullosa simplex Weber-Cockayne type (WC-EBS) [MIM:131800]. WC-EBS is a form of intraepidermal epidermolysis bullosa characterized by blistering limited to palmar and plantar areas of the skin.
    Defects in KRT14 are a cause of epidermolysis bullosa simplex Koebner type (K-EBS) [MIM:131900]. K-EBS is a form of intraepidermal epidermolysis bullosa characterized by generalized skin blistering. The phenotype is not fundamentally distinct from the Dowling-Meara type, although it is less severe.
    Defects in KRT14 are the cause of epidermolysis bullosa simplex autosomal recessive (AREBS) [MIM:601001]. AREBS is an intraepidermal epidermolysis bullosa characterized by localized blistering on the dorsal, lateral and plantar surfaces of the feet.
    Defects in KRT14 are the cause of Naegeli-Franceschetti-Jadassohn syndrome (NFJS) [MIM:161000]; also known as Naegeli syndrome. NFJS is a rare autosomal dominant form of ectodermal dysplasia. The cardinal features are absence of dermatoglyphics (fingerprints), reticular cutaneous hyperpigmentation (starting at about the age of 2 years without a preceding inflammatory stage), palmoplantar keratoderma, hypohidrosis with diminished sweat gland function and discomfort provoked by heat, nail dystrophy, and tooth enamel defects.
    Defects in KRT14 are the cause of dermatopathia pigmentosa reticularis (DPR) [MIM:125595]. DPR is a rare ectodermal dysplasia characterized by lifelong persistent reticulate hyperpigmentation, noncicatricial alopecia, and nail dystrophy.
  • 序列相似性Belongs to the intermediate filament family.
  • 细胞定位Cytoplasm. Nucleus. Expressed in both as a filamentous pattern.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CK 14 antibody
    • CK-14 antibody
    • ck14 antibody
    • Cytokeratin 14 antibody
    • Cytokeratin-14 antibody
    • Cytokeratin14 antibody
    • Dowling Meara antibody
    • EBS3 antibody
    • EBS4 antibody
    • Epidermolysis bullosa simplex antibody
    • K14 antibody
    • K1C14_HUMAN antibody
    • Keratin 14 (epidermolysis bullosa simplex, Dowling-Meara, Koebner) antibody
    • Keratin 14 antibody
    • Keratin antibody
    • Keratin type I cytoskeletal 14 antibody
    • Keratin, type I cytoskeletal 14 antibody
    • Keratin-14 antibody
    • Keratin14 antibody
    • Koebner antibody
    • Krt 14 antibody
    • Krt14 antibody
    • NFJ antibody
    • OTTHUMP00000164624 antibody
    • type I cytoskeletal 14 antibody
    see all

Anti-Cytokeratin 14 antibody [LL002] 图像

  • ab7800 was used to stain mouse prostate.

  • Immunohistochemical analysis of mouse mammary duct tissue, staining Cytokeratin 14 (red) with ab7800.

    Antigen retrieval was carried out on paraffin-embedded sections by boiling in citrate buffer (pH 6) for 18 minutes in a microwave. Sections were then blocked for 1.5 hours in blocking reagents, before incubating with primary antibody (0.26 µg/ml) overnight at 4°C. An AlexaFluor®555-conjugated goat anti-mouse IgG was used as the secondary antibody

  • Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 10 seconds
  • ab7800 staining Cytokeratin 14 in Human normal skin tissue sections by IHC-P (Formaldehyde-fixed, Paraffin-embedded sections). Tissue samples were fixed with formaldehyde and blocked with 10% Serum for 30 minutes at 21°C; antigen retrieval was by heat mediation in citrate buffer (pH 6). The sample was incubated with primary antibody (1/100 in PBS + 0.5% Tween-20 + 0.5% BSA)) at 21°C for 30 minutes. An undiluted HRP-conjugated goat polyclonal to mouse IgG was used as secondary antibody.

    See Abreview

  • Overlay histogram showing A431 cells stained with ab7800 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab7800, 1/50 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG3 [MG3-35] (ab18394, 2µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in A431 cells fixed with 100% methanol (5 min)/permeabilized in 0.1% PBS-Triton used under the same conditions.

  • Anti-Cytokeratin 14 antibody [LL002] (ab7800) at 2 µg/ml + Human HaCaT whole cell lysate at 30 µg

    Secondary
    Goat Anti-mouse IgG Polyclonal at 1/20000 dilution
    Developed using the ECL technique

    Observed band size : 55 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 minute

    This image is courtesy of an anonymous Abreview

    Blocking Step: 5% Milk for 12 hours at 4°C
    Gel Running Conditions: 15%,6V,50min; Reduced; Denaturing

    See Abreview

  • Immunofluorescence analysis of mouse mammary epithelial cells, staining Cytokeratin 14 (red) with ab7800.

Anti-Cytokeratin 14 antibody [LL002] (ab7800)参考文献

This product has been referenced in:
  • Liu Y  et al. Fbxo30 Regulates Mammopoiesis by Targeting the Bipolar Mitotic Kinesin Eg5. Cell Rep 15:1111-22 (2016). IHC-P ; Mouse . Read more (PubMed: 27117404) »
  • Smirnova NF  et al. Detection and quantification of epithelial progenitor cell populations in human healthy and IPF lungs. Respir Res 17:83 (2016). IHC-P ; Human . Read more (PubMed: 27423691) »

See all 36 Publications for this product

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (Human prostate cancer cell)
Gel Running Conditions Reduced Denaturing
Loading amount 30 µg
Specification Human prostate cancer cell
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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提交于 Aug 18 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Mammary gland)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 1mM EDTA 95ºC pH=8.0
Permeabilization Yes - PBS with 3% Triton
Specification Mammary gland
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative Formaldehyde
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提交于 Aug 04 2015

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing
Sample Human Cell lysate - whole cell (Breast -MCF7)
Specification Breast -MCF7
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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提交于 Jan 07 2015

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Sample Human Cell (Breast cancer cell lines)
Specification Breast cancer cell lines
Permeabilization Yes - Triton-X100
Fixative Paraformaldehyde
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提交于 Nov 11 2014

Application Immunohistochemistry (Frozen sections)
Blocking step (agent) for 1 hour(s) and 30 minute(s) · Concentration: 2% · Temperature: 25°C
Sample Human Tissue sections (Human Skin)
Specification Human Skin
Permeabilization Yes - 0.1% tritonx-100
Fixative Paraformaldehyde
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提交于 May 09 2014

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citric acid
Sample Mouse Tissue sections (Colon)
Specification Colon
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative Formaldehyde
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提交于 Feb 06 2014

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Antigen retrieval step Heat mediated
Sample Horse Tissue sections (Horse full thickness skin samples)
Specification Horse full thickness skin samples
Permeabilization No
Fixative Acetone
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Dr. Jan Spaas

Verified customer

提交于 Oct 17 2013

Thank you very much for your interest in our Anti-Cytokeratin 14 anitbodies. While ab53115 does not meet our criteria for possible use in Bos taurus accordign to the information made available to us we believe that ab7800 may be able to recognize this ...

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Thank you for your updates.

Could you please confirm the Abcam Order Number, it seems this information is missing from the previous correspondence.

As you know, in the event that a product is not functioning in the applications/spec...

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Thank you for the product information of the secondary antibodies.

Since this primary antibody (ab7800) has worked in ICC/IF studies and the secondary antibody was not the same secondary as used in Flow experiments, it is likely that the seco...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"