Anti-Cystatin C抗体[EPR4413] (ab109508)


  • 产品名称Anti-Cystatin C抗体[EPR4413]
    参阅全部 Cystatin C 一抗
  • 描述
    兔单克隆抗体[EPR4413] to Cystatin C
  • 经测试应用适用于: WB, IHC-Pmore details
    不适用于: Flow Cyt,ICC or IP
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Recombinant protein fragment corresponding to residues in Human Cystatin C.

  • 阳性对照
    • WB: Human testis, HeLa, HepG2, and U87-MG lysates. IHC-P: Human brain tissue.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.



Our Abpromise guarantee covers the use of ab109508 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/10000 - 1/50000. Detects a band of approximately 13 kDa (predicted molecular weight: 15 kDa).
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • 应用说明Is unsuitable for Flow Cyt,ICC or IP.
  • 靶标

    • 功能As an inhibitor of cysteine proteinases, this protein is thought to serve an important physiological role as a local regulator of this enzyme activity.
    • 组织特异性Expressed in submandibular and sublingual saliva but not in parotid saliva (at protein level). Expressed in various body fluids, such as the cerebrospinal fluid and plasma. Expressed in highest levels in the epididymis, vas deferens, brain, thymus, and ovary and the lowest in the submandibular gland.
    • 疾病相关Amyloidosis 6
      Macular degeneration, age-related, 11
    • 序列相似性Belongs to the cystatin family.
    • 翻译后修饰The Thr-25 variant is O-glycosylated with a core 1 or possibly core 8 glycan. The signal peptide of the O-glycosylated Thr-25 variant is cleaved between Ala-20 and Val-21.
    • 细胞定位Secreted.
    • Information by UniProt
    • 数据库链接
    • 别名
      • AD 8 antibody
      • AD8 antibody
      • Amyloid angiopathy and cerebral hemorrhage antibody
      • ARMD11 antibody
      • bA218C14.4 (cystatin C) antibody
      • bA218C14.4 antibody
      • Cst 3 antibody
      • Cst3 antibody
      • CST3 protein antibody
      • Cystatin 3 antibody
      • Cystatin-3 antibody
      • Cystatin-C antibody
      • Cystatin3 antibody
      • CystatinC antibody
      • CYTC_HUMAN antibody
      • Epididymis secretory protein Li 2 antibody
      • Gamma trace antibody
      • Gamma-trace antibody
      • HCCAA antibody
      • HEL S 2 antibody
      • MGC117328 antibody
      • Neuroendocrine basic polypeptide antibody
      • Post gamma globulin antibody
      • Post-gamma-globulin antibody
      see all

    Anti-Cystatin C antibody [EPR4413] 图像

    • All lanes : Anti-Cystatin C antibody [EPR4413] (ab109508) at 1/10000 dilution

      Lane 1 : Human testis lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : HepG2 cell lysate
      Lane 4 : U87-MG cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size : 15 kDa
      Observed band size : 13 kDa (why is the actual band size different from the predicted?)
    • Immunohistochemical analysis of paraffin-embedded Human brain tissue using ab109508 at adilution of 1/250.

    Anti-Cystatin C antibody [EPR4413] (ab109508)参考文献

    This product has been referenced in:
    • Wang T  et al. Lycium barbarum polysaccharide prevents focal cerebral ischemic injury by inhibiting neuronal apoptosis in mice. PLoS One 9:e90780 (2014). WB, IHC-P ; Mouse . Read more (PubMed: 24595452) »
    • Spanu S  et al. Urinary exosomes: a novel means to non-invasively assess changes in renal gene and protein expression. PLoS One 9:e109631 (2014). IHC ; Rat . Read more (PubMed: 25310591) »

    See all 2 Publications for this product

    Product Wall

    Application Immunohistochemistry (Frozen sections)
    Sample Mouse Tissue sections (Brain)
    Permeabilization Yes - 0.1 M PBS with 3% Triton X
    Specification Brain
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
    Fixative Paraformaldehyde

    Dr. Ruma Raha-Chowdhury

    Verified customer

    提交于 Aug 20 2015