Anti-Cytochrome C抗体[SJL2-4(apo)] (ab16076)

概述

  • 产品名称Anti-Cytochrome C抗体[SJL2-4(apo)]
    参阅全部 Cytochrome C 一抗
  • 描述
    小鼠单克隆抗体[SJL2-4(apo)] to Cytochrome C
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Sheep, Goat, Human, Monkey
  • 免疫原

    Recombinant full length protein (Horse).

  • 阳性对照
    • 293T cells

应用

Our Abpromise guarantee covers the use of ab16076 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 12 kDa.

靶标

  • 功能Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain.
    Plays a role in apoptosis. Suppression of the anti-apoptotic members or activation of the pro-apoptotic members of the Bcl-2 family leads to altered mitochondrial membrane permeability resulting in release of cytochrome c into the cytosol. Binding of cytochrome c to Apaf-1 triggers the activation of caspase-9, which then accelerates apoptosis by activating other caspases.
  • 疾病相关Defects in CYCS are the cause of thrombocytopenia type 4 (THC4) [MIM:612004]; also known as autosomal dominant thrombocytopenia type 4. Thrombocytopenia is the presence of relatively few platelets in blood. THC4 is a non-syndromic form of thrombocytopenia. Clinical manifestations of thrombocytopenia are absent or mild. THC4 may be caused by dysregulated platelet formation.
  • 序列相似性Belongs to the cytochrome c family.
  • 翻译后修饰Binds 1 heme group per subunit.
  • 细胞定位Mitochondrion matrix.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CYC antibody
    • CYC_HUMAN antibody
    • CYCS antibody
    • Cytochrome c antibody
    • Cytochrome c somatic antibody
    • HCS antibody
    • THC4 antibody
    see all

Anti-Cytochrome C antibody [SJL2-4(apo)] 图像

  • Western blot of human Cytochrome C from 293T cells (divided into two fractions: Lane 1- cytoplasmic, Lane 2-nuclear) using anti Cytochrome c antibody (ab16076) at 1µg/mL.

    Method: Cell extracts from 293T cells (2x106) were separated into cytoplasmic and nuclear fraction, resolved by SDS-PAGE under reducing conditions, transferred to nitrocellulose and probed with the monoclonal anti-cytochrome c antibody (ab16076) at 1µg/ml. Proteins were visualized using a peroxidase-conjugated antibody to mouse IgG and a chemiluminescence detection system.

Anti-Cytochrome C antibody [SJL2-4(apo)] (ab16076)参考文献

This product has been referenced in:
  • Corradin G  et al. Differential effects of two anti-apo-cytochrome c-specific monoclonal antibodies on the function of apo-cytochrome c-specific murine T cell clones. J Immunol 133:2915-9 (1984). Read more (PubMed: 6208267) »

See 1 Publication for this product

Product Wall

Thank you for your enquiry. This antibody should work well in IP applications. As decribed in details in the literature specific reference of this product (Differential effects of two anti-apo-cytochrome c-specific monoclonal antibodies on the function...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"