Anti-Cytochrome b245 Light Chain抗体(ab75941)

概述

  • 产品名称
    Anti-Cytochrome b245 Light Chain抗体
    参阅全部 Cytochrome b245 Light Chain 一抗
  • 描述
    兔多克隆抗体to Cytochrome b245 Light Chain
  • 经测试应用
    适用于: WB, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
    预测可用于: Cow, Pig
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 100 to the C-terminus Cytochrome b245 Light Chain.

    (Peptide available as ab86646.)

  • 阳性对照
    • This antibody gave a positive signal in the following lysates: HepG2 Whole Cell, Mouse Pancreas Tissue, Mouse Spleen Tissue. This antibody gave a positive result in IHC in the following FFPE tissue: Human normal lung.

性能

应用

Our Abpromise guarantee covers the use of ab75941 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 19, 21 kDa (predicted molecular weight: 21 kDa).
IHC-P Use a concentration of 1 µg/ml.
ICC/IF Use a concentration of 5 µg/ml.

靶标

  • 相关性
    Cytochrome b245 Light Chain/p22phox is a critical component of the membrane-bound oxidase complex that generates superoxide. It complexes with NOX3 to form a functional NADPH oxidase.
  • 细胞定位
    Cell Membrane
  • 数据库链接
  • 别名
    • CYBA antibody
    • Cytochrome b 245 alpha polypeptide antibody
    • Cytochrome b 245 light chain antibody
    • Cytochrome b alpha polypeptide antibody
    • Cytochrome b(558) alpha chain antibody
    • Cytochrome b(558) alpha subunit antibody
    • Cytochrome b558 subunit alpha antibody
    • Flavocytochrome b 558 alpha polypeptide antibody
    • Neutrophil cytochrome b 22 kDa polypeptide antibody
    • p22 phagocyte B-cytochrome antibody
    • p22-phox antibody
    • p22phox antibody
    • Superoxide-generating NADPH oxidase light chain subunit antibody
    see all

Anti-Cytochrome b245 Light Chain antibody 图像

  • All lanes : Anti-Cytochrome b245 Light Chain antibody (ab75941) at 1 µg/ml

    Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 2 : Pancreas (Mouse) Tissue Lysate
    Lane 3 : Spleen (Mouse) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 21 kDa
    Observed band size : 21 kDa
    Additional bands at : 19 kDa (possible cleavage fragment),40 kDa. We are unsure as to the identity of these extra bands.We hypothesize that the 21 kDa band represents the pro-form of Cytochrome b245 Light Chain, and the 19 kDa band represents the mature form.
  • ICC/IF image of ab75941 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab75941, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) HeLa cells at 5µg/ml, and in 4% PFA fixed (10 min) HeLa cells at 5µg/ml.
  • Immunohistochemical analyses of p22phox using ab75941 (1/200) in normal adrenocortical tissue (b-c) and adrenal aldosterone-producing adenoma, (e-f) p22phox presents a gradient of expression in the entire adrenal cortex (100x), and its localization was restricted to the cell membrane (400x). Sections were incubated with primary antibody at 4°C overnight and with a HRP-conjugated secondary antibody for 30 minutes at 37°C.

  • IHC image of Cytochrome b245 Light Chain staining in Human normal lung formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab75941, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-Cytochrome b245 Light Chain antibody (ab75941)参考文献

This product has been referenced in:
  • Geng X  et al. Role of Nox2 and p22phox in Persistent Postoperative Hypertension in Aldosterone-Producing Adenoma Patients after Adrenalectomy. Int J Endocrinol 2016:2395634 (2016). WB, IHC-P ; Human . Read more (PubMed: 27057164) »
  • Wilson C  et al. Contribution of NADPH oxidase to the establishment of hippocampal neuronal polarity in culture. J Cell Sci 128:2989-95 (2015). Read more (PubMed: 26101350) »

See all 5 Publications for this product

Product Wall

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HEK293T)
Permeabilization
Yes - 0.1% Triton X-100
Specification
HEK293T
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

提交于 Jul 14 2017

Application
Western blot
Sample
Rat Tissue lysate - whole (Skeletal muscle)
Gel Running Conditions
Reduced Denaturing (15%)
Loading amount
20 µg
Specification
Skeletal muscle
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Feb 02 2016

Application
Western blot
Sample
Mouse Tissue lysate - whole (Skeletal muscle)
Gel Running Conditions
Reduced Denaturing (15%)
Loading amount
20 µg
Specification
Skeletal muscle
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Feb 02 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (skeletal muscle)
Loading amount
50 µg
Specification
skeletal muscle
Gel Running Conditions
Reduced Denaturing (15% gel)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Dr. G.K Sakellariou

Verified customer

提交于 Sep 28 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Single isolated FDB skeletal muscle fibres)
Specification
Single isolated FDB skeletal muscle fibres
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% Triton X-100 (for 10 hours)
Blocking step
BSA as blocking agent for 10 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
Username

Dr. G.K Sakellariou

Verified customer

提交于 Sep 25 2012

Vielen Dank für Ihre Anfrage. Gegen die genannten NADPH-Oxidase-Komponenten haben wir folgende Antikörper bzw. Proteine in unserem Katalog: Click here (or use the following: http://www.abcam.com/index.html?datasheet=109366). Click here (or use the...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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