Anti-Cytochrome P450 2A6抗体(ab3570)

概述

  • 产品名称
    Anti-Cytochrome P450 2A6抗体
    参阅全部 Cytochrome P450 2A6 一抗
  • 描述
    兔多克隆抗体to Cytochrome P450 2A6
  • 经测试应用
    适用于: IHC-P, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Other Immunogen Type (His-tag) corresponding to Human Cytochrome P450 2A6. Purified, His-tagged, full length human P450 2A6 fusion protein.

  • 阳性对照
    • Rat liver lysate.

性能

应用

Our Abpromise guarantee covers the use of ab3570 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use at an assay dependent concentration.
WB 1/2000. Detects a band of approximately 49 kDa (predicted molecular weight: 57 kDa).

This antibody detects an ~49 kDa protein representing P450 2A6 from rat liver extract.

靶标

  • 功能
    Exhibits a high coumarin 7-hydroxylase activity. Can act in the hydroxylation of the anti-cancer drugs cyclophosphamide and ifosphamide. Competent in the metabolic activation of aflatoxin B1. Constitutes the major nicotine C-oxidase. Possesses low phenacetin O-deethylation activity.
  • 组织特异性
    Liver.
  • 序列相似性
    Belongs to the cytochrome P450 family.
  • 细胞定位
    Endoplasmic reticulum membrane. Microsome membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Coumarin 7 hydroxylase antibody
    • Coumarin 7-hydroxylase antibody
    • CP2A6_HUMAN antibody
    • CPA6 antibody
    • CYP2A antibody
    • CYP2A3 antibody
    • CYP2A6 antibody
    • CYPIIA6 antibody
    • Cytochrome P450 2A6 antibody
    • Cytochrome P450 IIA3 antibody
    • Cytochrome P450 subfamily IIA (phenobarbital inducible) polypeptide 6 antibody
    • Cytochrome P450(I) antibody
    • Cytochrome P450, family 2 subfamily A polypeptide 6 antibody
    • Flavoprotein linked monooxygenase antibody
    • P450C2A antibody
    • P450PB antibody
    • Xenobiotic monooxygenase antibody
    see all

图片



  • Predicted band size : 57 kDa

    ab3570 at a dilution of 1/2000 staining cytochrome P450 2A6 in rat liver lysate by Western blot.

文献

ab3570 has not yet been referenced specifically in any publications.

客户评价及客户问答

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Liver)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate buffet, pH6
Permeabilization
No
Specification
Liver
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

提交于 Jul 06 2017

Thank you for your response. While I cannot retroactively apply a discount to the order you have placed, the code will entitle you to xx off of one primary antibody for a future purchase. The code must be used on or before 10 October 2012. I woul...

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Thank you for contacting us. The recommended dilution of 1:150 is a recommendation which we make to our customers based on our experiments or those of supplier, collaborator or customer input. Although we cannot know exactly why certain products req...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (liver)
Loading amount
30 µg
Specification
liver
Gel Running Conditions
Reduced Denaturing (4-20% gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Dec 13 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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