Anti-Cytochrome P450 2E1抗体(ab28146)

概述

  • 产品名称Anti-Cytochrome P450 2E1抗体
    参阅全部 Cytochrome P450 2E1 一抗
  • 描述
    兔多克隆抗体to Cytochrome P450 2E1
  • 特异性The antibody detects a ~50-55 kDa protein, corresponding to the apparent molecular mass of cytochrome P450 IIE1 on SDS-PAGE immunoblots.
  • 经测试应用适用于: IHC-Fr, WB, ICC/IF, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat, Rabbit, Horse, Guinea pig, Dog, Human, Monkey
  • 免疫原

    Full length native protein (purified) (Rat)

  • 常规说明


    The antibody has been shown to inhibit microsomal benzene metabolism

性能

应用

Our Abpromise guarantee covers the use of ab28146 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr 1/300. PubMed: 17021035
WB 1/5000. Predicted molecular weight: 50-55 kDa. This dilution was sufficient for detection of Cytochrome P450 in 20ug of human liver microsomes by colorimetric immunoblot analysis using Goat anti-Rabbit IgG:AP as the secondary antibody.
ICC/IF 1/1000.
IHC-P Use at an assay dependent concentration. PubMed: 24155903

靶标

  • 功能Metabolizes several precarcinogens, drugs, and solvents to reactive metabolites. Inactivates a number of drugs and xenobiotics and also bioactivates many xenobiotic substrates to their hepatotoxic or carcinogenic forms.
  • 序列相似性Belongs to the cytochrome P450 family.
  • 细胞定位Endoplasmic reticulum membrane. Microsome membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 4-nitrophenol 2-hydroxylase antibody
    • CP2E1_HUMAN antibody
    • CPE1 antibody
    • CYP2E antibody
    • Cyp2e1 antibody
    • CYPIIE1 antibody
    • Cytochrome P450 2E1 antibody
    • Cytochrome P450 isozyme 3A antibody
    • Cytochrome P450 LM3A antibody
    • Cytochrome P450, family 2, subfamily e, polypeptide 1 antibody
    • cytochrome P450, subfamily IIE (ethanol-inducible), polypeptide 1 antibody
    • Cytochrome P450, subfamily IIE antibody
    • Cytochrome P450-ALC antibody
    • Cytochrome P450-J antibody
    • Cytochrome P450RLM6 antibody
    • EC 1.14.13.- antibody
    • EC 1.14.13.n7 antibody
    • Ethanol-inducible P450 antibody
    • Flavoprotein-linked monooxygenase antibody
    • MGC133529 antibody
    • Microsomal monooxygenase antibody
    • OTTHUMP00000020813 antibody
    • OTTHUMP00000046571 antibody
    • P450 ALC antibody
    • P450-J antibody
    • P450C2E antibody
    • P450J antibody
    • P450RLM6 antibody
    • Xenobiotic monooxygenase antibody
    see all

Anti-Cytochrome P450 2E1 antibody 图像

  • ab28146 staining cells of rat liver sections by IHC-Fr.  Cells were fixed in acetone and blocked with 1% BSA for 5 minutes at 25°C prior to incubating with ab28146 diluted 1/300 for 30 minutes at 25°C.  An Alexa Fluor® 594 conjugated goat anti-rabbit antibody was used as the secondary.

    Double IF labeling with CYP2E1 (Red) and dipeptidyl peptidase IV (Green)

    See Abreview

  • ICC/IF image of ab28146 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28146, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • All lanes : Anti-Cytochrome P450 2E1 antibody (ab28146) at 1/1000 dilution

    Lane 1 : Molecular weight marker
    Lane 2 : Cell lysates prepared from human liver microsomes
    Lane 3 : Cell lysates prepared from rat liver microsomes
    Lane 4 : Cell lysates prepared from mouse liver microsomes
    Lane 5 : Cell lysates prepared from rabbit liver microsomes


    Predicted band size : 50-55 kDa
  • All lanes : Anti-Cytochrome P450 2E1 antibody (ab28146) at 1/2500 dilution

    Lane 1 : Tissue lysate prepared from normal murine liver
    Lane 2 : Tissue lysate prepared from normal murine liver
    Lane 3 : Tissue lysate prepared from normal murine liver
    Lane 4 : Tissue lysate prepared from normal murine liver

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat anti-rabbit IgG-HRP at 1/5000 dilution
    Developed using the ECL technique

    Predicted band size : 50-55 kDa
    Observed band size : 55 kDa (why is the actual band size different from the predicted?)


    Exposure time : 3 seconds

    Image courtesy of an anonymous Abreview.

    See Abreview

Anti-Cytochrome P450 2E1 antibody (ab28146)参考文献

This product has been referenced in:
  • Probert PM  et al. Utility of B-13 progenitor-derived hepatocytes in hepatotoxicity and genotoxicity studies. Toxicol Sci 137:350-70 (2014). ICC/IF ; Rat . Read more (PubMed: 24235770) »
  • Nixon BJ  et al. Mouse spermatocytes express CYP2E1 and respond to acrylamide exposure. PLoS One 9:e94904 (2014). ICC/IF ; Mouse . Read more (PubMed: 24788432) »

See all 19 Publications for this product

Product Wall

Application Western blot
Sample Pig Tissue lysate - other (Adipose tissue)
Loading amount 8 µg
Specification Adipose tissue
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Dec 18 2012

Thank you for kindly confirming these details as this enables us to closely monitor the quality of our products

We would be pleased to arrange a replacement product free of charge of ab28146 from a different lot. I am forwarding these detail...

Read More

Thank you for taking time to answer my questions. I am sorry to hear this antibody is not providing satisfactory results.

The details provided will enable us to investigate this case and will provide us with vital information for monitoring ...

Read More

I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

The details provided will enable us to inve...

Read More

Thank you for taking time to answer my questions.



Having reviewed the answers, I would like to offer some suggestions to help optimize the results from ab28146 Anti-Cytochrome P450 2E1 antibody.



1.I can suggest RIP...

Read More

Thank you for contacting Abcam’s scientific support team.

A replacement is available within 6 months of purchase if the antibody has failed for applications and species listed on the datasheet.

However, with some details of your...

Read More

Thank you for contacting us.

Both mentioned antibodies have been validated to react with rat species. At Abcam, our aim is to share all the information we have available from our products in the Website, to help customers decide the best opti...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - other (Liver)
Loading amount 10 µg
Specification Liver
Gel Running Conditions Non-reduced Non-Denaturing (Native) (12% SDS PAGE)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 Aug 01 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Rat Tissue sections (Liver)
Specification Liver
Fixative Acetone 10' at 4°C
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 20°C
Username

Abcam user community

Verified customer

提交于 Oct 12 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Rat Cell (liver sections)
Specification liver sections
Fixative Acetone
Blocking step BSA as blocking agent for 5 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 May 21 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"