RabMAb

Anti-Cytochrome P450 17A1抗体[EPR6293] (ab125022)

概述

应用

Our Abpromise guarantee covers the use of ab125022 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Detects a band of approximately 55 kDa (predicted molecular weight: 57 kDa).
IP 1/10 - 1/100.
Flow Cyt Use at an assay dependent concentration.
ICC/IF 1/100 - 1/250.
  • 应用说明Is unsuitable for IHC-P.
  • 靶标

    • 功能Conversion of pregnenolone and progesterone to their 17-alpha-hydroxylated products and subsequently to dehydroepiandrosterone (DHEA) and androstenedione. Catalyzes both the 17-alpha-hydroxylation and the 17,20-lyase reaction. Involved in sexual development during fetal life and at puberty.
    • 通路Lipid metabolism; steroid biosynthesis.
    • 疾病相关Defects in CYP17A1 are the cause of adrenal hyperplasia type 5 (AH5) [MIM:202110]. AH5 is a form of congenital adrenal hyperplasia, a common recessive disease due to defective synthesis of cortisol. Congenital adrenal hyperplasia is characterized by androgen excess leading to ambiguous genitalia in affected females, rapid somatic growth during childhood in both sexes with premature closure of the epiphyses and short adult stature. Four clinical types: "salt wasting" (SW, the most severe type), "simple virilizing" (SV, less severely affected patients), with normal aldosterone biosynthesis, "non-classic form" or late onset (NC or LOAH), and "cryptic" (asymptomatic).
    • 序列相似性Belongs to the cytochrome P450 family.
    • 翻译后修饰Phosphorylation is necessary for 17,20-lyase, but not for 17-alpha-hydroxylase activity.
    • 细胞定位Membrane.
    • Information by UniProt
    • 数据库链接
    • 别名
      • 20 lyase antibody
      • CP17A_HUMAN antibody
      • CPT7 antibody
      • CYP17 antibody
      • CYP17A1 antibody
      • CYPXVII antibody
      • Cytochrome P450 17A1 antibody
      • Cytochrome P450 family 17 antibody
      • Cytochrome P450 family 17 subfamily A polypeptide 1 antibody
      • Cytochrome p450 subfamily XVII (steroid 17 alpha hydroxylase) adrenal hyperplasia antibody
      • Cytochrome p450 XVIIA1 antibody
      • Cytochrome P450-C17 antibody
      • Cytochrome P450c17 antibody
      • OTTHUMP00000020382 antibody
      • P450 C17 antibody
      • P450c17 antibody
      • S17AH antibody
      • Steroid 17 alpha hydroxylase/17,20 lyase antibody
      • Steroid 17 alpha monooxygenase antibody
      • Steroid 17-alpha-hydroxylase/17 antibody
      • Steroid 17-alpha-monooxygenase antibody
      see all

    Anti-Cytochrome P450 17A1 antibody [EPR6293] 图像

    • ab125022, at 1/100 dilution, staining Cytochrome P450 17A1 in Hela cells by Immunofluorescence.
    • All lanes : Anti-Cytochrome P450 17A1 antibody [EPR6293] (ab125022) at 1/1000 dilution

      Lane 1 : Human adrenal gland lysate
      Lane 2 : SK-OV-3 lysate
      Lane 3 : HeLa lysate
      Lane 4 : Jurkat lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 57 kDa
      Observed band size : 55 kDa (why is the actual band size different from the predicted?)
    • Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling Cytochrome P450 17A1 with purified ab125022 at 1/220 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

    Anti-Cytochrome P450 17A1 antibody [EPR6293] (ab125022)参考文献

    This product has been referenced in:
    • Wu S  et al. Obesity-induced infertility and hyperandrogenism are corrected by deletion of the insulin receptor in the ovarian theca cell. Diabetes 63:1270-82 (2014). Mouse . Read more (PubMed: 24379345) »
    • Dowling AR  et al. Genetic factors modulate the impact of pubertal androgen excess on insulin sensitivity and fertility. PLoS One 8:e79849 (2013). WB ; Mouse . Read more (PubMed: 24278193) »

    See all 2 Publications for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (H1299 Lung cancer cell line)
    Permeabilization Yes - 0.1% v/v TritonX-100
    Specification H1299 Lung cancer cell line
    Blocking step BSA as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: RT°C
    Fixative Paraformaldehyde
    Username

    Dr. Dimitra Kalamida

    Verified customer

    提交于 May 31 2016

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"