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Our Abpromise guarantee covers the use of ab93652 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration.|
|WB||1/200 - 1/1000. Predicted molecular weight: 66 kDa.|
|IHC-P||1/100 - 1/500.|
ab14404 staining Cytokeratin 1 in Human LNCaP, prostate cancer cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde and blocked with 1% Donkey serum for 1 hour at 22°C. Samples were incubated with primary antibody (1/100 in 1% Donkey serum in PBST) for 1 hour at 22°C. A DyLight® 594-conjugated Donkey anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody. Red - cytokeratin 1, Green - Golgi marker ST3Gal1, Blue - nucleus (DAPI).
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