Anti-Cytochrome P450 17A1抗体(ab48019)


  • 产品名称Anti-Cytochrome P450 17A1抗体
    参阅全部 Cytochrome P450 17A1 一抗
  • 描述
    山羊多克隆抗体to Cytochrome P450 17A1
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Horse, Hamster, Cat, Dog, Chimpanzee, Baboon, Cynomolgus Monkey, Rhesus monkey
  • 免疫原

    Synthetic peptide:


    , corresponding to internal sequence amino acids 134-144 of Human Cytochrome P450 17A1.

  • 阳性对照
    • Human ovary lysates.



Our Abpromise guarantee covers the use of ab48019 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 57 kDa).
IHC-P Use a concentration of 3 - 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.


  • 功能Conversion of pregnenolone and progesterone to their 17-alpha-hydroxylated products and subsequently to dehydroepiandrosterone (DHEA) and androstenedione. Catalyzes both the 17-alpha-hydroxylation and the 17,20-lyase reaction. Involved in sexual development during fetal life and at puberty.
  • 通路Lipid metabolism; steroid biosynthesis.
  • 疾病相关Defects in CYP17A1 are the cause of adrenal hyperplasia type 5 (AH5) [MIM:202110]. AH5 is a form of congenital adrenal hyperplasia, a common recessive disease due to defective synthesis of cortisol. Congenital adrenal hyperplasia is characterized by androgen excess leading to ambiguous genitalia in affected females, rapid somatic growth during childhood in both sexes with premature closure of the epiphyses and short adult stature. Four clinical types: "salt wasting" (SW, the most severe type), "simple virilizing" (SV, less severely affected patients), with normal aldosterone biosynthesis, "non-classic form" or late onset (NC or LOAH), and "cryptic" (asymptomatic).
  • 序列相似性Belongs to the cytochrome P450 family.
  • 翻译后修饰Phosphorylation is necessary for 17,20-lyase, but not for 17-alpha-hydroxylase activity.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 20 lyase antibody
    • CP17A_HUMAN antibody
    • CPT7 antibody
    • CYP17 antibody
    • CYP17A1 antibody
    • CYPXVII antibody
    • Cytochrome P450 17A1 antibody
    • Cytochrome P450 family 17 antibody
    • Cytochrome P450 family 17 subfamily A polypeptide 1 antibody
    • Cytochrome p450 subfamily XVII (steroid 17 alpha hydroxylase) adrenal hyperplasia antibody
    • Cytochrome p450 XVIIA1 antibody
    • Cytochrome P450-C17 antibody
    • Cytochrome P450c17 antibody
    • OTTHUMP00000020382 antibody
    • P450 C17 antibody
    • P450c17 antibody
    • S17AH antibody
    • Steroid 17 alpha hydroxylase/17,20 lyase antibody
    • Steroid 17 alpha monooxygenase antibody
    • Steroid 17-alpha-hydroxylase/17 antibody
    • Steroid 17-alpha-monooxygenase antibody
    see all

Anti-Cytochrome P450 17A1 antibody 图像

  • Anti-Cytochrome P450 17A1 antibody (ab48019) at 0.3 µg/ml + 35µg Human ovary lysates in RIPA buffer

    anti-goat-HRP at a 1/3000 dilution
    developed using the ECL technique

    Predicted band size : 57 kDa
    Observed band size : 60 kDa (why is the actual band size different from the predicted?)
  • ab48019 (3.8µg/ml) staining of paraffin embedded Human Adrenal Gland shows strong granular staining of the cytoplasm in medulla cells. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Anti-Cytochrome P450 17A1 antibody (ab48019)参考文献

ab48019 has not yet been referenced specifically in any publications.

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