Detects endogenous levels of total CYP4B1 protein
A synthesized peptide derived from an internal sequence of human CYP4B1.
Extracts from COLO cells; human lung carcinoma tissue
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Preservative: 0.02% Sodium azide Constituents: PBS, 0.88% Sodium chloride, 50% Glycerol
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Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Predicted molecular weight: 58 kDa.
1/50 - 1/100.
Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.
Detected in the liver and lung (at protein level).
Belongs to the cytochrome P450 family.
Endoplasmic reticulum membrane. Microsome membrane.
Information by UniProt
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - CYP4B1 antibody (ab110012)
Immunohistochemistry analysis of paraffin embedded human lung carcinoma tissue, using ab110012 at 1/50. The picture on the right is treated with the synthesized peptide.
Western blot - CYP4B1 antibody (ab110012)
All lanes : Anti-CYP4B1 antibody (ab110012) at 1/500 dilution Lane 1 : extracts from COLO cells (5-30 ug total protein) with no immunizing peptide Lane 2 : extracts from COLO cells (5-30 ug total protein) with immunizing peptide (5-10 ug) Predicted band size: 58 kDa
has not yet been referenced specifically in any publications.
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