Anti-Cytokeratin 10 + 13抗体[DE-K13] (ab9383)

概述

  • 产品名称Anti-Cytokeratin 10 + 13抗体[DE-K13]
  • 描述
    小鼠单克隆抗体[DE-K13] to Cytokeratin 10 + 13
  • 特异性This antibody reacts with cytokeratin 10 and 13 in fixed cells and frozen tissue, but exclusively with cytokeratin 13 in formalin fixed paraffin embedded tissue.
  • 经测试应用适用于: Flow Cyt, ICC, IHC-Fr, WB, IHC-Pmore details
  • 种属反应性
    与反应: Cat, Dog, Human, Zebrafish
  • 免疫原

    Cytoskeletal preparation extracted from human ectocervical epithelium

性能

应用

Our Abpromise guarantee covers the use of ab9383 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt 1/100 - 1/200. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
ICC Use at an assay dependent concentration. Reacts with cytokeratin 10 and 13 in fixed cells.
IHC-Fr Use at an assay dependent concentration. Reacts with cytokeratin 10 and 13 in frozen tissues.
WB 1/100 - 1/1000.
IHC-P 1/100 - 1/200. Perform enzymatic antigen retrieval before commencing with IHC staining protocol. Reacts exclusively with cytokeratin 13 in formalin-fixed, paraffin-embedded tissues.

靶标

Anti-Cytokeratin 10 + 13 antibody [DE-K13] 图像

  • ab9383 staining Cytokeratin 10 + 13 in human urogenital tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
    Tissue was fixed in paraformaldehyde and a heat mediated antigen retrieval step was performed using sodium citrate buffer. Samples were then blocked using 5% serum for 1 hour then incubated with ab9383 at a 1/100 dilution for 2 hours at 37°C. The secondary used was a Cy3 conjugated donkey anti-mouse monoclonal used at a 1/500 dilution.

    See Abreview

  • Immunofluorescence staining of a 7 days old zebrafish embryo

Anti-Cytokeratin 10 + 13 antibody [DE-K13] (ab9383)参考文献

This product has been referenced in:
  • Ganor Y  et al. The adult penile urethra is a novel entry site for HIV-1 that preferentially targets resident urethral macrophages. Mucosal Immunol : (2012). Human . Read more (PubMed: 23187317) »

See 1 Publication for this product

Product Wall

Application Immunohistochemistry (Frozen sections)
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C
Sample Monkey Tissue sections (skin)
Specification skin
Permeabilization No
Fixative Acetone
Username

Abcam user community

Verified customer

提交于 Jul 09 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Human Urogenital)
Specification Human Urogenital
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Sodium Citrate
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Username

Abcam user community

Verified customer

提交于 Nov 01 2011

Merci pour votre Abreview. J'ai résolu le problème. Le numéro de lot n'était pas correct (un signe - de trop entre GR et le numéro). Votre Abreview est maintenant publiée et le code promotionnel est activé. Merci encore de votre collaboration.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"