Anti-Cytochrome P450 2E1抗体(ab53945)

概述

  • 产品名称Anti-Cytochrome P450 2E1抗体
    参阅全部 Cytochrome P450 2E1 一抗
  • 描述
    兔多克隆抗体to Cytochrome P450 2E1
  • 特异性ab53945 recognizes Cytochrome P450 2E1.
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Rat, Human
  • 免疫原

    Synthetic peptide (Human) Cytochrome P450 2E1

  • 阳性对照
    • Pyridine-treated kidney microsome.

应用

Our Abpromise guarantee covers the use of ab53945 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa).
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能Metabolizes several precarcinogens, drugs, and solvents to reactive metabolites. Inactivates a number of drugs and xenobiotics and also bioactivates many xenobiotic substrates to their hepatotoxic or carcinogenic forms.
  • 序列相似性Belongs to the cytochrome P450 family.
  • 细胞定位Endoplasmic reticulum membrane. Microsome membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 4-nitrophenol 2-hydroxylase antibody
    • CP2E1_HUMAN antibody
    • CPE1 antibody
    • CYP2E antibody
    • Cyp2e1 antibody
    • CYPIIE1 antibody
    • Cytochrome P450 2E1 antibody
    • Cytochrome P450 isozyme 3A antibody
    • Cytochrome P450 LM3A antibody
    • Cytochrome P450, family 2, subfamily e, polypeptide 1 antibody
    • cytochrome P450, subfamily IIE (ethanol-inducible), polypeptide 1 antibody
    • Cytochrome P450, subfamily IIE antibody
    • Cytochrome P450-ALC antibody
    • Cytochrome P450-J antibody
    • Cytochrome P450RLM6 antibody
    • EC 1.14.13.- antibody
    • EC 1.14.13.n7 antibody
    • Ethanol-inducible P450 antibody
    • Flavoprotein-linked monooxygenase antibody
    • MGC133529 antibody
    • Microsomal monooxygenase antibody
    • OTTHUMP00000020813 antibody
    • OTTHUMP00000046571 antibody
    • P450 ALC antibody
    • P450-J antibody
    • P450C2E antibody
    • P450J antibody
    • P450RLM6 antibody
    • Xenobiotic monooxygenase antibody
    see all

Anti-Cytochrome P450 2E1 antibody 图像

  • All lanes : Anti-Cytochrome P450 2E1 antibody (ab53945) at 1/1000 dilution

    Lane 1 : Control kidney microsome
    Lane 2 : Pyridine-treated kidney microsome

    Lysates/proteins at 10 µg per lane.


    Predicted band size : 57 kDa
    Observed band size : 57 kDa
  • IHC image of ab53945 staining in human liver carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab53945, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-Cytochrome P450 2E1 antibody (ab53945)参考文献

ab53945 has not yet been referenced specifically in any publications.

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