概述

  • 产品名称Anti-CYP4X1抗体
    参阅全部 CYP4X1 一抗
  • 描述
    兔多克隆抗体to CYP4X1
  • 特异性ab74810 detects endogenous levels of total CYP4X1 protein.
  • 经测试应用适用于: ELISA, WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    A synthetic peptide derived from an internal sequence of human CYP4X1.

  • 阳性对照
    • IHC-P: human liver carcinoma tissue. WB: Extracts from COS7 cells.

性能

应用

Our Abpromise guarantee covers the use of ab74810 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/40000.
WB 1/500 - 1/1000. Predicted molecular weight: 59 kDa.
IHC-P 1/50 - 1/100.

靶标

  • 相关性CYP4X1 is a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. The expression pattern of a similar rat protein suggests that this protein may be involved in neurovascular function in the brain. Catalytic activity: RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.
  • 细胞定位Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.
  • 数据库链接
  • 别名
    • CYPIVX1 antibody
    • Cytochrome P450 4X1 antibody
    • Cytochrome P450, family 4, subfamily X, polypeptide 1 antibody

Anti-CYP4X1 antibody 图像

  • ab74810, at 1/50 dilution, staining CYP4X1 in human liver carcinoma tissue sections by Immunohistochemistry in the absence (left image) and presence (right image) of the immunising peptide.
  • All lanes : Anti-CYP4X1 antibody (ab74810) at 1/500 dilution

    Lane 1 : extracts from COS78 cells
    Lane 2 : extracts from COS78 cells with immunising peptide at 5 µg

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 59 kDa
    Observed band size : 53 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 29 kDa. We are unsure as to the identity of these extra bands.

Anti-CYP4X1 antibody (ab74810)参考文献

ab74810 has not yet been referenced specifically in any publications.

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