Anti-Cysteine Dioxygenase Type 1抗体(ab53436)

概述

  • 产品名称Anti-Cysteine Dioxygenase Type 1抗体
    参阅全部 Cysteine Dioxygenase Type 1 一抗
  • 描述
    兔多克隆抗体to Cysteine Dioxygenase Type 1
  • 经测试应用适用于: ICC/IF, WB, IP, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Recombinant full length protein (Rat)

性能

应用

Our Abpromise guarantee covers the use of ab53436 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 1 µg/ml.
WB 1/2000. Predicted molecular weight: 23 kDa.
IP Use at an assay dependent concentration.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能Initiates several important metabolic pathways related to pyruvate and several sulfurate compounds including sulfate, hypotaurine and taurine. Critical regulator of cellular cysteine concentrations. Has an important role in maintaining the hepatic concentation of intracellular free cysteine within a proper narrow range.
  • 组织特异性Highly expressed in liver and placenta. Low expression in heart, brain and pancreas. Also detected in hepatoblastoma HepG2 cells.
  • 通路Organosulfur biosynthesis; taurine biosynthesis; hypotaurine from L-cysteine: step 1/2.
  • 序列相似性Belongs to the cysteine dioxygenase family.
  • 翻译后修饰The thioether cross-link between Cys-93 and Tyr-157 plays a structural role through stabilizing the Fe(2+) ion, and prevents the production of highly damaging free hydroxyl radicals by holding the oxygen radical via hydroxyl hydrogen.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CDO 1 antibody
    • CDO antibody
    • CDO I antibody
    • CDO-I antibody
    • Cdo1 antibody
    • CDO1_HUMAN antibody
    • CDOI antibody
    • Cysteine dioxygenase type 1 antibody
    • Cysteine dioxygenase type I antibody
    • Cytosolic cysteine dioxygenase antibody
    see all

Anti-Cysteine Dioxygenase Type 1 antibody 图像

  • ICC/IF image of ab53436 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53436, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • IHC image of ab53436 staining in human normal liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab53436, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-Cysteine Dioxygenase Type 1 antibody (ab53436)参考文献

This product has been referenced in:
  • Prabhu A  et al. Cysteine catabolism: a novel metabolic pathway contributing to glioblastoma growth. Cancer Res 74:787-96 (2014). WB ; Human . Read more (PubMed: 24351290) »
  • Lee SD  et al. TonEBP stimulates multiple cellular pathways for adaptation to hypertonic stress: organic osmolyte-dependent and -independent pathways. Am J Physiol Renal Physiol 300:F707-15 (2011). WB ; Mouse . Read more (PubMed: 21209002) »

See all 2 Publications for this product

Product Wall

Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 50 µg
Gel Running Conditions Reduced Denaturing (12% gel)
Sample Mouse Tissue lysate - other (liver and kidney)
Specification liver and kidney
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Dr. Hua Jiang

Verified customer

提交于 Jun 17 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Small Cell Lung Cancer)
Loading amount 15 µg
Specification Small Cell Lung Cancer
Treatment CDO1 Overexpressed
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

提交于 Oct 15 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"