概述

  • 产品名称Anti-Cytochrome b5抗体
    参阅全部 Cytochrome b5 一抗
  • 描述
    兔多克隆抗体to Cytochrome b5
  • 经测试应用适用于: ELISA, WB, ICC/IF, IHC-Pmore details
  • 种属反应性
    与反应: Rat, Human
    预测可用于: Mouse
  • 免疫原

    Synthetic peptide derived from an internal region of Human Cytochrome b5.

  • 阳性对照
    • NIH/3T3 cells; extracts from A549 cells.

性能

应用

Our Abpromise guarantee covers the use of ab69801 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/5000.
WB 1/500 - 1/1000. Detects a band of approximately 15 kDa (predicted molecular weight: 15 kDa).
ICC/IF 1/500 - 1/1000.
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能Cytochrome b5 is a membrane bound hemoprotein which function as an electron carrier for several membrane bound oxygenases.
  • 疾病相关Defects in CYB5A are the cause of methemoglobinemia CYB5A-related (METHB-CYB5A) [MIM:250790]. A form of methemoglobinemia, a hematologic disease characterized by the presence of excessive amounts of methemoglobin in blood cells, resulting in decreased oxygen carrying capacity of the blood, cyanosis and hypoxia.
  • 序列相似性Belongs to the cytochrome b5 family.
    Contains 1 cytochrome b5 heme-binding domain.
  • 细胞定位Cytoplasm and Endoplasmic reticulum membrane. Microsome membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CYB 5 antibody
    • CYB 5A antibody
    • CYB5 antibody
    • CYB5_HUMAN antibody
    • Cyb5a antibody
    • Cytochrome b 5 antibody
    • Cytochrome b5 (microsomal) antibody
    • Cytochrome b5 antibody
    • Cytochrome b5 type A (microsomal) antibody
    • Cytochrome b5 type A antibody
    • MCB 5 antibody
    • MCB5 antibody
    • Microsomal cytochrome b5 antibody
    • Microsomal cytochrome b5 type A antibody
    • Type 1 cyt b5 antibody
    see all

Anti-Cytochrome b5 antibody 图像

  • All lanes : Anti-Cytochrome b5 antibody (ab69801) at 1/500 dilution

    Lane 1 : extracts from A549 cells
    Lane 2 : extracts from A549 cells with immunizing peptide at 10 µg

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 15 kDa
    Observed band size : 15 kDa
  • Left: NIH/3T3 cells stained with ab69801 at 1/500 dilution; Right: the antibody was pre-incubated with the immunizing peptide.
  • IHC image of ab69801 staining in human normal liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab69801, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-Cytochrome b5 antibody (ab69801)参考文献

This product has been referenced in:
  • Stark K  et al. CYB5A polymorphism increases androgens and reduces risk of rheumatoid arthritis in women. Arthritis Res Ther 17:56 (2015). IHC-P ; Human . Read more (PubMed: 25890314) »
  • Chen K  et al. AMP activated protein kinase is indispensable for myocardial adaptation to caloric restriction in mice. PLoS One 8:e59682 (2013). WB ; Mouse . Read more (PubMed: 23527250) »

See all 6 Publications for this product

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