This antibody gave a positive signal in both Human Lung and Placenta tissue lysates.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.40 Preservative: 0.02% Sodium azide Constituent: PBS Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 73 kDa (predicted molecular weight: 58 kDa).
Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.
Cytochrome P450 family 1 subfamily A polypeptide 1 antibody
Cytochrome P450 form 6 antibody
Cytochrome P450 subfamily I (aromatic compound inducible) polypeptide 1 antibody
Cytochrome P450-C antibody
Cytochrome P450-P1 antibody
Flavoprotein-linked monooxygenase antibody
Microsomal monooxygenase antibody
P1 450 antibody
P450 C antibody
P450 form 6 antibody
P450 P1 antibody
Xenobiotic monooxygenase antibody
Western blot - Anti-Cytochrome P450 1A1 antibody (ab124295)
All lanes : Anti-Cytochrome P450 1A1 antibody (ab124295) at 1 µg/ml
Lane 1 : Lung (Human) Tissue Lysate Lane 2 : Human placenta tissue lysate - total protein (ab29745) Lane 3 : Lung (Human) Tissue Lysate with Immunising peptide at 1 µg/ml Lane 4 : Human placenta tissue lysate - total protein (ab29745) with Immunising peptide at 1 µg/ml
Lysates/proteins at 10 µg per lane.
Secondary All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
This blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab124295 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.