Anti-Cytochrome P450 2C9抗体(ab4236)


  • 产品名称
    Anti-Cytochrome P450 2C9抗体
    参阅全部 Cytochrome P450 2C9 一抗
  • 描述
    兔多克隆抗体to Cytochrome P450 2C9
  • 宿主
  • 经测试应用
    适用于: IHC-P, WBmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Recombinant full length protein (Human) expressed in E. coli.

  • 常规说明

    The Cytochrome P450 (P450) superfamily of enzymes is one of three enzyme systems which metabolize the fatty acid arachadonic acid (AA) to regulators of vascular tone. P450 enzymes are monooxygenase enzymes which require several co-factors such as nicotinamide adenine dinucleotide phosphate (NADPH) and P450 reductase. There are over 200 known genes which encode P450s. Epoxygenases are those P450s which metabolize AA to epoxyeicosatrienoic acid (EETs) and omega-hydroxylases are those P450s which produce 19- and 20-hydroxyeicosatetraenoic acids (19- and 20-HETE). As well as fatty acid metabolism, P450s also metabolize many drugs and toxins. Cytochrome P450 3A4 is abundantly expressed in liver and small intestine and is inducible by barbiturates, glucocorticoids and rifampicin.



Our Abpromise guarantee covers the use of ab4236 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/1000. Predicted molecular weight: 59 kDa. Positive control 0.1 µg of recombinant human cytochrome P450 2


  • 功能
    Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. This enzyme contributes to the wide pharmacokinetics variability of the metabolism of drugs such as S-warfarin, diclofenac, phenytoin, tolbutamide and losartan.
  • 序列相似性
    Belongs to the cytochrome P450 family.
  • 细胞定位
    Endoplasmic reticulum membrane. Microsome membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • (R)-limonene 6-monooxygenase antibody
    • (S)-limonene 6-monooxygenase antibody
    • (S)-limonene 7-monooxygenase antibody
    • CP2C9_HUMAN antibody
    • CPC9 antibody
    • CYP2C antibody
    • CYP2C10 antibody
    • CYP2C9 antibody
    • CYPIIC9 antibody
    • cytochrome P-450 S-mephenytoin 4-hydroxylase antibody
    • Cytochrome P-450MP antibody
    • Cytochrome P450 2C9 antibody
    • Cytochrome P450 MP-4 antibody
    • Cytochrome P450 MP-8 antibody
    • Cytochrome P450 PB-1 antibody
    • Cytochrome P450, family 2, subfamily C, polypeptide 9 antibody
    • Cytochrome p4502C9 antibody
    • flavoprotein-linked monooxygenase antibody
    • MGC149605 antibody
    • MGC88320 antibody
    • microsomal monooxygenase antibody
    • OTTHUMP00000020135 antibody
    • P450 MP antibody
    • P450 PB 1 antibody
    • P450 PB1 antibody
    • P450IIC9 antibody
    • P450MP antibody
    • S mephenytoin 4 hydroxylase antibody
    • S-mephenytoin 4-hydroxylase antibody
    • xenobiotic monooxygenase antibody
    see all


  • IHC image of ab4236 staining in human liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab4236, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.


This product has been referenced in:
  • Guo R  et al. Chemical Cocktails Enable Hepatic Reprogramming of Mouse Fibroblasts with a Single Transcription Factor. Stem Cell Reports 9:499-512 (2017). Read more (PubMed: 28757167) »
  • Skrypnyk N  et al. PPARa activation can help prevent and treat non-small cell lung cancer. Cancer Res 74:621-31 (2014). Read more (PubMed: 24302581) »

See all 4 Publications for this product


Thank you for your reply and for sending your data. I agree that the results you have obtained are difficult to interpret. For that reason, I would be happy to offer you free of charge replacements, or a credit or refund. Please let me know how you wou...

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Thank you for contacting us.
Because ab4236 is a rabbit polyclonal antibody that was raised against a full length protein, you could use a full length recombinant Cytochrome P450 2C9 protein such as ab56998 for blocking. It is very difficult and e...

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Expiration date: May 26th, 2012

I am very pleased to hear you would like to accept our offer and test ab4236 in rat. This code will give you 1 free primary antibody before the expiration date. To redeem this offer, ple...

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Thank you for contacting Abcam. Please find attached the requested Certificate of Compliance

Abcam has not validated the combination of species/application used in this Abreview.
Western blot
Sheep Tissue lysate - whole (Liver)
Loading amount
80 µg
Gel Running Conditions
Reduced Denaturing (4-12% Bis-Tris Gel)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Mr. Joseph Statwick

Verified customer

提交于 Mar 09 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Western blot
Mouse Tissue lysate - whole (liver)
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing (4-20% gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

提交于 Dec 13 2010

Thank you for contacting us. For ab23677, LTA4 hydrolase, I suggest either a neutrophil or monocyte lysate. We carry THP1 lysate, ab7913, which is a human monocytic leukemia. For a negative control, LTA4 was detected in epidermal keratinocytes and ...

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