Anti-CYP7A1抗体- C-terminal (ab79847)

概述

  • 产品名称
    Anti-CYP7A1抗体- C-terminal
    参阅全部 CYP7A1 一抗
  • 描述
    兔多克隆抗体to CYP7A1 - C-terminal
  • 经测试应用
    适用于: ELISA, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide (Human) conjugated to KLH from the C-terminus

  • 阳性对照
    • K562 cell lysate

应用

Our Abpromise guarantee covers the use of ab79847 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/1000.
WB 1/50 - 1/100. Detects a band of approximately 58 kDa (predicted molecular weight: 58 kDa).

靶标

  • 功能
    Catalyzes a rate-limiting step in cholesterol catabolism and bile acid biosynthesis by introducing a hydrophilic moiety at position 7 of cholesterol. Important for cholesterol homeostasis.
  • 组织特异性
    Detected in liver.
  • 通路
    Lipid metabolism; bile acid biosynthesis.
  • 序列相似性
    Belongs to the cytochrome P450 family.
  • 细胞定位
    Endoplasmic reticulum membrane. Microsome membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Cholesterol 7 alpha hydroxylase antibody
    • Cholesterol 7 alpha monooxygenase antibody
    • Cholesterol 7-alpha-hydroxylase antibody
    • Cholesterol 7-alpha-monooxygenase antibody
    • CP7A antibody
    • CP7A1_HUMAN antibody
    • CYP 7 antibody
    • CYP7 antibody
    • CYP7A1 antibody
    • CYPVII antibody
    • Cytochrome P450 7A1 antibody
    • Cytochrome P450, family 7, subfamily A, polypeptide 1 antibody
    • Cytochrome P450, subfamily VIIA (cholesterol 7 alpha monooxygenase), antibody
    • Cytochrome P450, subfamily VIIA (cholesterol 7 alpha-monooxygenase), polypeptide 1 antibody
    • MGC126826 antibody
    • MGC138389 antibody
    see all

图片

  • Anti-CYP7A1 antibody - C-terminal (ab79847) at 1/50 dilution + K562 cell lysates at 35 µg

    Predicted band size : 58 kDa
    Observed band size : 58 kDa
    Additional bands at : 30 kDa,38 kDa. We are unsure as to the identity of these extra bands.

实验方案

文献

This product has been referenced in:
  • Cai D  et al. Maternal Betaine Supplementation throughout Gestation and Lactation Modifies Hepatic Cholesterol Metabolic Genes in Weaning Piglets via AMPK/LXR-Mediated Pathway and Histone Modification. Nutrients 8: (2016). Read more (PubMed: 27763549) »
  • Hu Y  et al. In Ovo injection of betaine affects hepatic cholesterol metabolism through epigenetic gene regulation in newly hatched chicks. PLoS One 10:e0122643 (2015). WB ; Chicken . Read more (PubMed: 25860502) »

See all 6 Publications for this product

客户评价及客户问答

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Liver - Hepatocellular carcinoma and normal adjace)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citric acid 10mM pH6
Permeabilization
No
Specification
Liver - Hepatocellular carcinoma and normal adjace
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 23°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

提交于 Sep 29 2017

I have asked the lab to arrange for the blocking peptide to be made available. They have informed me that this should be on the catalog tomorrow with the number ab172639 and name Human CYP7A1 peptide.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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