ab69650 detects endogenous levels of total CYP2S1 protein.
Synthetic peptide derived from an internal sequence of human CYP2S1
Extracts from HUVEC and COLO cells, A549 cells and human lung carcinoma tissue.
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg 2+ and Ca 2+), 150mM Sodium chloride, pH 7.4
Concentration information loading...
Immunogen affinity purified
ab69650 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa).
1/50 - 1/100.
1/500 - 1/1000.
Has a potential importance for extrahepatic xenobiotic metabolism.
High level of expression in trachea, lung, stomach, small intestine, and spleen.
Belongs to the cytochrome P450 family.
Endoplasmic reticulum membrane. Microsome membrane.
Information by UniProt
Immunocytochemistry/ Immunofluorescence - Anti-CYP2S1 antibody (ab69650)
ab69650 at 1/500 dilution staining CYP2S1 in A549 cells by Immunofluorescence, in the absence or presence of the immunising peptide
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP2S1 antibody (ab69650)
ab69650 at 1/50 dilution staining CYP2S1 in human lung carcinoma by Immunohistochemistry, Paraffin-embedded tissue, in the absence or presence of the immunising peptide.
Western blot - Anti-CYP2S1 antibody (ab69650)
All lanes : Anti-CYP2S1 antibody (ab69650) at 1/500 dilution Lane 1 : HUVEC cell extract Lane 2 : COLO cell extract Lane 3 : COLO cell extract with immunising peptide at 10 µg Lysates/proteins at 30 µg per lane. Predicted band size: 56 kDa Observed band size: 56 kDa
This product has been referenced in:
et al. Restitution of tumor suppressor microRNAs using a systemic nanovector inhibits pancreatic cancer growth in mice. Mol Cancer Ther 10:1470-80 (2011).
Read more (PubMed: 21622730) »
et al. CYP2S1 and CYP2W1 mediate 2-(3,4-dimethoxyphenyl)-5-fluorobenzothiazole (GW-610, NSC 721648) sensitivity in breast and colorectal cancer cells. Mol Cancer Ther 10:1982-92 (2011).
Read more (PubMed: 21831963) »
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"