Anti-Cytokeratin 1 - 8抗体[AE3], prediluted (ab34255)

概述

  • 产品名称Anti-Cytokeratin 1 - 8抗体[AE3], prediluted
    参阅全部 Cytokeratin 1 - 8 一抗
  • 描述
    小鼠单克隆抗体[AE3] to Cytokeratin 1 - 8, prediluted
  • 特异性ab32455 reacts with most of the Basic (Type II) keratins. Including epidermal cell keratin of 86 and 58kD and skin at 58kD, 65kD and 67kD. Broad keratin reactivity is found on human epidermal cell, oesophagus and cornea. Since each epithelium contains at least one basic keratin, AE3 is broadly reactive and stains positively almost all epithelia and their neoplasms.
  • 经测试应用适用于: IHC-FoFr, IHC-P, IHC-Frmore details
  • 种属反应性
    与反应: Mouse, Rat, Rabbit, Chicken, Cow, Fish, Monkey, Macaque Monkey
  • 免疫原

    Tissue/ cell preparation - Human epidermal cells.

  • 阳性对照
    • Human epidermal cells, oesophagus, cornea.

性能

应用

Our Abpromise guarantee covers the use of ab34255 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-FoFr
IHC-P
IHC-Fr
  • 应用说明IHC-Fr: Use neat
    IHC-FoFr: Use neat.
    IHC-P: Use neat. This product requires protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    Anti-Cytokeratin 1 - 8 antibody [AE3], prediluted (ab34255)参考文献

    This product has been referenced in:
    • Tsubura A  et al. Immunophenotypic difference of keratin expression in normal mammary glandular cells from five different species. Acta Anat (Basel) 140:287-93 (1991). Read more (PubMed: 1714223) »
    • Eichner R  et al. Classification of epidermal keratins according to their immunoreactivity, isoelectric point, and mode of expression. J Cell Biol 98:1388-96 (1984). Read more (PubMed: 6201491) »

    See all 4 Publications for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Frozen sections)
    Sample Chicken Tissue sections (chicken embryo epicardium)
    Specification chicken embryo epicardium
    Fixative Paraformaldehyde
    Permeabilization Yes - 0,2% Triton X-100 in PBS, 10'
    Blocking step Serum as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 37°C
    Username

    Mrs. Elena Astanina

    Verified customer

    提交于 Feb 20 2008

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"