Anti-Cytochrome P450 Reductase抗体(ab39995)

概述

  • 产品名称Anti-Cytochrome P450 Reductase抗体
    参阅全部 Cytochrome P450 Reductase 一抗
  • 描述
    兔多克隆抗体to Cytochrome P450 Reductase
  • 经测试应用适用于: ICC/IF, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
    预测可用于: Rabbit, Chinese Hamster
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 250 - 350 of Human Cytochrome P450 Reductase.

    (Peptide available as ab41337.)

  • 阳性对照
    • This antibody gave a positive signal in mouse and rat liver tissue lysates and in the following whole cell lysates: PC12; HeLa; A431; HEK293; HepG2.

性能

应用

Our Abpromise guarantee covers the use of ab39995 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 1 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 77 kDa (predicted molecular weight: 77 kDa).

靶标

  • 功能This enzyme is required for electron transfer from NADP to cytochrome P450 in microsomes. It can also provide electron transfer to heme oxygenase and cytochrome B5.
  • 疾病相关Defects in POR are the cause of adrenal hyperplasia variant type (AHV) [MIM:201750]; also known as Antley-Bixler syndrome-like phenotype with disordered steroidogenesis. AHV is a rare variant of congenital adrenal hyperplasia. It is an autosomal recessive disorder with apparent combined P450C17 and P450C21 deficiency. Affected girls are born with ambiguous genitalia, but their circulating androgens are low and virilization does not progress. Conversely, affected boys are sometimes born undermasculinized. Boys and girls can also present with bone malformations, in some cases resembling the pattern seen in patients with Antley-Bixler syndrome.
    Defects in POR are a cause of isolated disordered steroidogenesis (IDS) [MIM:201750].
  • 序列相似性In the C-terminal section; belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.
    Contains 1 FAD-binding FR-type domain.
    Contains 1 flavodoxin-like domain.
  • 细胞定位Endoplasmic reticulum membrane. Anchored to the ER membrane by its N-terminal hydrophobic region.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CPR antibody
    • CYPOR antibody
    • DKFZp686G04235 antibody
    • FLJ26468 antibody
    • NADPH Cytochrome P450 Reductase antibody
    • NADPH dependent cytochrome P450 reductase antibody
    • NADPH--cytochrome P450 reductase antibody
    • NCPR_HUMAN antibody
    • P450 (cytochrome) oxidoreductase antibody
    • P450 Cytochrome Oxidoreductase antibody
    • P450R antibody
    • por antibody
    see all

Anti-Cytochrome P450 Reductase antibody 图像

  • All lanes : Anti-Cytochrome P450 Reductase antibody (ab39995) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : A431 whole cell lysate (ab7909)
    Lane 3 : HEK293 whole cell lysate (ab7902)
    Lane 4 : HepG2 whole cell lysate (ab7900)

    Lysates/proteins at 10 µg per lane.

    Secondary
    IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 77 kDa
    Observed band size : 77 kDa
    Additional bands at : 105 kDa,150 kDa. We are unsure as to the identity of these extra bands.
  • ICC/IF image of ab39995 stained human HeLa cells. The cells were methanol fixed (5 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab39995, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).

  • All lanes : Anti-Cytochrome P450 Reductase antibody (ab39995) at 1 µg/ml

    Lane 1 : Liver (Mouse) Tissue Lysate
    Lane 2 : Liver (Rat) Tissue Lysate
    Lane 3 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 77 kDa
    Observed band size : 77 kDa
    Additional bands at : 26 kDa,48 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 30 seconds

Anti-Cytochrome P450 Reductase antibody (ab39995)参考文献

ab39995 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Pig Cell lysate - whole cell (Pig Mammary Epithelial Cells)
Gel Running Conditions Reduced Denaturing (4-12% Bis-Tris)
Loading amount 10 µg
Specification Pig Mammary Epithelial Cells
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
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提交于 Feb 12 2016

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Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cell (rat intestinal epithelial cells (RIE-1))
Permeabilization Yes - 0.2% TritonX-100
Specification rat intestinal epithelial cells (RIE-1)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1.5% · Temperature: 25°C
Fixative Paraformaldehyde
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提交于 Feb 11 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Chinese Hamster Cell (Chinese hamster ovary cell)
Permeabilization Yes - 0.2% TritonX-100
Specification Chinese hamster ovary cell
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1.5% · Temperature: 25°C
Fixative Paraformaldehyde
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提交于 Feb 11 2016

Application Western blot
Sample Rat Cell lysate - whole cell (rat intestinal epithelial cells (RIE-1))
Gel Running Conditions Reduced Denaturing (4-12% Bis-Tris)
Loading amount 100000 cells
Treatment 100 nM Bortezomib for 16 hours
Specification rat intestinal epithelial cells (RIE-1)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
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提交于 Feb 09 2016

Application Western blot
Sample Chinese Hamster Cell lysate - whole cell (Chinese hamster ovary cell)
Gel Running Conditions Reduced Denaturing (4-12% Bis-Tris)
Loading amount 100000 cells
Treatment 100 nM Bortezomib for 16 hours
Specification Chinese hamster ovary cell
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
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提交于 Feb 09 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Spodoptera frugiperda (SF9 cells) Cell lysate - whole cell (sf9 cells)
Gel Running Conditions Reduced Denaturing (4-12% Bis-Tris)
Loading amount 100000 cells
Treatment 30 nM Bortezomib for 16 hours
Specification sf9 cells
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
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提交于 Feb 09 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application Flow Cytometry
Sample Mouse Cell (Mouse Lewis Lung Carcinoma cells)
Permeabilization Yes - 0.2% Triton X-100
Gating Strategy No gating
Specification Mouse Lewis Lung Carcinoma cells
Preparation Cell harvesting/tissue preparation method: trypsin
Sample buffer: Hanks’ Balanced Salt solution (HBSS)
Fixation Paraformaldehyde
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提交于 Jan 27 2016

Application IHC - Wholemount
Sample Human Tissue (Breast invasive ductal carcinoma)
Specification Breast invasive ductal carcinoma
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提交于 Dec 22 2015

Application IHC - Wholemount
Sample Mouse Tissue (Transplanted lung tumor section)
Specification Transplanted lung tumor section
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提交于 Dec 22 2015

Application Western blot
Sample African Green Monkey Cell lysate - whole cell (COS-7 cells)
Gel Running Conditions Reduced Denaturing (4-12% Bis-Tris)
Loading amount 100000 cells
Treatment 30 nM Bortezomib for 24 hours
Specification COS-7 cells
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
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提交于 Jun 27 2015

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