Anti-Cytochrome P450 3A4抗体(ab94334)

概述

  • 产品名称Anti-Cytochrome P450 3A4抗体
    参阅全部 Cytochrome P450 3A4 一抗
  • 描述
    兔多克隆抗体to Cytochrome P450 3A4
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Chimpanzee, Cynomolgus Monkey, Macaque Monkey, Marmoset (common), Orangutan
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 400 - 500 of Human Cytochrome P450 3A4 .

  • 阳性对照
    • This antibody gave a positive signal in Human Liver Tissue Lysate.

性能

应用

Our Abpromise guarantee covers the use of ab94334 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 58 kDa).

靶标

  • 功能Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It performs a variety of oxidation reactions (e.g. caffeine 8-oxidation, omeprazole sulphoxidation, midazolam 1'-hydroxylation and midazolam 4-hydroxylation) of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. The enzyme also hydroxylates etoposide.
  • 组织特异性Expressed in prostate and liver. According to some authors, it is not expressed in brain (PubMed:19094056). According to others, weak levels of expression are measured in some brain locations (PubMed:19359404 and PubMed:18545703). Also expressed in epithelium of the small intestine and large intestine, bile duct, nasal mucosa, kidney, adrenal cortex, epithelium of the gastric mucosa with intestinal metaplasia, gallbladder, intercalated ducts of the pancreas, chief cells of the parathyroid and the corpus luteum of the ovary (at protein level).
  • 序列相似性Belongs to the cytochrome P450 family.
  • 翻译后修饰Polyubiquitinated in the presence of AMFR and UBE2G1 and also STUB1/CHIP and UBE2D1 (in vitro).
  • 细胞定位Endoplasmic reticulum membrane. Microsome membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 1,8-cineole 2-exo-monooxygenase antibody
    • Albendazole monooxygenase antibody
    • Albendazole sulfoxidase antibody
    • CP33 antibody
    • CP34 antibody
    • CP3A4_HUMAN antibody
    • CYP3 antibody
    • CYP3A antibody
    • CYP3A3 antibody
    • CYP3A4 antibody
    • CYPIIIA3 antibody
    • CYPIIIA4 antibody
    • Cytochrome P450 3A3 antibody
    • Cytochrome P450 3A4 antibody
    • Cytochrome P450 family 3 subfamily A polypeptide 4 antibody
    • Cytochrome P450 HLp antibody
    • Cytochrome P450 NF-25 antibody
    • Cytochrome P450 subfamily IIIA polypeptide 4 antibody
    • cytochrome P450, subfamily IIIA (niphedipine oxidase), polypeptide 3 antibody
    • cytochrome P450, subfamily IIIA (niphedipine oxidase), polypeptide 4 antibody
    • Cytochrome P450-PCN1 antibody
    • Glucocorticoid inducible P450 antibody
    • HLP antibody
    • MGC126680 antibody
    • NF 25 antibody
    • NF25 antibody
    • Nifedipine oxidase antibody
    • P450 III steroid inducible antibody
    • P450 PCN1 antibody
    • P450, family III antibody
    • P450C3 antibody
    • P450PCN1 antibody
    • Quinine 3 monooxygenase antibody
    • Quinine 3-monooxygenase antibody
    • Taurochenodeoxycholate 6 alpha hydroxylase antibody
    • Taurochenodeoxycholate 6-alpha-hydroxylase antibody
    see all

Anti-Cytochrome P450 3A4 antibody 图像

  • Anti-Cytochrome P450 3A4 antibody (ab94334) at 1 µg/ml + Human liver tissue lysate - total protein (ab29889) at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 58 kDa
    Observed band size : 55 kDa (why is the actual band size different from the predicted?)


    Exposure time : 20 minutes
  • Anti-Cytochrome P450 3A4 antibody (ab94334) at 1 µg/ml + Recombinant Human Cytochrome P450 3A4 protein (ab114327) at 0.1 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 20 seconds

Anti-Cytochrome P450 3A4 antibody (ab94334)参考文献

ab94334 has not yet been referenced specifically in any publications.

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