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Our Abpromise guarantee covers the use of ab16076 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 12 kDa.|
Western blot of human Cytochrome C from 293T cells (divided into two fractions: Lane 1- cytoplasmic, Lane 2-nuclear) using anti Cytochrome c antibody (ab16076) at 1µg/mL.
Method: Cell extracts from 293T cells (2x106) were separated into cytoplasmic and nuclear fraction, resolved by SDS-PAGE under reducing conditions, transferred to nitrocellulose and probed with the monoclonal anti-cytochrome c antibody (ab16076) at 1µg/ml. Proteins were visualized using a peroxidase-conjugated antibody to mouse IgG and a chemiluminescence detection system.