ab57713 at 3µg/ml, staining cytoglobin in human heart tissue by IHC-P.
Western blot - Cytoglobin antibody (ab57713)
All lanes : Anti-Cytoglobin antibody (ab57713) at 1/500 dilution
Lane 1 : Transfected 293T lysate at 25 µg Lane 2 : Non-transfected 293T lysate at 25 µg
Predicted band size : 21 kDa
Flow Cytometry-Anti-Cytoglobin antibody(ab57713)
Overlay histogram showing HeLa cells stained with ab57713 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab57713, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.