Anti-Cytokeratin 1抗体(ab93652)

概述

  • 产品名称
    Anti-Cytokeratin 1抗体
    参阅全部 Cytokeratin 1 一抗
  • 描述
    兔多克隆抗体to Cytokeratin 1
  • 经测试应用
    适用于: ICC/IF, WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to Human Cytokeratin 1 (internal sequence).
    Database link: NM_006121

  • 阳性对照
    • WB: Fetal skin lysate IHC-P: Skin tissue

性能

应用

Our Abpromise guarantee covers the use of ab93652 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent concentration.
WB 1/200 - 1/1000. Predicted molecular weight: 66 kDa.
IHC-P 1/100 - 1/500.

靶标

  • 功能
    May regulate the activity of kinases such as PKC and SRC via binding to integrin beta-1 (ITB1) and the receptor of activated protein kinase C (RACK1/GNB2L1).
  • 组织特异性
    The source of this protein is neonatal foreskin. The 67-kDa type II keratins are expressed in terminally differentiating epidermis.
  • 疾病相关
    Defects in KRT1 are a cause of bullous congenital ichthyosiform erythroderma (BCIE) [MIM:113800]; also known as epidermolytic hyperkeratosis (EHK) or bullous erythroderma ichthyosiformis congenita of Brocq. BCIE is an autosomal dominant skin disorder characterized by widespread blistering and an ichthyotic erythroderma at birth that persist into adulthood. Histologically there is a diffuse epidermolytic degeneration in the lower spinous layer of the epidermis. Within a few weeks from birth, erythroderma and blister formation diminish and hyperkeratoses develop.
    Defects in KRT1 are the cause of ichthyosis hystrix Curth-Macklin type (IHCM) [MIM:146590]. IHCM is a genodermatosis with severe verrucous hyperkeratosis. Affected individuals manifest congenital verrucous black scale on the scalp, neck, and limbs with truncal erythema, palmoplantar keratoderma and keratoses on the lips, ears, nipples and buttocks.
    Defects in KRT1 are a cause of palmoplantar keratoderma non-epidermolytic (NEPPK) [MIM:600962]. NEPKK is a dermatological disorder characterized by focal palmoplantar keratoderma with oral, genital, and follicular lesions.
    Defects in KRT1 are a cause of ichthyosis annular epidermolytic (AEI) [MIM:607602]; also known as cyclic ichthyosis with epidermolytic hyperkeratosis. AEI is a skin disorder resembling bullous congenital ichthyosiform erythroderma. Affected individuals present with bullous ichthyosis in early childhood and hyperkeratotic lichenified plaques in the flexural areas and extensor surfaces at later ages. The feature that distinguishes AEI from BCIE is dramatic episodes of flares of annular polycyclic plaques with scale, which coalesce to involve most of the body surface and can persist for several weeks or even months.
    Defects in KRT1 are the cause of palmoplantar keratoderma striate type 3 (SPPK3) [MIM:607654]; also known as keratosis palmoplantaris striata III. SPPK3 is a dermatological disorder affecting palm and sole skin where stratum corneum and epidermal layers are thickened. There is no involvement of non-palmoplantar skin, and both hair and nails are normal.
  • 序列相似性
    Belongs to the intermediate filament family.
  • 翻译后修饰
    Undergoes deimination of some arginine residues (citrullination).
  • 细胞定位
    Cell membrane. Located on plasma membrane of neuroblastoma NMB7 cells.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 67 kDa cytokeratin antibody
    • CK-1 antibody
    • CK1 antibody
    • Cytokeratin-1 antibody
    • Cytokeratin1 antibody
    • EHK antibody
    • EHK1 antibody
    • Epidermolytic hyperkeratosis 1 antibody
    • EPPK antibody
    • Hair alpha protein antibody
    • K1 antibody
    • K2C1_HUMAN antibody
    • Keratin antibody
    • Keratin type II cytoskeletal 1 antibody
    • Keratin-1 antibody
    • Keratin1 antibody
    • KRT 1 antibody
    • Krt1 antibody
    • KRT1A antibody
    • NEPPK antibody
    • type II cytoskeletal 1 antibody
    • Type II keratin Kb1 antibody
    • Type-II keratin Kb1 antibody
    see all

图片

  • ab93652, at a 1/100 dilution, staining Cytokeratin 1 in the membrane of formalin fixed, paraffin embedded skin tissue section by Immunohistochemistry.
  • Anti-Cytokeratin 1 antibody (ab93652) at 1/500 dilution + fetal skin lysate

    Predicted band size : 66 kDa
  • ab14404 staining Cytokeratin 1 in Human LNCaP, prostate cancer cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde and blocked with 1% Donkey serum for 1 hour at 22°C. Samples were incubated with primary antibody (1/100 in 1% Donkey serum in PBST) for 1 hour at 22°C. A DyLight® 594-conjugated Donkey anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody. Red - cytokeratin 1, Green - Golgi marker ST3Gal1, Blue - nucleus (DAPI). 

    See Abreview

文献

This product has been referenced in:
  • Cao L  et al. Keratin mediates the recognition of apoptotic and necrotic cells through dendritic cell receptor DEC205/CD205. Proc Natl Acad Sci U S A 113:13438-13443 (2016). Read more (PubMed: 27821726) »
  • Hill DS  et al. A Novel Fully Humanized 3D Skin Equivalent to Model Early Melanoma Invasion. Mol Cancer Ther 14:2665-73 (2015). IHC-P ; Human . Read more (PubMed: 26330548) »

See all 3 Publications for this product

客户评价及客户问答

I am sorry that this antibody did not perform as stated on the datasheet. If payment has already been made on the original order and you wish to receive a refund, please ask your purchasing department to contact our accounting department so that we may...

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Application
Western blot
Loading amount
35 µg
Gel Running Conditions
Reduced Denaturing (12% SDS-PAGE)
Sample
Rat Cell lysate - other (Rat hepatocytes plasma membrane fraction)
Specification
Rat hepatocytes plasma membrane fraction
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Dr. Armen Petrosyan

Verified customer

提交于 Oct 28 2014

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Sample
Human Cell (LNCaP, prostate cancer cells)
Specification
LNCaP, prostate cancer cells
Permeabilization
No
Fixative
Formaldehyde
Username

Dr. Armen Petrosyan

Verified customer

提交于 Oct 17 2013

Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing (4-15% gradient polyacrilamide gel)
Sample
Human Cell lysate - whole cell (LNCaP, prostate cancer cells)
Specification
LNCaP, prostate cancer cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Dr. Armen Petrosyan

Verified customer

提交于 Oct 17 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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