Anti-Cytochrome P450 3A1抗体(ab22733)

概述

  • 产品名称Anti-Cytochrome P450 3A1抗体
    参阅全部 Cytochrome P450 3A1 一抗
  • 描述
    兔多克隆抗体to Cytochrome P450 3A1
  • 经测试应用适用于: WB, IHC-FoFr, IHC-Frmore details
  • 种属反应性
    与反应: Rat
  • 免疫原

    Synthetic peptide conjugated to KLH, taken from the C-terminus of rat Cytochrome P450 3A1.

  • 阳性对照
    • PCN stimulated rat liver and microsomal preparations of liver.

性能

应用

Our Abpromise guarantee covers the use of ab22733 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
IHC-FoFr
IHC-Fr
  • 应用说明IHC-F: Use at a dilution of up to 1/2000.
    IHC-Fr: Use at a dilution of up to 1/2000.
    WB: Use at an assay dependent dilution. Detects a band of approximately 58 kDa.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 相关性In animals, P-450 enzymes serve two major functions: (1) biosynthesis of steroids, fatty acids, and bile acids; (2) metabolism of endogenous and a wide variety of exogenous substrates, such as toxins and drugs. The four major families involved in drug metabolism are CYP 1,2,3, and 4. Cytochrome P450 3A4 [human-3A1 Rat] is abundantly expressed in liver and small intestine and is inducible by barbiturates, glucocorticoids and rifampicin.
    • 细胞定位Endoplasmic reticulum, membrane bound.
    • 数据库链接
    • 别名
      • CYP3A1 antibody
      • cytochrome P450, family 3, subfamily a, polypeptide 1 antibody

    Anti-Cytochrome P450 3A1 antibody 图像

    • ab22733 staining Cytochrome P450 3A1 in rat liver tissue sections by Immunohistochemistry (frozen sections). Tissue was fixed with acetone. Samples were then blocked with 2% serum for 1 hour at 20°C followed by incubation with the primary antibody, at a 1/500 dilution, for 1 hour at 20°C. An Atto 550 dye-conjugated goat anti-rabbit IgG polyclonal was used as secondary antibody at a 1/500.

      See Abreview

    Anti-Cytochrome P450 3A1 antibody (ab22733)参考文献

    This product has been referenced in:
    • Dublineau I  et al. Unexpected lack of deleterious effects of uranium on physiological systems following a chronic oral intake in adult rat. Biomed Res Int 2014:181989 (2014). WB ; Rat . Read more (PubMed: 24693537) »
    • Hassan HE  et al. Induction of xenobiotic receptors, transporters, and drug metabolizing enzymes by oxycodone. Drug Metab Dispos 41:1060-9 (2013). Rat . Read more (PubMed: 23439660) »

    See all 3 Publications for this product

    Product Wall

    Thank you for contacting us and I am sorry for the delay in getting back to you.

    I am pleased to hear that the replacement antibody provided has been working well for you. As I mentioned in my previous email, the homology of the CYP3A1 and CYP...

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    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Frozen sections)
    Sample Rat Tissue sections (Liver)
    Specification Liver
    Fixative Acetone 10' at 4°C
    Permeabilization No
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 20°C
    Username

    Abcam user community

    Verified customer

    提交于 Oct 12 2010

    I can confirm that the antibodies were raised to a synthetic hexapeptide which includes the C-terminal of rat CYP3A110: H-Cys-Ile-Ile-Thr-Gly-Ser-OH. Both antibodies work equally well in western blotting and we cannot recommend one over the other, w...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"