概述

  • 产品名称Anti-Cytokeratin 13抗体
    参阅全部 Cytokeratin 13 一抗
  • 描述
    兔多克隆抗体to Cytokeratin 13
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Sheep, Rabbit, Horse, Cow, Cat, Dog, Pig, Zebrafish
  • 免疫原

    A region within synthetic peptide: AQLSELRSEM ECQNQEYKML LDIKTRLEQE IATYRSLLEG QDAKKRQPP, corresponding to C terminal amino acids 372-420 of Human Cytokeratin 13

  • 阳性对照
    • WB: Jurkat cell lysate. IHC-P: Human lung tissue.

性能

应用

Our Abpromise guarantee covers the use of ab58744 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 2.5 µg/ml. Detects a band of approximately 41 kDa (predicted molecular weight: 50 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.

靶标

  • 组织特异性Expressed in some epidermal sweat gland ducts (at protein level) and in exocervix, esophagus and placenta.
  • 疾病相关Defects in KRT13 are a cause of white sponge nevus of cannon (WSN) [MIM:193900]. WSN is a rare autosomal dominant disorder which predominantly affects non-cornified stratified squamous epithelia. Clinically, it is characterized by the presence of soft, white, and spongy plaques in the oral mucosa. The characteristic histopathologic features are epithelial thickening, parakeratosis, and vacuolization of the suprabasal layer of oral epithelial keratinocytes. Less frequently the mucous membranes of the nose, esophagus, genitalia and rectum are involved.
  • 序列相似性Belongs to the intermediate filament family.
  • 翻译后修饰O-glycosylated; glycans consist of single N-acetylglucosamine residues.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 47 kDa cytokeratin antibody
    • CK-13 antibody
    • CK13 antibody
    • Cytokeratin 13 antibody
    • Cytokeratin-13 antibody
    • K13 antibody
    • K1C13_HUMAN antibody
    • Ka13 antibody
    • Keratin 13 antibody
    • Keratin antibody
    • keratin type I cytoskeletal 13 antibody
    • Keratin-13 antibody
    • Krt-1.13 antibody
    • Krt1-13 antibody
    • KRT13 antibody
    • MGC161462 antibody
    • MGC3781 antibody
    • type I cytoskeletal 13 antibody
    • Type I keratin Ka13 antibody
    • WSN2 antibody
    see all

Anti-Cytokeratin 13 antibody 图像

  • Anti-Cytokeratin 13 antibody (ab58744) at 2.5 µg/ml + Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti Rabbit IgG at 1/50,000

    Predicted band size : 50 kDa
    Observed band size : 41 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung tissue labelling Cytokeratin 13 with ab58744 at 4-8µg/ml. Arrows indicate positively labelled alveolar cells. Magnification: 400X.

Anti-Cytokeratin 13 antibody (ab58744)参考文献

This product has been referenced in:
  • Meng Q  et al. Eyelid closure in embryogenesis is required for ocular adnexa development. Invest Ophthalmol Vis Sci 55:7652-61 (2014). Read more (PubMed: 25377219) »
  • Fulzele A  et al. Proteomic profile of keratins in cancer of the gingivo buccal complex: Consolidating insights for clinical applications. J Proteomics 91C:242-258 (2013). IHC-P ; Human . Read more (PubMed: 23876858) »

See all 3 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (lung)
Specification lung
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate pH6.0
Permeabilization No
Blocking step Serum as blocking agent for 10 minute(s) · Concentration: 1.5%
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提交于 Mar 05 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"