Anti-Cytokeratin 10抗体[DE-K10] (ab9026)


  • 产品名称Anti-Cytokeratin 10抗体[DE-K10]
    参阅全部 Cytokeratin 10 一抗
  • 描述
    小鼠单克隆抗体[DE-K10] to Cytokeratin 10
  • 特异性Reacts with keratinizing stratified epithelia and in differentiated areas of highly differentiated squamous cell carcinomas.
  • 经测试应用适用于: IHC-Fr, ICC, IHC-P, WB, Flow Cytmore details
  • 种属反应性
    与反应: Mouse, Sheep, Cat, Dog, Human
  • 免疫原

    Cytoskeletal preparation extracted from human epidermis


  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.09% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • 纯度Protein G purified
  • 克隆单克隆
  • 克隆编号DE-K10
  • 骨髓瘤Sp2/0
  • 同种型IgG1
  • 轻链类型kappa
  • 研究领域


Our Abpromise guarantee covers the use of ab9026 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr Use at an assay dependent concentration.
ICC Use at an assay dependent concentration.
IHC-P 1/100.
WB 1/100 - 1/1000.
Flow Cyt 1/100 - 1/200. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.


  • 组织特异性Seen in all suprabasal cell layers including stratum corneum.
  • 疾病相关Defects in KRT10 are a cause of bullous congenital ichthyosiform erythroderma (BCIE) [MIM:113800]; also known as epidermolytic hyperkeratosis (EHK) or bullous erythroderma ichthyosiformis congenita of Brocq. BCIE is an autosomal dominant skin disorder characterized by widespread blistering and an ichthyotic erythroderma at birth that persist into adulthood. Histologically there is a diffuse epidermolytic degeneration in the lower spinous layer of the epidermis. Within a few weeks from birth, erythroderma and blister formation diminish and hyperkeratoses develop.
    Defects in KRT10 are a cause of ichthyosis annular epidermolytic (AEI) [MIM:607602]; also known as cyclic ichthyosis with epidermolytic hyperkeratosis. AEI is a skin disorder resembling bullous congenital ichthyosiform erythroderma. Affected individuals present with bullous ichthyosis in early childhood and hyperkeratotic lichenified plaques in the flexural areas and extensor surfaces at later ages. The feature that distinguishes AEI from BCIE is dramatic episodes of flares of annular polycyclic plaques with scale, which coalesce to involve most of the body surface and can persist for several weeks or even months.
  • 序列相似性Belongs to the intermediate filament family.
  • Information by UniProt
  • 数据库链接
  • 别名
    • BCIE antibody
    • BIE antibody
    • CK 10 antibody
    • CK-10 antibody
    • Cytokeratin-10 antibody
    • EHK antibody
    • K10 antibody
    • K1C10_HUMAN antibody
    • Keratin 10 antibody
    • Keratin 10 type I antibody
    • Keratin antibody
    • Keratin type i cytoskeletal 10 antibody
    • Keratin type I cytoskeletal 59 kDa antibody
    • Keratin-10 antibody
    • Keratin10 antibody
    • KPP antibody
    • KRT10 antibody
    • type I cytoskeletal 10 antibody
    see all

Anti-Cytokeratin 10 antibody [DE-K10] 图像

  • IHC image on a frozen section of  dog skin showing its strong reactivity in the keratinizing epidermal cells.

  • IHC image of ab9026 staining in Human cervix formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab9026, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Mesenchymal-epidermal interactions assessed by heterologous organotypic co-cultures (OTC). Heterologous OTC consisting of mice deficient for cathepsin L +/+ fibroblasts in collagen type I gels topped by normal human primary keratinocytes were grown air-exposed for 7 days. Paraffin sections were stained in haematoxylin and eosin (HE) or by immunohistochemistry for the proliferation marker Ki67 (Ki67, brown nuclear staining) and the differentiation markers cytokeratin 10 (K10, ab9026 1/100 dilution, brown staining) and transglutaminase (TG, brown staining). Bar 100 µm.

Anti-Cytokeratin 10 antibody [DE-K10] (ab9026)参考文献

This product has been referenced in:
  • Zhao H  et al. DNA Damage-Inducible Transcript 4 Is an Innate Surveillant of Hair Follicular Stress in Vitamin D Receptor Knockout Mice and a Regulator of Wound Re-Epithelialization. Int J Mol Sci 17:N/A (2016). IHC-P, ICC/IF ; Mouse . Read more (PubMed: 27898044) »
  • Pearson HB  et al. The polarity protein Scrib mediates epidermal development and exerts a tumor suppressive function during skin carcinogenesis. Mol Cancer 14:169 (2015). Read more (PubMed: 26376988) »

See all 13 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Horse Tissue sections (SKIN)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: TRIS-EDTA PH9
Permeabilization Yes - 0,05% TX 100
Specification SKIN
Blocking step Serum as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative Formaldehyde

Dr. Vincenzo Miragliotta

Verified customer

提交于 Jun 05 2017

Application Western blot
Sample Pig Tissue lysate - whole (skin)
Gel Running Conditions Reduced Denaturing
Loading amount 20 µg
Treatment nurn wound with stem cell treatment
Specification skin
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Abcam user community

Verified customer

提交于 May 20 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 0.05% typsin
Sample Human Tissue sections (skin)
Specification skin
Permeabilization No
Fixative Formaldehyde

Abcam user community

Verified customer

提交于 Feb 23 2015

I have just received the following information from the supplier of ab9026: "1) Yes please, you can offer your customer a free (replacement) batch of our other keratin 10 ab9025. 2)The question is however, whether this will be of any help. Ab902...

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I'm sorry to hear you are having problem with ab9026. Spotting can be frequently caused by the blocking agent you use, I would therefore try filtering it and leaving on the membrane for 30min, then rinsing the membrane with TBST. Incubate the prima...

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The exact epitope of Ab9026 is not known. I'm afraid you just have to try and see if your truncated protein is still recognized by Ab9026.