Anti-Cytochrome P450 Reductase抗体(ab89622)

概述

  • 产品名称Anti-Cytochrome P450 Reductase抗体
    参阅全部 Cytochrome P450 Reductase 一抗
  • 描述
    小鼠多克隆抗体to Cytochrome P450 Reductase
  • 经测试应用适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Full length Human protein (AAH34277.1)

  • 阳性对照
    • WB: liver lysate. ICC/IF: HeLa cells.

性能

应用

Our Abpromise guarantee covers the use of ab89622 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 77 kDa.
ICC/IF Use a concentration of 10 µg/ml.

靶标

  • 功能This enzyme is required for electron transfer from NADP to cytochrome P450 in microsomes. It can also provide electron transfer to heme oxygenase and cytochrome B5.
  • 疾病相关Defects in POR are the cause of adrenal hyperplasia variant type (AHV) [MIM:201750]; also known as Antley-Bixler syndrome-like phenotype with disordered steroidogenesis. AHV is a rare variant of congenital adrenal hyperplasia. It is an autosomal recessive disorder with apparent combined P450C17 and P450C21 deficiency. Affected girls are born with ambiguous genitalia, but their circulating androgens are low and virilization does not progress. Conversely, affected boys are sometimes born undermasculinized. Boys and girls can also present with bone malformations, in some cases resembling the pattern seen in patients with Antley-Bixler syndrome.
    Defects in POR are a cause of isolated disordered steroidogenesis (IDS) [MIM:201750].
  • 序列相似性In the C-terminal section; belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.
    Contains 1 FAD-binding FR-type domain.
    Contains 1 flavodoxin-like domain.
  • 细胞定位Endoplasmic reticulum membrane. Anchored to the ER membrane by its N-terminal hydrophobic region.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CPR antibody
    • CYPOR antibody
    • DKFZp686G04235 antibody
    • FLJ26468 antibody
    • NADPH Cytochrome P450 Reductase antibody
    • NADPH dependent cytochrome P450 reductase antibody
    • NADPH--cytochrome P450 reductase antibody
    • NCPR_HUMAN antibody
    • P450 (cytochrome) oxidoreductase antibody
    • P450 Cytochrome Oxidoreductase antibody
    • P450R antibody
    • por antibody
    see all

Anti-Cytochrome P450 Reductase antibody 图像

  • Anti-Cytochrome P450 Reductase antibody (ab89622) at 1 µg/ml + Human liver lysate at 50 µg

    Predicted band size : 77 kDa
  • All lanes : Anti-Cytochrome P450 Reductase antibody (ab89622) at 1 µg/ml

    Lane 1 : Cytochrome P450 Reductase transfected 293T cell lysate
    Lane 2 : Untransfected lysate

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 77 kDa
  • Immunofluorescence analysis of Cytochrome P450 Reductase expression in PFA-fixed HeLa cells using ab89622 at 10 µg/ml.

Anti-Cytochrome P450 Reductase antibody (ab89622)参考文献

ab89622 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (HEK283T)
Loading amount 200000 cells
Specification HEK283T
Gel Running Conditions Non-reduced Denaturing (10%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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提交于 Jun 20 2012

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I am very pleased to hear you would like to accept our offer and test ab89622 in mouse tissue. You are correct that you have to purchase the antibody ab89622 first, and then after submit...

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Thank you very much for your interest in ab89622.
We do not provide sample sizes of our antibodies but as we have not tested ab89622 in mouse tissue (although there is a 92% level of homology between human an mouse, so there is a very good chance ...

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