Anti-Cytochrome C抗体[37BA11] (ab110325)

Abreviews (3)Q&A (1)Specific References (51)

概述

  • 产品名称
    Anti-Cytochrome C抗体[37BA11]
    参阅全部 Cytochrome C 一抗
  • 描述
    小鼠单克隆抗体[37BA11] to Cytochrome C
  • 宿主
    Mouse
  • 经测试应用
    适用于: WB, ICC/IF, Flow Cyt, In-Cell ELISAmore details
  • 种属反应性
    与反应: Mouse, Rat, Cow, Human, Caenorhabditis elegans
  • 免疫原

    Bovine heart Cytochrome C

  • 阳性对照
    • Isolated mitochondria from Human, Bovine, Rat and Mouse heart; Human fibroblasts; HeLa cells.
  • 常规说明

    This antibody clone is manufactured by Abcam.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

性能

应用

Our Abpromise guarantee covers the use of ab110325 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.25 - 1 µg/ml. Predicted molecular weight: 12 kDa.
ICC/IF Use a concentration of 0.5 - 2 µg/ml. Heat induced antigen retrieval in 0.1 M Tris-HCl, 5% urea, pH 9.5 for 5 min at 95°C improves signal.
Flow Cyt Use a concentration of 1 µg/ml.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

 
In-Cell ELISA Use a concentration of 4 µg/ml.

靶标

  • 功能
    Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain.
    Plays a role in apoptosis. Suppression of the anti-apoptotic members or activation of the pro-apoptotic members of the Bcl-2 family leads to altered mitochondrial membrane permeability resulting in release of cytochrome c into the cytosol. Binding of cytochrome c to Apaf-1 triggers the activation of caspase-9, which then accelerates apoptosis by activating other caspases.
  • 疾病相关
    Defects in CYCS are the cause of thrombocytopenia type 4 (THC4) [MIM:612004]; also known as autosomal dominant thrombocytopenia type 4. Thrombocytopenia is the presence of relatively few platelets in blood. THC4 is a non-syndromic form of thrombocytopenia. Clinical manifestations of thrombocytopenia are absent or mild. THC4 may be caused by dysregulated platelet formation.
  • 序列相似性
    Belongs to the cytochrome c family.
  • 翻译后修饰
    Binds 1 heme group per subunit.
  • 细胞定位
    Mitochondrion matrix.
  • Information by UniProt
  • 数据库链接
    see all
  • 别名
    • CYC antibody
    • CYC_HUMAN antibody
    • CYCS antibody
    • Cytochrome c antibody
    • Cytochrome c somatic antibody
    • HCS antibody
    • THC4 antibody
    see all

图片

  • Immunocytochemistry/ Immunofluorescence - Anti-Cytochrome C antibody [37BA11] (ab110325)
    Immunocytochemistry/ Immunofluorescence - Anti-Cytochrome C antibody [37BA11] (ab110325)This image is courtesy of an anonymous Abreview

    ab110325 staining Cytochrome C in MDA MB 231 cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde, permeabilized with 1% Triton X-100 and blocked with 10% BSA for 1 hour at 21°C. Samples were incubated with primary antibody (1/100) for 1 hour. An undiluted DyLight® 550-conjugated goat anti-mouse IgG polyclonal was used as the secondary antibody.

    See Abreview

  • Immunocytochemistry/ Immunofluorescence - Anti-Cytochrome C antibody [37BA11] (ab110325)
    Immunocytochemistry/ Immunofluorescence - Anti-Cytochrome C antibody [37BA11] (ab110325)
    ab110325 staining cytochrome C in MCF7 cells treated with 15-Deoxy-delta12,14-prostaglandin J2 (ab141717), by ICC/IF. Expression of cytochrome C changes from mitochondrial puncta to a difuse staining pattern with increased concentration of 15-Deoxy-delta12,14-prostaglandin J2, as described in literature.
    The cells were incubated at 37°C for 24 hours in media containing different concentrations of ab141717 (15-Deoxy-delta12,14-prostaglandin J2) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab110325 (10 μg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 anti-mouse polyclonal antibody (ab96879) at 1/250 dilution was used as the secondary antibody. Nuclei (blue) were counterstained with DAPI and membrane is was stained using WGA (red).
  • Western blot - Anti-Cytochrome C antibody [37BA11] (ab110325)
    Western blot - Anti-Cytochrome C antibody [37BA11] (ab110325)
    All lanes : Anti-Cytochrome C antibody [37BA11] (ab110325) at 1 µg/ml

    Lane 1 : Isolated mitochondria from Human heart at 5 µg
    Lane 2 : Isolated mitochondria from Bovine heart at 1 µg
    Lane 3 : Isolated mitochondria from Rat heart at 10 µg
    Lane 4 : Isolated mitochondria from Mouse heart at 10 µg

    Predicted band size: 12 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Cytochrome C antibody [37BA11] (ab110325)
    Immunocytochemistry/ Immunofluorescence - Anti-Cytochrome C antibody [37BA11] (ab110325)
    Immunocytochemistry analysis using ab110325 at 2µg/ml staining Cytochrome C in Human fibroblasts (fixed and permeabilized), followed by a fluorescent goat-anti-mouse IgG.
  • Flow Cytometry - Anti-Cytochrome C antibody [37BA11] (ab110325)
    Flow Cytometry - Anti-Cytochrome C antibody [37BA11] (ab110325)
    Flow cytometric analysis using ab110325 at 1µg/ml staining Cytochrome C in HeLa cells (blue). Isotype control antibody (red).

文献

This product has been referenced in:
  • Monterisi S  et al. PDE2A2 regulates mitochondria morphology and apoptotic cell death via local modulation of cAMP/PKA signalling. Elife 6:N/A (2017). IHC-P . Read more (PubMed: 28463107) »
  • Guo J  et al. Inhibition of Fatty Acid Synthase Reduces Blastocyst Hatching through Regulation of the AKT Pathway in Pigs. PLoS One 12:e0170624 (2017). Read more (PubMed: 28107461) »

See all 51 Publications for this product

发表研究结果有使用 ab110325？请让我们知道，以便我们可以引用本数据表中的参考文章。

客户评价及客户问答

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4 reviews or Q&A

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Western blot abreview for Anti-Cytochrome C antibody [37BA11]

 Excellent
Abreviews
Application
Western blot
Sample
Guinea pig Cell lysate - other (Liver, mitochondrial fraction)
Gel Running Conditions
Reduced Denaturing (12.5 % acrylamide)
Loading amount
60 µg
Specification
Liver, mitochondrial fraction
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 7.5% · Temperature: 22°C
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Abcam user community

Verified customer

提交于 May 12 2015

Immunocytochemistry/ Immunofluorescence abreview for Anti-Cytochrome C antibody [37BA11]

 Excellent
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 21°C
Sample
Human Cell (MDA MB 231 cells)
Specification
MDA MB 231 cells
Permeabilization
Yes - 1% Triton
Fixative
Formaldehyde
Read More
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Abcam user community

Verified customer

提交于 Feb 19 2015

Western blot abreview for Anti-Cytochrome C antibody [37BA11]

 Excellent
Abreviews
Application
Western blot
Loading amount
10 µg
Gel Running Conditions
Reduced Denaturing (15)
Sample
Human Cell lysate - other (fractionated cells (nuclear, cytosolic, mitochondr)
Specification
fractionated cells (nuclear, cytosolic, mitochondr
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 18°C
Read More
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Abcam user community

Verified customer

提交于 Nov 28 2014

I want to measure just the cytochrome C secreted from the mitochondria, but not in the mitochondria itself. Is there a lysis buffer or protocol to follow that will do this efficiently?

Thank you for your patience in awaiting our reply!

We have 2 products that perfectly fit your experiment:

http://www.abcam.com/Cell-Fractionation-Kit-Standard-ab109719.html

http://www.abcam.com/Cell-Fractionation-Kit-HT-ab...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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