Anti-Cytochrome P450 1A1 + 1A2抗体[C4.0+] (ab81816)

概述

应用

Our Abpromise guarantee covers the use of ab81816 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000. Predicted molecular weight: 12 kDa. 5 uL/10 mL of TBS (1:2000 dilution) containing 5% non-fat milk for overnight incubation is necessary for ECL method. We recommend a 1:5000 dilution of HRP conjugated anti-mouse secondary antibody in TBS containing 5% non-fat milk for 1 hour incubation.

靶标

  • 相关性Cytochrome P450 oxidase (commonly abbreviated CYP) is a generic term for a large number of related, but distinct, oxidative enzymes important in vertebrate physiology. The cytochrome P450 mixed-function monooxygenase system is probably the most important element of Phase I metabolism in mammals. P450s are membrane-bound, either in the inner membrane of mitochondria or in the endoplasmic reticulum of cells where they metabolise thousands of endogenous and exogenous compounds. In the liver, these substrates include toxins, drugs, and other unneeded and potentially harmful molecules. Humans have 18 families of cytochrome P450 genes and 43 subfamilies; the CYP1 family is involved in drug metabolism and includes 3 subfamilies, 3 genes and 1 pseudogene.
  • 细胞定位Endoplasmic reticulum
  • 数据库链接
  • 别名
    • Aryl hydrocarbon hydroxylase antibody
    • CP11 antibody
    • CP12 antibody
    • CYP1A1 antibody
    • CYP1A2 antibody
    • CYPIA1 antibody
    • CYPIA2 antibody
    • Cytochrome P450 family 1 subfamily A polypeptide 1 antibody
    • Cytochrome P450 family 1 subfamily A polypeptide 2 antibody
    • Cytochrome P450 subfamily I aromatic compound inducible polypeptide 1 antibody
    • Cytochrome P450 subfamily I aromatic compound inducible polypeptide 2 antibody
    • P1 450 antibody
    • P3 450 antibody
    • P450 form 4 antibody
    • P450 form 6 antibody
    • P450 P1 antibody
    • P450 P3 antibody
    see all

Anti-Cytochrome P450 1A1 + 1A2 antibody [C4.0+] 图像

  • All lanes : Anti-Cytochrome P450 1A1 + 1A2 antibody [C4.0+] (ab81816)

    Lane 1 : Male Sprague Dawley rat microsomes treated with 3-MC (25 mg/kg, single injection, i.p.)
    Lane 2 : Male Sprague Dawley rat microsomes treated with corn oil (2 mL/kg/day, for 3 days, i.p.)

    Lysates/proteins at 10 µg per lane.


    Predicted band size : 12 kDa

Anti-Cytochrome P450 1A1 + 1A2 antibody [C4.0+] (ab81816)参考文献

This product has been referenced in:
  • Floreani M  et al. Severe liver cirrhosis markedly reduces AhR-mediated induction of cytochrome P450 in rats by decreasing the transcription of target genes. PLoS One 8:e61983 (2013). WB ; Rat . Read more (PubMed: 23626760) »

See 1 Publication for this product

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Thank you for contacting us.

I heard back from the supplying lab with the following information:

All we can say is that it is a sequence common to both CYP1A1 and CYP1A2. Unfortunately, nofurther specific information is available. ...

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