快线抗体 仍未被充份测试和鉴定, 不在质保范围内, 具体请参见 使用条款及细节

概述

应用

Fast track antibodies constitute a diverse group of products that have been released to accelerate your research, but are not yet fully characterized. They have all been affinity purified and show high titre values against the immunizing peptide (by ELISA). Fast track terms of use

应用 Ab评论 说明
WB 1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 58 kDa).

靶标

  • 功能
    Catalyzes a rate-limiting step in cholesterol catabolism and bile acid biosynthesis by introducing a hydrophilic moiety at position 7 of cholesterol. Important for cholesterol homeostasis.
  • 组织特异性
    Detected in liver.
  • 通路
    Lipid metabolism; bile acid biosynthesis.
  • 序列相似性
    Belongs to the cytochrome P450 family.
  • 细胞定位
    Endoplasmic reticulum membrane. Microsome membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Cholesterol 7 alpha hydroxylase antibody
    • Cholesterol 7 alpha monooxygenase antibody
    • Cholesterol 7-alpha-hydroxylase antibody
    • Cholesterol 7-alpha-monooxygenase antibody
    • CP7A antibody
    • CP7A1_HUMAN antibody
    • CYP 7 antibody
    • CYP7 antibody
    • CYP7A1 antibody
    • CYPVII antibody
    • Cytochrome P450 7A1 antibody
    • Cytochrome P450, family 7, subfamily A, polypeptide 1 antibody
    • Cytochrome P450, subfamily VIIA (cholesterol 7 alpha monooxygenase), antibody
    • Cytochrome P450, subfamily VIIA (cholesterol 7 alpha-monooxygenase), polypeptide 1 antibody
    • MGC126826 antibody
    • MGC138389 antibody
    see all

图片

This Fast-Track antibody is not yet fully characterised. These images represent inconclusive preliminary data.

  • All lanes : Anti-CYP7A1 antibody (ab65596) at 1/1000 dilution

    All lanes : Mouse liver lysate

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP labeled goat anti-rabbit at 1/5000 dilution

    Developed using the ECL technique.

    Performed under non-reducing conditions.

    Predicted band size: 58 kDa
    Additional bands at: 40 kDa (possible non-specific binding), 70 kDa (possible non-specific binding)



    Blocking: 5% Milk

    See Abreview

实验方案

文献

This product has been referenced in:
  • Yetti H  et al. Bile acid detoxifying enzymes limit susceptibility to liver fibrosis in female SHRSP5/Dmcr rats fed with a high-fat-cholesterol diet. PLoS One 13:e0192863 (2018). WB ; Rat . Read more (PubMed: 29438418) »
  • Yuan Y  et al. Combination of Hypertension Along with a High Fat and Cholesterol Diet Induces Severe Hepatic Inflammation in Rats via a Signaling Network Comprising NF-?B, MAPK, and Nrf2 Pathways. Nutrients 9:N/A (2017). WB ; Rat . Read more (PubMed: 28906458) »

See all 11 Publications for this product

客户评价及客户问答

Application
Western blot
Sample
Mouse Tissue lysate - whole (liver)
Gel Running Conditions
Non-reduced Denaturing (12%)
Loading amount
30 µg
Specification
liver
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Username

Abcam user community

Verified customer

提交于 Jul 05 2017

Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Reduced Denaturing (7.5)
Sample
Rat Tissue lysate - whole (liver)
Specification
liver
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 7.5%
Username

Abcam user community

Verified customer

提交于 Sep 09 2013



ab65596 es un llamado “Fast Track” antibody. Se trata de anticuerpos que no se han caracterizado completamente, y que se han añadido al catálogo antes de realizar la validación completa por la necesidad urgent...

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Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hearthe customer hashad difficulty obtaining satisfactory results from this antibody.

The details you have kindly provided will enable us to investigat...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (liver)
Loading amount
30 µg
Specification
liver
Gel Running Conditions
Reduced Denaturing (4-20% gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Jan 19 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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