概述

  • 产品名称
    Anti-Cytoglobin抗体
    参阅全部 Cytoglobin 一抗
  • 描述
    小鼠单克隆抗体to Cytoglobin
  • 经测试应用
    适用于: WB, ELISA, IHC-P, Flow Cytmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant full length protein, corresponding to amino acids 1-191 of Human Cytoglobin

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液
    Preservative: None
    PBS, pH 7.2
  • Concentration information loading...
  • 纯度
    Protein G purified
  • 克隆
    单克隆
  • 同种型
    IgG1
  • 轻链类型
    kappa
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab57713 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 21 kDa.
ELISA Use at an assay dependent concentration.
IHC-P Use a concentration of 3 µg/ml.
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

靶标

  • 功能
    May have a protective function during conditions of oxidative stress. May be involved in intracellular oxygen storage or transfer.
  • 组织特异性
    Ubiquitously expressed. Highest expression in heart, stomach, bladder and small intestine.
  • 序列相似性
    Belongs to the globin family.
  • 细胞定位
    Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Cygb antibody
    • CYGB_HUMAN antibody
    • Cytoglobin antibody
    • Hgb antibody
    • Histoglobin antibody
    • STAP antibody
    • Stellate cell activation associated protein antibody
    • Stellate cell activation-associated protein antibody
    see all

Anti-Cytoglobin antibody 图像



  • Predicted band size : 21 kDa
  • ab57713 at 3µg/ml, staining cytoglobin in human heart tissue by IHC-P.
  • All lanes : Anti-Cytoglobin antibody (ab57713) at 1/500 dilution

    Lane 1 : Transfected 293T lysate at 25 µg
    Lane 2 : Non-transfected 293T lysate at 25 µg


    Predicted band size : 21 kDa
  • Overlay histogram showing HeLa cells stained with ab57713 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab57713, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

Anti-Cytoglobin antibody (ab57713)参考文献

This product has been referenced in:
  • Xu ZJ  et al. Liuwei Dihuang pill treats diabetic nephropathy in rats by inhibiting of TGF-ß/SMADS, MAPK, and NF-kB and upregulating expression of cytoglobin in renal tissues. Medicine (Baltimore) 96:e5879 (2017). Read more (PubMed: 28099346) »
  • Wu X  et al. CUG-binding protein 1 regulates HSC activation and liver fibrogenesis. Nat Commun 7:13498 (2016). IF ; Human . Read more (PubMed: 27853137) »

See all 3 Publications for this product

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