Anti-CYP2U1抗体(ab65128)
Key features and details
- Rabbit polyclonal to CYP2U1
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-CYP2U1抗体
参阅全部 CYP2U1 一抗 -
描述
兔多克隆抗体to CYP2U1 -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IFmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat -
免疫原
Synthetic peptide (Human) from an internal sequence
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阳性对照
- HeLa and LOVO cell extracts
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab65128于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/500 - 1/1000. Detects a band of approximately 62 kDa (predicted molecular weight: 62 kDa).
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ICC/IF |
Use a concentration of 1 - 5 µg/ml.
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说明 |
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WB
1/500 - 1/1000. Detects a band of approximately 62 kDa (predicted molecular weight: 62 kDa). |
ICC/IF
Use a concentration of 1 - 5 µg/ml. |
靶标
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功能
Catalyzes the hydroxylation of arachidonic acid, docosahexaenoic acid and other long chain fatty acids. May modulate the arachidonic acid signaling pathway and play a role in other fatty acid signaling processes. -
组织特异性
Widely expressed with stronger expression in thymus, heart and cerebellum. -
序列相似性
Belongs to the cytochrome P450 family. -
发展阶段
Expressed in fetal thymus. -
细胞定位
Endoplasmic reticulum membrane. Microsome membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 113612 Human
- Entrez Gene: 71519 Mouse
- Entrez Gene: 310848 Rat
- Omim: 610670 Human
- SwissProt: Q7Z449 Human
- SwissProt: Q9CX98 Mouse
- SwissProt: Q4V8D1 Rat
- Unigene: 109087 Human
see all -
别名
- CP2U1_HUMAN antibody
- CYP2U 1 antibody
- CYP2U1 antibody
see all
图片
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All lanes : Anti-CYP2U1 antibody (ab65128) at 1/500 dilution
Lane 1 : HeLa cell extract
Lane 2 : LOVO cell extract
Lane 3 : LOVO cell extract with immunising peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size: 62 kDa
Observed band size: 62 kDa
Additional bands at: 28 kDa, 55 kDa. We are unsure as to the identity of these extra bands. -
ICC/IF image of ab65128 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab65128, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab65128 被引用在 3 文献中.
- Legrand A et al. Pseudoxanthoma elasticum overlaps hereditary spastic paraplegia type 56. J Intern Med 289:709-725 (2021). PubMed: 33107650
- Yu X et al. Glutamate affects the CYP1B1- and CYP2U1-mediated hydroxylation of arachidonic acid metabolism via astrocytic mGlu5 receptor. Int J Biochem Cell Biol 110:111-121 (2019). PubMed: 30858141
- Durand CM et al. CYP2U1 activity is altered by missense mutations in hereditary spastic paraplegia 56. Hum Mutat 39:140-151 (2018). PubMed: 29034544