Anti-Cytohesin 2抗体[10A12] (ab2728)

概述

  • 产品名称Anti-Cytohesin 2抗体[10A12]
    参阅全部 Cytohesin 2 一抗
  • 描述
    小鼠单克隆抗体[10A12] to Cytohesin 2
  • 特异性Detects recombinant human cytohesin-2 (ARNO).
  • 经测试应用适用于: ICC/IF, IHC-P, IPmore details
    不适用于: WB
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Recombinant full length protein (His-tag) corresponding to Cytohesin 2.

性能

应用

Our Abpromise guarantee covers the use of ab2728 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/20.
IHC-P 1/25 - 1/200.
IP Use at an assay dependent concentration.
  • 应用说明Is unsuitable for WB.
  • 靶标

    • 功能Acts as a guanine-nucleotide exchange factor (GEF). Promotes guanine-nucleotide exchange on ARF1, ARF3 and ARF6. Promotes the activation of ARF factors through replacement of GDP with GTP. The cell membrane form, in association with ARL4 proteins, recruits ARF6 to the plasma membrane.
    • 组织特异性Ubiquitous.
    • 序列相似性Contains 1 PH domain.
      Contains 1 SEC7 domain.
    • 结构域The PH domain is necessary and sufficient for plasma membrane relocalization.
    • 细胞定位Cell membrane. Cytoplasm. Both isoform 1 and isoform 2 are recruited to the cell membrane through its association with ARL4A, ARL4C and ARL4D. Requires also interaction with phosphoinositides for targeting to plasma membrane.
    • Information by UniProt
    • 数据库链接
    • 别名
      • ARF exchange factor antibody
      • ARF nucleotide binding site opener antibody
      • ARF nucleotide-binding site opener antibody
      • Arno antibody
      • ARNO protein antibody
      • CLM2 antibody
      • CTS18 antibody
      • CTS18.1 antibody
      • CYH2_HUMAN antibody
      • Cyth2 antibody
      • Cytohesin 2 antibody
      • Cytohesin-2 antibody
      • MGC137537 antibody
      • MGC80440 antibody
      • PH antibody
      • PH, SEC7 and coiled-coil domain-containing protein 2 antibody
      • Pleckstrin homology Sec7 and coiled coil domains 2 antibody
      • Pleckstrin homology Sec7 and coiled coil domains protein 2 antibody
      • Protein ARNO antibody
      • PSCD2 antibody
      • PSCD2L antibody
      • PSCD2L, formerly antibody
      • SEC7 and coiled-coil domain-containing protein 2 antibody
      • Sec7 antibody
      • SEC7 homolog B antibody
      • Sec7B antibody
      • SEC7L antibody
      • Sec7p L antibody
      • Sec7p-like antibody
      • Sec7pL antibody
      see all

    Anti-Cytohesin 2 antibody [10A12] 图像

    • Immunocytochemistry/Immunofluorescence analysis of Cytohesin 2 in HeLa cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with ab2728 (1:100) overnight at 4°C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Cytohesin 2 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.

    • Immunocytochemistry/Immunofluorescence analysis of Cytohesin 2 in HepG2 cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with ab2728 (1:100) overnight at 4°C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Cytohesin 2 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.

    • Immunocytochemistry/Immunofluorescence analysis of Cytohesin 2 in NIH-3T3 cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with ab2728 (1:100) overnight at 4°C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Cytohesin 2 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) was performed on normal biopsies of deparaffinized human colon carcinoma tissue. Heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature and incubated with ab2728 (1:50) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) was performed on normal biopsies of deparaffinized human stomach tissue. Heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature and incubated with ab2728 (1:50) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

    Anti-Cytohesin 2 antibody [10A12] (ab2728)参考文献

    ab2728 has not yet been referenced specifically in any publications.

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